Study on Toxicity of 9 Biocontrol Microbial Products to Adult Population of Tobacco Beetle,Lasioderrma serricorne(Fabricius)

[Objectives]The paper was to study the toxicity of 9 biocontrol microbial products to adult population of Lasioderrma serricorne.[Methods]Nine biocontrol microbes were used as test materials to study their toxicity to the experimental population of L.serricorne adults.[Results]The toxicities of these biocontrol microbes from low to high at 5 d post spraying were:Luhai Beauveria bassiana powder(LC_(50)=462.752×10^(8) spores/L),Meichongzhi B.bassiana powder(LC_(50)=9713.157×10^(8) spores/L),Yiqiang Bio B.bassiana powder(LC_(50)=11203.321×10^(8)spores/L),Nongbao Bio B.bassiana powder(LC_(50)=12188.866×10^(8) spores/L),Yeshengwang B.bassiana powder(LC_(50)=21685.532×10^(8) spores/L);Fatu Bacillus thuringiensis suspension(LC_(50)=1.0844×10^(8) IU/L),Laojite B.thuringiensis suspension(LC_(50)=2.056×10^(8) IU/L),Lujinwa B.thuringiensis powder(LC_(50)=2.273×10^(8) IU/L),Nongbao Bio B.thuringiensis powder(LC_(50)=18.399×10^(8) spores/L).The toxicities of these biocontrol microbes from low to high at 10 d post spraying were:Nongbao Bio B.bassiana powder(LC_(50)=0.072×10^(8) spores/L),Yiqiang Bio B.bassiana powder(LC_(50)=2.484×10^(8) spores/L),Luhai B.bassiana powder(LC_(50)=44.551×10^(8) spores/L),Meichongzhi B.bassiana powder(LC_(50)=96.447×10^(8) spores/L),Yeshengwang B.bassiana powder(LC_(50)=723.347×10^(8) spores/L);Lujinwa B.thuringiensis powder(LC_(50)=0.0001×10^(8) IU/L),Fatu B.thuringiensis suspension(LC_(50)=0.045×10^(8) IU/L),Laojite B.thuringiensis suspension(LC_(50)=0.0644×10^(8) IU/L),Nongbao Bio B.thuringiensis powder(LC_(50)=1.899×10^(8) spores/L).The toxicities of these biocontrol microbes from low to high at 15 d post spraying were:Meichongzhi B.bassiana powder(LC_(50)=0.001×10^(8) spores/L),Nongbao Bio B.bassiana powder(LC_(50)=0.01×10^(8) spores/L),Yiqiang Bio B.bassiana powder(LC_(50)=0.084×10^(8) spores/L),Luhai B.bassiana powder(LC_(50)=2.370×10^(8) spores/L),Yeshengwang B.bassiana powder(LC_(50)=8.915×10^(8)spores/L);Lujinwa B.thuringiensis powder(LC_(50)=0.16×10^(4) IU/L),Laojite B.thuringiensis suspension(LC_(50)=0.185×10^(4) IU/L),Fatu B.thuringiensis suspension(LC_(50)=32.211×10^(4) IU/L),Nongbao Bio B.thuringiensis powder(LC_(50)=1590×10^(4) spores/L).[Conclusions]According to the three stages of tube rubbing,it is found that the control effects of Lujinwa B.thuringiensis powder and Meichongzhi B.bassiana powder are the best,and the results will provide technical support for the biological control of L.serricorne adults.

Occurrence Regularity and Life History of Cigarette Beetle Lasioderrma serricorne(Fabricius)in Tobacco Leaf Threshing and Redrying Workshop

[Objectives]The paper was to study the occurrence regularity and life history of cigarette beetle Lasioderrma serricorne(Fabricius)in tobacco leaf threshing and redrying workshop.[Methods]With the tobacco leaf threshing and redrying workshop as the survey site,the occurrence regularity of L.serricorne was investigated,and the life history table was inferred according to its biological characteristics.The temperature,humidity and duration of tobacco leaf threshing and redrying were simulated in the laboratory to study the survival rate of all states of L.serricorne in this environment.[Results]The lifecycle of L.serricorne in tobacco leaf threshing and redrying workshop was:the overwintering generation pupated in late February;the pupa began to emerge in early March,and reached its peak in late March.The first-generation eggs started hatching in late March,pupated in late May,emerged in early June,and reached the peak eclosion of the first-generation adults in mid-July.The second-generation eggs began to hatch in late July,pupated in mid-August,emerged in late August,and reached the peak eclosion of the second-generation adults in mid-September.The third-generation eggs began to hatch in mid-September;most of the larvae began to overwinter,some pupated in mid-November and survived the winter as pupae,and some pupae emerged to adults.The survival test results of different states of L.serricorne under simulated temperature,humidity and duration in the tobacco leaf threshing and redrying process showed that the mortality rates of eggs in simulated 1,2 and 3 conditions were about 51.22%,90.24% and 100%,and the mortalities of larvae in simulated 1,2 and 3 conditions were about 18.30%,81.25%and 100%,respectively.The mortalities of pupae in simulated 1,2 and 3 conditions were about 69.39%,100% and 100%,and the mortalities of adults in simulated 1,2 and 3 conditions were about 100%,100% and 100%,respectively.[Conclusions]L.serricorne of different states can be killed by appropriately raising the temperature during threshing and redrying.