Human cytomegalovirus virions contain three major glycoprotein complexes (gC I, II, III), all of which are required for CMV infectivity. These complexes also represent major antigenic targets for anti-viral immune res...Human cytomegalovirus virions contain three major glycoprotein complexes (gC I, II, III), all of which are required for CMV infectivity. These complexes also represent major antigenic targets for anti-viral immune responses. The gC II complex consists of two glycoproteins, gM and gN. In the current study, DNA vaccines expressing the murine cytomegalovirus (MCMV) homologs of the gM and gN proteins were evaluated for protection against lethal MCMV infection in a mouse model. Humoral and cellular immune responses, spleen viral titers, and mice survival and body-weight changes were examined. The results showed that immunization with gM or gN DNA vaccine alone was not able to offer good protection, whereas co-immunization with both gM and gN induced an effective neutralizing antibody response and cellular immune response, and provided mice with complete protection against a lethal MCMV challenge. This study provides the first in vivo evidence that the gC II (gM-gN) complex may be able to serve as a protective subunit antigen for future HCMV vaccine development.展开更多
There are few studies on the genetic evolution of Curcuma L.,and it is easy to have synonyms or homonyms.In order to make better development and utilization of Curcuma L.,by consulting the relevant literature,the mole...There are few studies on the genetic evolution of Curcuma L.,and it is easy to have synonyms or homonyms.In order to make better development and utilization of Curcuma L.,by consulting the relevant literature,the molecular biology and genetics of Curcuma L.were summarized,and discussed in this paper,in order to lay a foundation for the study of phylogeny and genetic evolution of Curcuma L.展开更多
To explore the impact of gene polymorphism of human cytomegalovirus(HCMV)on virus virulence,the full-length UL144 gene and partial sequence of glycoprotein B(UL55)gene were sequenced and analyzed for 23clinical strain...To explore the impact of gene polymorphism of human cytomegalovirus(HCMV)on virus virulence,the full-length UL144 gene and partial sequence of glycoprotein B(UL55)gene were sequenced and analyzed for 23clinical strains of HCMV isolated from urine samples of pediatric patients with congenital or postnatal HCMV infection.Among the 23 isolates,13(57%)were UL144genotype 1A,3(13%)were UL144 genotypes 2 and 7(30%)were UL144 genotype 3;geographic differences in genotype distribution were found for both UL144 and gB gene.No UL144 genotypes 1B and 1C were found in this study while these two genotypes were common in HCMV strains isolated in the US.Our results also demonstrated that for all clinical strains of gB genotypes I,III and UL144genotypes 1A,2,and 3 found in this study,mother-to-fetus vertical transmission was possible.展开更多
Jishou Municipal Center for Disease Control and Prevention(Jishou CDC)received a phone call at 7:30 a.m.on April 24,2020 from Xiangxi Tujia and Miao Autonomous Prefectural CDC(Xiangxi CDC)that avian influenza A/H9N2 v...Jishou Municipal Center for Disease Control and Prevention(Jishou CDC)received a phone call at 7:30 a.m.on April 24,2020 from Xiangxi Tujia and Miao Autonomous Prefectural CDC(Xiangxi CDC)that avian influenza A/H9N2 virus was detected by quantitative reverse transcription-polymerase chain reaction(RT-PCR)from the throat swab sample of an influenza-like illness in an outpatient in the Xiangxi Traditional Chinese Medicine Hospital.展开更多
Dear Editor,Since August 2010,the world was in the post-pandemic period and the influenza A(H1N1)pdm09(pdmH1N1)virus co-circulated with H3N2 and B virus seasonally(WHO,2010).The continuous surveillance on the pdmH1N1 ...Dear Editor,Since August 2010,the world was in the post-pandemic period and the influenza A(H1N1)pdm09(pdmH1N1)virus co-circulated with H3N2 and B virus seasonally(WHO,2010).The continuous surveillance on the pdmH1N1 virus in the post-pandemic period was recommended by WHO.In order to detect unusual events that might indicate a shift in severity disease,or emergence of a new virus,we collected a total of 312 throat swabs of laboratory-confirmed pdmHlNl patients from Hunan and Jilin provinces in展开更多
基金supported by the Innovation Platform Open Fund of Hunan Provincial Education Department (11K010)a research fund from Hunan Provincial Science and Technology Development (2008TP4033-2)
文摘Human cytomegalovirus virions contain three major glycoprotein complexes (gC I, II, III), all of which are required for CMV infectivity. These complexes also represent major antigenic targets for anti-viral immune responses. The gC II complex consists of two glycoproteins, gM and gN. In the current study, DNA vaccines expressing the murine cytomegalovirus (MCMV) homologs of the gM and gN proteins were evaluated for protection against lethal MCMV infection in a mouse model. Humoral and cellular immune responses, spleen viral titers, and mice survival and body-weight changes were examined. The results showed that immunization with gM or gN DNA vaccine alone was not able to offer good protection, whereas co-immunization with both gM and gN induced an effective neutralizing antibody response and cellular immune response, and provided mice with complete protection against a lethal MCMV challenge. This study provides the first in vivo evidence that the gC II (gM-gN) complex may be able to serve as a protective subunit antigen for future HCMV vaccine development.
