目的:通过对单中心大样本慢性乙型肝炎病毒(hepatitis B virus,HBV)感染队列的分析,对我国慢性HBV感染自然病程的划分提出修订建议。方法:回顾性地纳入2014年1月至2020年10月在中国人民解放军总医院第五医学中心接受过肝组织活检的慢性...目的:通过对单中心大样本慢性乙型肝炎病毒(hepatitis B virus,HBV)感染队列的分析,对我国慢性HBV感染自然病程的划分提出修订建议。方法:回顾性地纳入2014年1月至2020年10月在中国人民解放军总医院第五医学中心接受过肝组织活检的慢性HBV感染者。参考《欧洲肝病学会乙型肝炎病毒感染管理临床实践指南(2017年版)》等国内外最新版慢性乙型肝炎(chronic hepatitis B,CHB)防治指南,将患者按乙型肝炎e抗原(hepatitis B e antigen,HBeAg)状态及肝损伤程度分为HBeAg阳性感染(免疫耐受期)、HBeAg阳性CHB(免疫清除期)、HBeAg阴性感染(免疫控制期)和HBeAg阴性CHB(再活动期)四个自然病程分期,并重点比较了不同分期患者的人口学和实验室检验结果。两组间年龄差异采用Mann-Whitney U检验。结果:最终纳入符合纳排标准的患者760例,包括197例未成年(年龄<18岁)和563例成年感染者,男性456例、女性304例,纳入患者的中位年龄为29岁,(四分位间距:16,39岁)。上述四个自然病程分期患者分别有173、329、95和163例,进一步比较四期患者的年龄发现:HBeAg阴性CHB的中位年龄尽管大于HBeAg阳性CHB(37岁vs.24岁,P<0.001),但却小于HBeAg阴性感染者(37岁vs.39岁,P=0.240)。结论:根据本研究可以推测,HBeAg阴性CHB患者并非都是由HBeAg阴性感染者进入再活动期发展而来,也可以由仍处于持续免疫活动状态的HBeAg阳性CHB患者发生HBeAg阴转或血清学转换而来。展开更多
Objective:To explore the potential of metanephric mesenchymal cells(MMCs)for osteogenesis and naringin’s ability to enhance this process and its molecular mechanism.Methods:Porcine MMCs at 70 days of gestation were u...Objective:To explore the potential of metanephric mesenchymal cells(MMCs)for osteogenesis and naringin’s ability to enhance this process and its molecular mechanism.Methods:Porcine MMCs at 70 days of gestation were used as tool cells,cultured in osteogenic induction medium,identified by immunocytochemistry staining.Osteogenic potential of porcine MMCs and naringin’s ability to enhance this process was tested by detecting changes in cell viability,alkaline phosphatase(ALP)activity,the expression of runt-related transcription factor 2(Runx2),osteopontin(OPN)and osteocalcin(OCN),and the formation of mineralized nodules,and the application of the p38 signaling pathway inhibitor SB203580 vitiated the osteogenesis-promoting effect of naringin.Results:Immunocytochemical staining showed that the cells were Vimentin and Six2(+),E-cadherin and CK-18(−).Naringin can activate the p38 signaling pathway to enhance the osteogenesis of porcine MMCs by increasing cell viability,ALP activity,the expressions of Runx2,OPN and OCN,and the formation of mineralized nodules(P<0.05).The application of p38 signaling pathway inhibitor SB203580 vitiated the osteogenesis-promoting effect of naringin,manifested by decreased ALP activity,the expressions of Runx2,OPN and OCN,and the formation of mineralized nodules(P<0.05).Conclusion:Naringin,the active ingredient of Chinese herbal medicine Rhizoma Drynariae for nourishing Shen(Kidney)and strengthening bone,enhances the osteogenic differentiation of renal MMCs through the p38 signaling pathway.展开更多
文摘目的:通过对单中心大样本慢性乙型肝炎病毒(hepatitis B virus,HBV)感染队列的分析,对我国慢性HBV感染自然病程的划分提出修订建议。方法:回顾性地纳入2014年1月至2020年10月在中国人民解放军总医院第五医学中心接受过肝组织活检的慢性HBV感染者。参考《欧洲肝病学会乙型肝炎病毒感染管理临床实践指南(2017年版)》等国内外最新版慢性乙型肝炎(chronic hepatitis B,CHB)防治指南,将患者按乙型肝炎e抗原(hepatitis B e antigen,HBeAg)状态及肝损伤程度分为HBeAg阳性感染(免疫耐受期)、HBeAg阳性CHB(免疫清除期)、HBeAg阴性感染(免疫控制期)和HBeAg阴性CHB(再活动期)四个自然病程分期,并重点比较了不同分期患者的人口学和实验室检验结果。两组间年龄差异采用Mann-Whitney U检验。结果:最终纳入符合纳排标准的患者760例,包括197例未成年(年龄<18岁)和563例成年感染者,男性456例、女性304例,纳入患者的中位年龄为29岁,(四分位间距:16,39岁)。上述四个自然病程分期患者分别有173、329、95和163例,进一步比较四期患者的年龄发现:HBeAg阴性CHB的中位年龄尽管大于HBeAg阳性CHB(37岁vs.24岁,P<0.001),但却小于HBeAg阴性感染者(37岁vs.39岁,P=0.240)。结论:根据本研究可以推测,HBeAg阴性CHB患者并非都是由HBeAg阴性感染者进入再活动期发展而来,也可以由仍处于持续免疫活动状态的HBeAg阳性CHB患者发生HBeAg阴转或血清学转换而来。
基金Supported by the National Natural Science Foundation of China(Nos.82174115 and 81774027)。
文摘Objective:To explore the potential of metanephric mesenchymal cells(MMCs)for osteogenesis and naringin’s ability to enhance this process and its molecular mechanism.Methods:Porcine MMCs at 70 days of gestation were used as tool cells,cultured in osteogenic induction medium,identified by immunocytochemistry staining.Osteogenic potential of porcine MMCs and naringin’s ability to enhance this process was tested by detecting changes in cell viability,alkaline phosphatase(ALP)activity,the expression of runt-related transcription factor 2(Runx2),osteopontin(OPN)and osteocalcin(OCN),and the formation of mineralized nodules,and the application of the p38 signaling pathway inhibitor SB203580 vitiated the osteogenesis-promoting effect of naringin.Results:Immunocytochemical staining showed that the cells were Vimentin and Six2(+),E-cadherin and CK-18(−).Naringin can activate the p38 signaling pathway to enhance the osteogenesis of porcine MMCs by increasing cell viability,ALP activity,the expressions of Runx2,OPN and OCN,and the formation of mineralized nodules(P<0.05).The application of p38 signaling pathway inhibitor SB203580 vitiated the osteogenesis-promoting effect of naringin,manifested by decreased ALP activity,the expressions of Runx2,OPN and OCN,and the formation of mineralized nodules(P<0.05).Conclusion:Naringin,the active ingredient of Chinese herbal medicine Rhizoma Drynariae for nourishing Shen(Kidney)and strengthening bone,enhances the osteogenic differentiation of renal MMCs through the p38 signaling pathway.
