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Converting Escherichia coli MG1655 into a chemical overproducer through inactivating defense system against exogenous DNA 被引量:2
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作者 Jingge Wang chaoyong huang +4 位作者 Kai Guo Lianjie Ma Xiangyu Meng Ning Wang Yi-Xin Huo 《Synthetic and Systems Biotechnology》 SCIE 2020年第4期333-342,共10页
Escherichia coli strain K-12 MG1655 has been proposed as an appropriate host strain for industrial production.However,the direct application of this strain suffers from the transformation inefficiency and plasmid inst... Escherichia coli strain K-12 MG1655 has been proposed as an appropriate host strain for industrial production.However,the direct application of this strain suffers from the transformation inefficiency and plasmid instability.Herein,we conducted genetic modifications at a serial of loci of MG1655 genome,generating a robust and universal host strain JW128 with higher transformation efficiency and plasmid stability that can be used to efficiently produce desired chemicals after introducing the corresponding synthetic pathways.Using JW128 as the host,the titer of isobutanol reached 5.76 g/L in shake-flask fermentation,and the titer of lycopene reached 1.91 g/L in test-tube fermentation,40-fold and 5-fold higher than that of original MG1655,respectively.These results demonstrated JW128 is a promising chassis for high-level production of value-added chemicals. 展开更多
关键词 Escherichia coli MG1655 CRISPR-Cas9 Defense system Metabolic engineering
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