基金Zhangzhou Nature Foundation:Study on the Genetic Relationship of Curcuma alismatifolia Germplasm Resources Based on Pollen Morphology and RAD-seq(ZZ2020J17).
文摘There are few studies on the genetic evolution of Curcuma L.,and it is easy to have synonyms or homonyms.In order to make better development and utilization of Curcuma L.,by consulting the relevant literature,the molecular biology and genetics of Curcuma L.were summarized,and discussed in this paper,in order to lay a foundation for the study of phylogeny and genetic evolution of Curcuma L.
基金supported by the Innovation Platform Open Funds for Universities in Hunan Province(09K022and 11K010)the Cooperative Innovation Center of Engineering and New Products for Developmental Biology of Hunan Province
文摘To explore the impact of gene polymorphism of human cytomegalovirus(HCMV)on virus virulence,the full-length UL144 gene and partial sequence of glycoprotein B(UL55)gene were sequenced and analyzed for 23clinical strains of HCMV isolated from urine samples of pediatric patients with congenital or postnatal HCMV infection.Among the 23 isolates,13(57%)were UL144genotype 1A,3(13%)were UL144 genotypes 2 and 7(30%)were UL144 genotype 3;geographic differences in genotype distribution were found for both UL144 and gB gene.No UL144 genotypes 1B and 1C were found in this study while these two genotypes were common in HCMV strains isolated in the US.Our results also demonstrated that for all clinical strains of gB genotypes I,III and UL144genotypes 1A,2,and 3 found in this study,mother-to-fetus vertical transmission was possible.
基金We thank the colleagues from the Chinese National Influenza Center,who isolated and sequenced the full genome of A/Hunan/11173/2020(H9N2).
文摘Jishou Municipal Center for Disease Control and Prevention(Jishou CDC)received a phone call at 7:30 a.m.on April 24,2020 from Xiangxi Tujia and Miao Autonomous Prefectural CDC(Xiangxi CDC)that avian influenza A/H9N2 virus was detected by quantitative reverse transcription-polymerase chain reaction(RT-PCR)from the throat swab sample of an influenza-like illness in an outpatient in the Xiangxi Traditional Chinese Medicine Hospital.
基金supported by the National Mega-projects for Infectious Diseases(2014ZX10004002-002 to Yuelong Shu)the National Key Research and Development Program of China(2016YFC1200201 to Yuelong Shu)the National Nature Science Foundation of China(81601758 to Wenfei Zhu)
文摘Dear Editor,Since August 2010,the world was in the post-pandemic period and the influenza A(H1N1)pdm09(pdmH1N1)virus co-circulated with H3N2 and B virus seasonally(WHO,2010).The continuous surveillance on the pdmH1N1 virus in the post-pandemic period was recommended by WHO.In order to detect unusual events that might indicate a shift in severity disease,or emergence of a new virus,we collected a total of 312 throat swabs of laboratory-confirmed pdmHlNl patients from Hunan and Jilin provinces in
