BACKGROUND: Early diagnosis of liver metastasis of colorectal carcinoma is very important for the appropriate treatment of such patients. However, there has been no effective approach available for clinical applicatio...BACKGROUND: Early diagnosis of liver metastasis of colorectal carcinoma is very important for the appropriate treatment of such patients. However, there has been no effective approach available for clinical application. The present study aimed to investigate the differential expression of proteins in patients with liver metastasis of colorectal carcinomas using proteomic analysis and evaluate its potentiality in clinical diagnosis. METHODS: Fluorescence two-dimensional differential in-gel electrophoresis (2-D DIGE) was used to analyze and compare the protein expression between normal mucosa, the primary focus, and liver metastases. Proteomic analysis was made to identify the differentially expressed proteins. Immunohistological staining was used to confirm the expression of differentially expressed proteins in colorectal carcinomas and areas of liver metastasis. RESULTS: A 1.5-fold difference was found with 46 differentially expressed proteins. In 20 differentially expressed proteins, 3 were down-regulated and 17 up-regulated in liver metastases. Proteomic analysis showed that the S-adenosylmethionine transgelin variant was down-regulated in liver metastasis tissues. Zinc finger protein 64 homolog (Zfp64), guanine nucleotide exchange factor 4 (GEF4), human arginase, glutathione S-transferases (GSTs) A3, and tumor necrosis factor a (TNF-alpha)-induced protein 9 were up-regulated in liver metastasis tissues. Immunohistochemical staining confirmed that human arginase expression was higher in liver metastases than in the primary focus. CONCLUSIONS: There was a significant difference in protein expression between the primary focus of colorectal carcinoma and liver metastases. The differentially regulated proteins were closely related to liver metastasis of colorectal carcinoma. Elevated human arginase may be an important molecular marker for liver metastasis from colorectal carcinoma. (Hepatobiliary Pancreat Dis Jut 2010; 9: 149-153)展开更多
A method of neural network identifying tau decay modes is described.The systematic errors re-lated to the neural network are discussed in detail.A method is used to analyze one-prong hadronic tau de-cays in L3 experim...A method of neural network identifying tau decay modes is described.The systematic errors re-lated to the neural network are discussed in detail.A method is used to analyze one-prong hadronic tau de-cays in L3 experiment.展开更多
Digital image measurement method, as an extension of Particle Image Velocimetry of single-phase flow measurement, was investigated for application to air-water two-phase flows. The method has strong potential ability ...Digital image measurement method, as an extension of Particle Image Velocimetry of single-phase flow measurement, was investigated for application to air-water two-phase flows. The method has strong potential ability in measuring bubble geometrical features and moving velocities for complex bubble motion in aerated water flow. Both diluted and dense bubble rising flows are measured using the digital image method. Measured bubble shapes and sizes, and bubble velocities are affected by threshold selection for binary image. Several algorithms for selecting threshold are compared and methods for calculating the time-averaged void fraction are discussed.展开更多
基金supported by grants from the National Natural Science Foundation of China(No.30471700)the "Tenth one five"Science and Technique Foundation of the PLA,China(No.06G027)
文摘BACKGROUND: Early diagnosis of liver metastasis of colorectal carcinoma is very important for the appropriate treatment of such patients. However, there has been no effective approach available for clinical application. The present study aimed to investigate the differential expression of proteins in patients with liver metastasis of colorectal carcinomas using proteomic analysis and evaluate its potentiality in clinical diagnosis. METHODS: Fluorescence two-dimensional differential in-gel electrophoresis (2-D DIGE) was used to analyze and compare the protein expression between normal mucosa, the primary focus, and liver metastases. Proteomic analysis was made to identify the differentially expressed proteins. Immunohistological staining was used to confirm the expression of differentially expressed proteins in colorectal carcinomas and areas of liver metastasis. RESULTS: A 1.5-fold difference was found with 46 differentially expressed proteins. In 20 differentially expressed proteins, 3 were down-regulated and 17 up-regulated in liver metastases. Proteomic analysis showed that the S-adenosylmethionine transgelin variant was down-regulated in liver metastasis tissues. Zinc finger protein 64 homolog (Zfp64), guanine nucleotide exchange factor 4 (GEF4), human arginase, glutathione S-transferases (GSTs) A3, and tumor necrosis factor a (TNF-alpha)-induced protein 9 were up-regulated in liver metastasis tissues. Immunohistochemical staining confirmed that human arginase expression was higher in liver metastases than in the primary focus. CONCLUSIONS: There was a significant difference in protein expression between the primary focus of colorectal carcinoma and liver metastases. The differentially regulated proteins were closely related to liver metastasis of colorectal carcinoma. Elevated human arginase may be an important molecular marker for liver metastasis from colorectal carcinoma. (Hepatobiliary Pancreat Dis Jut 2010; 9: 149-153)
基金The project supported by the National Natural Science Foundation of China
文摘A method of neural network identifying tau decay modes is described.The systematic errors re-lated to the neural network are discussed in detail.A method is used to analyze one-prong hadronic tau de-cays in L3 experiment.
文摘Digital image measurement method, as an extension of Particle Image Velocimetry of single-phase flow measurement, was investigated for application to air-water two-phase flows. The method has strong potential ability in measuring bubble geometrical features and moving velocities for complex bubble motion in aerated water flow. Both diluted and dense bubble rising flows are measured using the digital image method. Measured bubble shapes and sizes, and bubble velocities are affected by threshold selection for binary image. Several algorithms for selecting threshold are compared and methods for calculating the time-averaged void fraction are discussed.
