目的:通过四氯化碳(CCl_(4))和乙醇综合法建立联合门静脉离断与肝脏离断的二步肝切除术(associating liver partition and portal vein ligation for staged hepatectomy,ALPPS)的肝纤维化模型,探讨ALPPS应用于肝纤维化大鼠的安全性及...目的:通过四氯化碳(CCl_(4))和乙醇综合法建立联合门静脉离断与肝脏离断的二步肝切除术(associating liver partition and portal vein ligation for staged hepatectomy,ALPPS)的肝纤维化模型,探讨ALPPS应用于肝纤维化大鼠的安全性及可行性。方法:将40只雄性SD大鼠采用抽签法随机均分为对照组和肝纤维化组,每组各20只。对照组大鼠进行常规饲养,并采用橄榄油注射4周。肝纤维化组大鼠采用40%CCl_(4)的橄榄油溶液背部皮下注射+饮用10%乙醇4周。肝纤维化组和对照组各取5只大鼠,进行天狼猩红染色判断肝纤维化的程度。其余15只大鼠行ALPPS,并于术后即刻、第3天、第6天各取5只大鼠,取再生肝脏称重,检测肝脏增生情况,并进一步通过Ki-67免疫组织化学染色判断肝细胞再生情况。结果:通过CCl_(4)和乙醇综合法建模4周,肝纤维化组大鼠体重缓慢增加,生活基本未受影响,且肝纤维化均匀,有1只大鼠因注射CCl_(4)死亡,建模成功率为95.0%。肝纤维化组和对照组大鼠在ALPPS后均实现了剩余肝脏体积的快速增长,且两组差异无统计学意义(P>0.05)。肝纤维化组和对照组大鼠肝组织的Ki-67阳性细胞均在术后第3天明显增多,术后第6天明显减少。结论:采用CCl_(4)背部皮下注射和乙醇综合法可以快速建立肝纤维化大鼠模型,在此基础上进行ALPPS是安全可行的。本研究建立了稳定高效的肝纤维化大鼠ALPPS模型。展开更多
Methylene blue(MB)was reported to have neuronal protective effect after brain ischermia.In this study,we aimed to investigate the effect of MB on white matter in rats after focal cerebral ischemia-reperfusion(MCAO/R)i...Methylene blue(MB)was reported to have neuronal protective effect after brain ischermia.In this study,we aimed to investigate the effect of MB on white matter in rats after focal cerebral ischemia-reperfusion(MCAO/R)injury.MCAO/R model was set and 54 male SD rats were randomly divided into 3 groups:control(sham surgery group),middle cerebral artery occlusion and reperfusion(MCAO),MCAO treated with MB of 10 mg/kg(MB)by intraperitoneal injection 1.5 h after surgery.We observed the neurological deficits in different groups using foot fault test 24 h after MCAO.We measured the infarction volume using TTC staining and showed the morphological changes of neurons and myelin using Nissl and black gold staining.展开更多
This project intends to deploy image information such as magnetic resonance imaging(MRI)and digital subtraction angiography(DSA)com bined with stereo positioning system to scan,display and locate anatomical structures...This project intends to deploy image information such as magnetic resonance imaging(MRI)and digital subtraction angiography(DSA)com bined with stereo positioning system to scan,display and locate anatomical structures such as bones and joints in corpses and living bodies.We employ 3D point clouds as efficient representations of MRI,and propose point clouds denoising and inpainting leveraging on the field of graph signal processing.Virtual reality surgery for specific patients,such as arthroplasty,can be performed multiple times in the ward,and the calculated force is communicated to the operator through the force feedback device to obtain a feel that is close to the actual operation.展开更多
文摘目的:通过四氯化碳(CCl_(4))和乙醇综合法建立联合门静脉离断与肝脏离断的二步肝切除术(associating liver partition and portal vein ligation for staged hepatectomy,ALPPS)的肝纤维化模型,探讨ALPPS应用于肝纤维化大鼠的安全性及可行性。方法:将40只雄性SD大鼠采用抽签法随机均分为对照组和肝纤维化组,每组各20只。对照组大鼠进行常规饲养,并采用橄榄油注射4周。肝纤维化组大鼠采用40%CCl_(4)的橄榄油溶液背部皮下注射+饮用10%乙醇4周。肝纤维化组和对照组各取5只大鼠,进行天狼猩红染色判断肝纤维化的程度。其余15只大鼠行ALPPS,并于术后即刻、第3天、第6天各取5只大鼠,取再生肝脏称重,检测肝脏增生情况,并进一步通过Ki-67免疫组织化学染色判断肝细胞再生情况。结果:通过CCl_(4)和乙醇综合法建模4周,肝纤维化组大鼠体重缓慢增加,生活基本未受影响,且肝纤维化均匀,有1只大鼠因注射CCl_(4)死亡,建模成功率为95.0%。肝纤维化组和对照组大鼠在ALPPS后均实现了剩余肝脏体积的快速增长,且两组差异无统计学意义(P>0.05)。肝纤维化组和对照组大鼠肝组织的Ki-67阳性细胞均在术后第3天明显增多,术后第6天明显减少。结论:采用CCl_(4)背部皮下注射和乙醇综合法可以快速建立肝纤维化大鼠模型,在此基础上进行ALPPS是安全可行的。本研究建立了稳定高效的肝纤维化大鼠ALPPS模型。
文摘Methylene blue(MB)was reported to have neuronal protective effect after brain ischermia.In this study,we aimed to investigate the effect of MB on white matter in rats after focal cerebral ischemia-reperfusion(MCAO/R)injury.MCAO/R model was set and 54 male SD rats were randomly divided into 3 groups:control(sham surgery group),middle cerebral artery occlusion and reperfusion(MCAO),MCAO treated with MB of 10 mg/kg(MB)by intraperitoneal injection 1.5 h after surgery.We observed the neurological deficits in different groups using foot fault test 24 h after MCAO.We measured the infarction volume using TTC staining and showed the morphological changes of neurons and myelin using Nissl and black gold staining.
文摘This project intends to deploy image information such as magnetic resonance imaging(MRI)and digital subtraction angiography(DSA)com bined with stereo positioning system to scan,display and locate anatomical structures such as bones and joints in corpses and living bodies.We employ 3D point clouds as efficient representations of MRI,and propose point clouds denoising and inpainting leveraging on the field of graph signal processing.Virtual reality surgery for specific patients,such as arthroplasty,can be performed multiple times in the ward,and the calculated force is communicated to the operator through the force feedback device to obtain a feel that is close to the actual operation.
