反转录环介导等温扩增技术快速检测猪水疱病毒方法的建立

为了建立一种针对猪水泡病毒(SVDV)的反转录环介导等温扩增技术(RT-LAMP),用于减少猪水泡病与口蹄疫因临床症状相似而造成的误诊,试验针对SVDV VP1基因序列设计4个LAMP引物,其中外侧引物用于SVDV PCR检测,并利用RT-LAMP和RT-PCR方法进行敏感性试验、特异性试验以及临床样品检测。结果表明:针对SVDV的RT-LAMP检测方法的敏感性是RT-PCR的10倍;RT-LAMP方法的特异性良好,与口蹄疫病毒无交叉反应;两种方法对临床样品检测的符合率为100%。说明试验建立的RT-LAMP方法具有敏感性高、特异性强、快速等特点,适合实验室和临床对SVDV的快速诊断。

Modeling Study of a New Circulating Fluidized Bi-BedBoiler Combustion System

This paper presents a set of general dynamic mathematical models for the combustion system of acirculating fluidized bi-bed boiler. The models fully consider the flow, combustion and heat transfercharacteristics, and describe the physical and chemical processes inside the bi-bed, including the gassolid flow, multiple particles combustion, gas chemical reactions, heat transfer and pressure balances,etc.