SRY-related HMG-box gene 2(SOX2)is one of the key regulatory genes that maintain the pluripotency and self-renewal properties in embryonic stem cells.Here we used immunohistochemistry to analyze the expression of SOX2...SRY-related HMG-box gene 2(SOX2)is one of the key regulatory genes that maintain the pluripotency and self-renewal properties in embryonic stem cells.Here we used immunohistochemistry to analyze the expression of SOX2 in human prostate tissues and found it contributed to tumorigenesis and correlated with histologic grade and Gleason score.We further investigated SOX2’s function in cell growth and apoptosis process by using a human prostate cancer cell line DU145 with SOX2 overexpression or down-regulation.Cell cycle assay revealed that SOX2 promoted cell growth and increased the percentage of cells in S phase.In vitro and in vivo xenograft experiments in NOD/SCID mice further demonstrated that SOX2 increased the apoptosis-resistant properties of DU145 cells with decreased function of store-operated Ca21 entry and reduced expression of Orai1 at both mRNA and protein levels,suggesting a potential mechanism that contributes to the anti-apoptotic property of SOX2.To our knowledge,this study is the first to investigate SOX2’s function in tumorigenesis and apoptosis of human prostate cancer and to elucidate its regulatory effect on the activity of store-operated Ca21 channels.Our results support the concept that SOX2 has the potential to be a significant marker to evaluate the progression of prostate cancer and serve as a potentially useful target for prostate cancer therapy.展开更多
基金funded by the National Science Foundation of China(30830096 to R.X.,31000616 to N.L.)the Major State Basic Research Development Program of China(973 Program)(2007CB914804 to R.X.)+2 种基金Key Project of Tianjin Scientific&Technological Commission for China-Sweden Cooperation Research Program(09ZCZDSF04000 to R.X.)China Junior Faculty Fund(20090031120044 to N.L.)China Fundamental Research Fund for the Central Universities(65011241 to N.L.).
文摘SRY-related HMG-box gene 2(SOX2)is one of the key regulatory genes that maintain the pluripotency and self-renewal properties in embryonic stem cells.Here we used immunohistochemistry to analyze the expression of SOX2 in human prostate tissues and found it contributed to tumorigenesis and correlated with histologic grade and Gleason score.We further investigated SOX2’s function in cell growth and apoptosis process by using a human prostate cancer cell line DU145 with SOX2 overexpression or down-regulation.Cell cycle assay revealed that SOX2 promoted cell growth and increased the percentage of cells in S phase.In vitro and in vivo xenograft experiments in NOD/SCID mice further demonstrated that SOX2 increased the apoptosis-resistant properties of DU145 cells with decreased function of store-operated Ca21 entry and reduced expression of Orai1 at both mRNA and protein levels,suggesting a potential mechanism that contributes to the anti-apoptotic property of SOX2.To our knowledge,this study is the first to investigate SOX2’s function in tumorigenesis and apoptosis of human prostate cancer and to elucidate its regulatory effect on the activity of store-operated Ca21 channels.Our results support the concept that SOX2 has the potential to be a significant marker to evaluate the progression of prostate cancer and serve as a potentially useful target for prostate cancer therapy.
