Objective:To examine the effects of Astragalus polysaccharide on immune function in B16 melanoma mice.Method:Forty male C57BL/6 mice were divided equally into a control group,model group,Astragalus polysaccharide low-...Objective:To examine the effects of Astragalus polysaccharide on immune function in B16 melanoma mice.Method:Forty male C57BL/6 mice were divided equally into a control group,model group,Astragalus polysaccharide low-dose group,and Astragalus polysaccharide high-dose group,with 10 mice per group.B16 cells were used to develop a mouse model of melanoma.After B16 engraftment,40 mg/kg and 80 mg/kg Astragalus polysaccharide was administered by gavage every day to the Astragalus polysaccharide low-dose group and Astragalus polysaccharide high-dose group,respectively.Splenic index,thymic index,tumor growth curves,and tumor inhibition rates were measured.Flow cytometry was used to measure proportions of peripheral blood T lymphocyte subsets.Hematoxylin and eosin staining was used to examine histopathological changes in tumors.Immunofluorescence double staining was used to identify myeloid-derived suppressor cells in tumor tissues.Results:In the Astragalus polysaccharide high-dose group,splenic and thymic indices were significantly increased and tumor growth was inhibited in melanoma mice.Flow cytometry demonstrated increased CD4^(+)and CD4^(+)/CD8^(+)T-cell ratios in the high-dose Astragalus polysaccharide group.HE staining demonstrated significantly decreased numbers of tumor cells among mice with melanoma in the high-dose Astragalus polysaccharide group.Immunofluorescence double staining demonstrated significantly decreased numbers of myeloid-derived suppressor cells in tumor tissues in the high-dose Astragalus polysaccharide group.Conclusion:Astragalus polysaccharide inhibits tumor growth,increases splenic and thymic indices,increases CD4^(+)and CD4^(+)/CD8^(+)T-cell ratios,and decreases myeloid-derived suppressor cell numbers in melanoma mice.Our results indicate Astragalus polysaccharide may enhance immune function resulting in inhibition of tumorigenesis and tumor progression.展开更多
文摘Objective:To examine the effects of Astragalus polysaccharide on immune function in B16 melanoma mice.Method:Forty male C57BL/6 mice were divided equally into a control group,model group,Astragalus polysaccharide low-dose group,and Astragalus polysaccharide high-dose group,with 10 mice per group.B16 cells were used to develop a mouse model of melanoma.After B16 engraftment,40 mg/kg and 80 mg/kg Astragalus polysaccharide was administered by gavage every day to the Astragalus polysaccharide low-dose group and Astragalus polysaccharide high-dose group,respectively.Splenic index,thymic index,tumor growth curves,and tumor inhibition rates were measured.Flow cytometry was used to measure proportions of peripheral blood T lymphocyte subsets.Hematoxylin and eosin staining was used to examine histopathological changes in tumors.Immunofluorescence double staining was used to identify myeloid-derived suppressor cells in tumor tissues.Results:In the Astragalus polysaccharide high-dose group,splenic and thymic indices were significantly increased and tumor growth was inhibited in melanoma mice.Flow cytometry demonstrated increased CD4^(+)and CD4^(+)/CD8^(+)T-cell ratios in the high-dose Astragalus polysaccharide group.HE staining demonstrated significantly decreased numbers of tumor cells among mice with melanoma in the high-dose Astragalus polysaccharide group.Immunofluorescence double staining demonstrated significantly decreased numbers of myeloid-derived suppressor cells in tumor tissues in the high-dose Astragalus polysaccharide group.Conclusion:Astragalus polysaccharide inhibits tumor growth,increases splenic and thymic indices,increases CD4^(+)and CD4^(+)/CD8^(+)T-cell ratios,and decreases myeloid-derived suppressor cell numbers in melanoma mice.Our results indicate Astragalus polysaccharide may enhance immune function resulting in inhibition of tumorigenesis and tumor progression.
