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非酒精性脂肪性肝病合并2型糖尿病大鼠模型的建立 被引量:1
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作者 柯淑红 郑承红 +2 位作者 彭聪 周扬 马威 《世界华人消化杂志》 CAS 2018年第31期1805-1811,共7页
目的诱导非酒精性脂肪性肝病(non-alcoholic fatty liver disease, NAFLD)合并2型糖尿病(type 2 diabetes,T2DM)大鼠模型.方法高脂高糖饮食结合小剂量链脲佐菌素(streptozotocin,STZ)腹腔注射建立非酒精性脂肪肝T2DM-大鼠模型. 70只无... 目的诱导非酒精性脂肪性肝病(non-alcoholic fatty liver disease, NAFLD)合并2型糖尿病(type 2 diabetes,T2DM)大鼠模型.方法高脂高糖饮食结合小剂量链脲佐菌素(streptozotocin,STZ)腹腔注射建立非酒精性脂肪肝T2DM-大鼠模型. 70只无特定病原体(specific pathogen free, SPF)级200-220g雌性Wistar大鼠随机分为2组:正常对照组(普通饮食喂养, n=15)及模型组(高糖高脂喂养后腹腔注射30 mg/kg STZ, n=55).比较喂养4 wk、7 wk时,各组大鼠饮食量、饮水量、体重、葡萄糖耐量试验(oral glucose tolerance test, OGTT)及其曲线下面积(area under the curve, AUC)变化,比较喂养7 wk时各组大鼠血清甘油三酯(triglyceride, TG)、血清总胆固醇(total cholesterol, TC)、谷丙转氨酶(alanine aminotransferase, ALT)、谷草转氨酶(glutamicoxalacetictransaminase, AST)、Ⅲ型前胶原氨端肽(procollagenⅢamino-terminalpeptide,PⅢNP)及透明质酸(hyal-uronic acid, HA)、胰岛素(fasting serum insulin,FINs)、胰岛素抵抗指数(insulinresistence index,HOMA-IR)水平变化,并于喂养2wk、4wk和7 wk时观察各组大鼠肝脏病理结构的改变.结果喂养7 wk时,与正常对照组相比,模型组大鼠OGTT及其AUC、体重、饮食量、饮水量、TG、TC、FINs、HOMA-IR、ALT、AST、PⅢNP、HA明显高升高有显著统计学差异(P<0.01),随着造模时间的延长,光镜下肝细胞由单纯脂肪变性加重,最终出现大泡型或以大泡为主的混合性肝细胞脂肪变,胶原蛋白明显增多,为典型NAFLD病理改变.结论小剂量腹腔注射STZ并高糖高脂饲料喂养可诱导稳定的NAFLD T2DM大鼠模型. 展开更多
关键词 非酒精性脂肪性肝病 链脲佐菌素 高糖高脂饮食 2型糖尿病
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Effect of Jianpi Liqi recipe on diabetic dyspepsia and its effect on blood lipid level
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作者 Zhen Mao cheng-hong zheng +1 位作者 Run Jiang Pai-Di Xu 《Journal of Hainan Medical University》 2021年第14期30-36,共7页
Objective:To observe the clinical effect of Jianpi Liqi Decoction on diabetic gastroparesis.Methods:80 patients were randomly divided into 2 groups,Treatment and control groups,40 cases per group,The treatment group u... Objective:To observe the clinical effect of Jianpi Liqi Decoction on diabetic gastroparesis.Methods:80 patients were randomly divided into 2 groups,Treatment and control groups,40 cases per group,The treatment group used Jianpi Liqi recipe,One dose a day,Take it twice,Six times a week,For 4 weeks;Control group with mosapride citrate,5 mg,each Three times a day,Take continuously for 4 weeks.Observe the two groups before treatment,2 weeks after treatment,4 weeks after treatment,Subjective symptoms(early satiety,abdominal distention,upper abdomen burning,upper abdomen pain)score;Before and after treatment,The contents of FBG,2 hPG,HbA1c,TC,GAS,MLT in the two groups.Results:The scores of early satiety,abdominal distention,burning sensation of upper abdomen and pain of upper abdomen were decreased,Intra-group comparisons,Differences were statistically significant(P<0.05);After treatment,the gastric emptying rate increased in both groups,A more significant increase in the treatment group(P<0.05);Treatment group after treatment TC,TG,LDL-C decreased more significantly(P<0.05);After treatment,the GAS,MLT content of both groups increased significantly,Intergroup comparisons,and the difference was statistically significant(P<0.05).Conclusion:Jianpi Liqi Decoction is effective in treating Diabetic gastroparesis,High security,it is worth clinical use. 展开更多
关键词 Diabetic dyspepsia Jianpi Liqi prescription Clinical observation Randomized controlled
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