The CmBBX8-CmFTL1 regulatory module is a key determinant in the transition from vegetative growth to reproductive development in summer-flowering chrysanthemum.However,the detailed regulatory mechanism of CmBBX8-media...The CmBBX8-CmFTL1 regulatory module is a key determinant in the transition from vegetative growth to reproductive development in summer-flowering chrysanthemum.However,the detailed regulatory mechanism of CmBBX8-mediated flowering remains elusive.In this study,we revealed that RADICAL-INDUCED CELL DEATH 1(CmRCD1)physically associated with CmBBX8 through bimolecular fluorescence complementation(BiFC),pulldown and Coimmunoprecipitation(CoIP)assays.Furthermore,the RCD1-SRO1-TAF4(RST)domain of CmRCD1 and the B-box of CmBBX8 mediated their interaction.In addition,Luciferase(LUC)assays and electrophoretic mobility shift assay(EMSAs)showed that CmRCD1 repressed the transcriptional activity of CmBBX8 and interfered with its binding to the CmFTL1 promoter,thereby leading to delayed flowering in the summer chrysanthemum‘Yuuka’.These results provide insight into the molecular framework of CmRCD1-CmBBX8-mediated flowering in chrysanthemum.展开更多
基金the National Natural Science Foundation of China(31930100,31872146)a project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions.
文摘The CmBBX8-CmFTL1 regulatory module is a key determinant in the transition from vegetative growth to reproductive development in summer-flowering chrysanthemum.However,the detailed regulatory mechanism of CmBBX8-mediated flowering remains elusive.In this study,we revealed that RADICAL-INDUCED CELL DEATH 1(CmRCD1)physically associated with CmBBX8 through bimolecular fluorescence complementation(BiFC),pulldown and Coimmunoprecipitation(CoIP)assays.Furthermore,the RCD1-SRO1-TAF4(RST)domain of CmRCD1 and the B-box of CmBBX8 mediated their interaction.In addition,Luciferase(LUC)assays and electrophoretic mobility shift assay(EMSAs)showed that CmRCD1 repressed the transcriptional activity of CmBBX8 and interfered with its binding to the CmFTL1 promoter,thereby leading to delayed flowering in the summer chrysanthemum‘Yuuka’.These results provide insight into the molecular framework of CmRCD1-CmBBX8-mediated flowering in chrysanthemum.
