Elateridae（click beetles） is the largest family within the Elateroidea and is abundant worldwide. However, their phylogenetic relationships remain uncertain; older studies based on the external morphology of adults ...Elateridae（click beetles） is the largest family within the Elateroidea and is abundant worldwide. However, their phylogenetic relationships remain uncertain; older studies based on the external morphology of adults and larvae led to divergent classification systems. Many tribes, genera, and even subfamilies remain controversial. Partial sequences of 28S ribosomal DNA（rDNA） were used to investigate the phylogeny of click beetles. Sequences of 28S rDNA from 80 different click beetle species collected from different locations across China were determined along with those of another 86 Elateridae species and 14 outgroup species. The aligned data were analyzed by neighbor joining, maximum parsimony, maximum likelihood, and Bayesian analyses. All methods produced a nearly consistent basal topology that was partially congruent with those of previous studies based on adult and larval morphology as well as molecular data. Overall, the monophyly of Elateridae was supported. The family was divided into five clades, Denticollinae, Negastriinae, Cardiophorinae, Pyrophorinae, Oestodinae, and Elaterinae. Senodonia is traditionally considered a member of Elateridae, but this study indicated that it was more closely related to Lycidae. The position of the outgroup family Lampyridae also differed from its placement in previous research. In addition to confirming the Elateridae as monophyletic, we concluded that Oxynopterinae, Pityobiinae, and Hypnoidinae should be merged into Denticollinae; Conoderinae and Agrypninae should be regarded as subgroups of Pyrophorinae; and Melanotinae should be classified as a tribe of Elaterinae. Additional species should be sampled in future studies to confirm this reclassification.展开更多
基金supported by the National Natural Science Foundation of China (31772511)Guizhou Science and Technology Cooperation Project(QianKeHe LH Zi [2016]7214)+1 种基金Earmarked Fund for Construction of the Key Laboratory for Conservation and Innovation of Buckwheat Germplasm in Guizhou(QianJiaoHe KY Zi [2017] 002)the Doctoral Research Project of Guizhou Normal University
文摘Elateridae（click beetles） is the largest family within the Elateroidea and is abundant worldwide. However, their phylogenetic relationships remain uncertain; older studies based on the external morphology of adults and larvae led to divergent classification systems. Many tribes, genera, and even subfamilies remain controversial. Partial sequences of 28S ribosomal DNA（rDNA） were used to investigate the phylogeny of click beetles. Sequences of 28S rDNA from 80 different click beetle species collected from different locations across China were determined along with those of another 86 Elateridae species and 14 outgroup species. The aligned data were analyzed by neighbor joining, maximum parsimony, maximum likelihood, and Bayesian analyses. All methods produced a nearly consistent basal topology that was partially congruent with those of previous studies based on adult and larval morphology as well as molecular data. Overall, the monophyly of Elateridae was supported. The family was divided into five clades, Denticollinae, Negastriinae, Cardiophorinae, Pyrophorinae, Oestodinae, and Elaterinae. Senodonia is traditionally considered a member of Elateridae, but this study indicated that it was more closely related to Lycidae. The position of the outgroup family Lampyridae also differed from its placement in previous research. In addition to confirming the Elateridae as monophyletic, we concluded that Oxynopterinae, Pityobiinae, and Hypnoidinae should be merged into Denticollinae; Conoderinae and Agrypninae should be regarded as subgroups of Pyrophorinae; and Melanotinae should be classified as a tribe of Elaterinae. Additional species should be sampled in future studies to confirm this reclassification.
