Electropolymerized o-phenylenediamine firm is used as a functional coating for the immobilization of anti-S. aureus antibody on the surface of a gold-plated piezoelectric crystal, and this piezoelectric immunosensor i...Electropolymerized o-phenylenediamine firm is used as a functional coating for the immobilization of anti-S. aureus antibody on the surface of a gold-plated piezoelectric crystal, and this piezoelectric immunosensor is applied to detect S. aureus. The frequency shift (Delta F = F-20s - F-380s, Hz) between the frequency at the 20th second (after the addition of sample, F-20s) and that at 380 seconds later (F-380s) was introduced to construct a calibration graph, and shortening of assay time was achieved. The S. aureus concentrations in the range of 10(5)-10(9) cells/mL can be detected by this system.展开更多
基金Financial supports by the National Natural Science Foundation of China (Grant number: 29872003) and by the Scientific Research Foundation for the Returned Overseas Chinese Scholars State Education Ministry are greatly acknowledged.
基金Project supported by the National Natural Science Foundation of China and the State Education commission of China.
文摘Electropolymerized o-phenylenediamine firm is used as a functional coating for the immobilization of anti-S. aureus antibody on the surface of a gold-plated piezoelectric crystal, and this piezoelectric immunosensor is applied to detect S. aureus. The frequency shift (Delta F = F-20s - F-380s, Hz) between the frequency at the 20th second (after the addition of sample, F-20s) and that at 380 seconds later (F-380s) was introduced to construct a calibration graph, and shortening of assay time was achieved. The S. aureus concentrations in the range of 10(5)-10(9) cells/mL can be detected by this system.