In vitro anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe grade black tea(Camellia sinensis L.)

Objective:To access the anti-hyaluronidase activity of Sri Lankan low grown orthodox orange pekoe(OP) grade black tea with a view to develop an anti-aging skin formulation.Methods:Five concentrations(0.125,0.250,0.500,1.000 and 2.000 mg/mL) of black tea brew(BTB) were made using a freeze dried sample of Sri Lankan low grown orthodox OP grade black tea which was prepared according to international organization for standardization specification.Epigallocatechin gallate(EGCG) was used as the reference agent(concentrations tested:0.012,0.025,0.050,0.100 and 0.200 mg/mL).Anti-hyaluronidase activity of BTB and EGCG in vitro were ascertained spectrometrically using hyaluronic acid(from rooster comb)and bovine testicular hyaluronidase.Results:The results revealed that BTB had moderate[IC_(50)=(1.09±0.12) mg/mL]and dose dependent(r^2=0.94) anti-hyaluronidase activity.EGCG also exhibited dose dependent(r^2=0.93,P<0.05) anti-hyaluronidase activity which was superior[IC_(50)=(0.09±0.00) mg/mL]to BTB.Conclusions:Sri Lankan low grown orthodox OP gradc black tca has promising antihyaluronidase activity in vitro and has the potential to be used as an anti-aging cosmaceutical.In addition,it may prove useful as a beverage in the management of allergy,some joint diseases and cnvenomation.

In vitro pancreatic lipase,cholesterol esterase and cholesterol micellization inhibitory activity of Sri Lankan low grown orthodox Orange Pekoe grade black tea(Camellia sinensis L.)

Objective:To access the pancreatic lipase,cholesterol esterase and cholesterol micellization inhibitory activities of Sri Lankan low grown orthodox Orange Pekoe grade black tea made from uppermost tender leaves and unopened buds of Camellia sinensis L.Methods:Black tea brew(BTB)was made according to International Organization for Standardization 3103 specifications and concentrations of BTB tested were 37.5,75.0,150.0,300.0 and 600.0μg/mL for antilipase and anti-cholesterol esterase assays and 0.25,0.50 and 1.00μg/mL for cholesterol micellization inhibitory assay.Results:The results showed that BTB of Sri Lankan low grown orthodox Orange Pekoe grade black tea has marked and dose-dependent(r^(2)=0.95)cholesterol micellization inhibitory activity in vitro comparable to epigallocatechin gallate,the reference drug used.In contrast,BTB had only mild but dose-dependent(r^(2)=0.94)inhibitory activity against pancreatic lipase and weak inhibitory effect(up to 13.17%)on pancreatic cholesterol esterase.Conclusions:It is concluded that consumption of BTB of Sri Lankan low grown orthodox Orange Pekoe grade tea as a beverage may be a useful strategy in the management of hyperlipidaemia.

Sri Lankan black tea(Camellia sinensis L.)inhibits the methylglyoxal mediated protein glycation and potentiates its reversing activity in vitro

Objective:To evaluate inhibitory activity of methylglyoxal(MGO)mediated protein glycation and ability to potentiate its reversing activity and range of antioxidant properties of Sri Lankan low grown orthodox orange pekoe grade black tea.Methods:Freeze dried black tea brew(BTB)was used as the sample in this study.Antiglycation and glycation reversing activity was studied in bovine serum albumin(BSA)-MGO model.Antioxidant properties were studied using total polyphenolic content,total flavonoid content,2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid,1,1-diphenyl-2-picrylhydrazine and ferric reducing antioxidant power in vitro antioxidant assays.Results:The results demonstrated significant(P<0.05)and dose dependant inhibition of BSA-MGO glycation[IC_(50):(164.30±4.85)μg/mL],potentiating of its reversing activity[EC_(50):(235.39±5.37)μg/mL]and marked antioxidant properties[total polyphenolic content:(119.55±9.97)mg gallic acid equivalents/g BTB;total flavonoid content:(6.04±1.26)mg quercetin equivalents/g BTB;2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid,1,1-diphenyl-2-picrylhydrazine and ferric reducing antioxidant power:(3.29±0.06),(1.95±0.15)and(1.31±0.19)mmol Trolox equivalents/g BTB,respectively].No correlations were observed between antioxidant activity and BSA-MGO glycation.Conclusions:The novel properties observed for Sri Lankan orange pekoe grade black tea indicate its usefulness as a supplementary beverage in managing MGO and advanced glycation end products related diseases and ailments.