OR2W3在人胰腺癌中的表达及临床意义

目的探讨嗅觉受体家族2亚家族W成员3(olfactory receptor family 2 subfamily W member,OR2W3)在胰腺癌(pancreatic cancer,PC)组织中的表达及其临床意义.方法采用免疫组织化学法检测50例PC组织及癌旁组织石蜡切片中的OR2W3的表达,分析OR2W3蛋白表达与临床病理因素的关系.结果 OR2W3蛋白在PC组织中高表达和低表达率分别为78.0%(39/50)和22.0%(11/50),而在癌旁组织中高表达和低表达率分别为12.0%(6/50)和为88.0%(44/50),两者差异有统计学意义(χ2=44.00,P<0.05).单因素分析显示OR2W3表达与PC的组织分化程度、临床分期、淋巴结转移密切相关,该3因素分组间的差异均有统计学意义(均P<0.05).结论 OR2W3的高表达可能在PC发生、发展中起重要作用.

CD133^+ gallbladder carcinoma cells exhibit self-renewal ability and tumorigenicity

AIM:To identify cancer stem cells(CSCs) in human gallbladder carcinomas(GBCs).METHODS:Primary GBC cells were cultured under serum-free conditions to produce floating spheres.The stem-cell properties of the sphere-forming cells,including self-renewal,differentiation potential,chemoresistance and tumorigenicity,were determined in vitro or in vivo.Cell surface expression of CD133 was investigated in primary tumors and in spheroid cells using flow cytometry.The sphere-colony-formation ability and tumorigenicity of CD133+ cells were assayed.RESULTS:In vitro culture experiments revealed thatfloating spheroids were generated from primary GBC cells,and these sphere-forming cells could generate new progeny spheroids in serum-free media.Spheroid cells were differentiated under serum-containing conditions with downregulation of the stem cell markers Oct-4,Nanog,and nestin(P < 0.05).The differentiated cells showed lower spheroid-colony-formation ability than the original spheroid cells(P < 0.05).Spheroid cells were more resistant to chemotherapeutic reagents than the congenetic adherent cells(P < 0.05).Flow cytometry showed enriched CD133+ population in sphereforming cells(P < 0.05).CD133+ cells possessed high colony-formation ability than the CD133-population(P < 0.01).CD133+ cells injected into nude mice revealed higher tumorigenicity than their antigen-negative counterparts(P < 0.05).CONCLUSION:CD133 may be a cell surface marker for CSCs in GBC.

Wee1抑制剂MK-1775对GBC-SD细胞系胆囊癌干细胞样细胞自我更新的影响

目的 研究Wee1抑制剂MK-1775对GBC-SD细胞系胆囊癌干细胞样细胞自我更新的抑制作用.方法 体外培养GBC-SD细胞系,在无血清干细胞培养基中加入MK-1775后培养悬浮肿瘤干细胞球; Westernblot检测Wee1的表达,并分析比较肿瘤干细胞球体积大小和形成率的变化;建立裸鼠皮下移植瘤模型后MK-17751灌胃2 wk, 2 wk后检测分析移植瘤的重量.结果 加入MK-1775培养8 d后, GBC-SD细胞中Wee1表达下调,同时肿瘤干细胞球大小及形成率均受到抑制;经MK-17751处理后,裸鼠皮下移植瘤生长受到抑制.结论 MK-1775具有抑制GBC-SD细胞系胆囊癌干细胞样细胞自我更新的作用.

A new approach for Roux-en-Y reconstruction after pancreaticoduodenectomy

BACKGROUND： Postoperative pancreatic fistula remains the most common complication of pancreaticoduodenectomy （PD） and is potentially lethal. It contributes significantly to prolonged hospitalization and mortality. In this study, we introduced a new technical approach, a modified Roux-en-Y reconstruction and evaluated its safety and feasibility. METHODS： We retrospectively reviewed the patients who had undergone PD with the modified Roux-en-Y reconstructive technique for periampullary malignancies from January 2011 to June 2012. The data on complications, hospital stay and outcomes after the modified Roux-en-Y reconstruction were analyzed. RESULTS： The reconstruction was performed in 171 patients, of whom 92 received pancreaticogastrostomy and 79 received pancreaticojejunostomy. The median duration of surgery was 4.0 hours （range 3.1-6.9） in all patients, and the median blood loss was 530 mL （range 200-2000）. Sixty-nine patients were subjected to transfusions, with a median transfusion volume of 430 mL （range 200-1400）. The median hospital stay of the patients was 14 days （range 11-38）. Their operative mortality was zero and overall morbidity was 18.1% （31 patients）. Only four patients （2.3%） developed pancreatic fistulas （grade A fistulas in two patients and grade B in two patients）; no patients developed grade C fistula. None of the patients developed bile reflux gastritis. CONCLUSIONS： The modified Roux-en-Y reconstruction, which isolates biliary anastomosis from pancreatic, gastric or jejunalanastomosis, is a safe, reliable, and favorable technique. But it needs further investigation in randomized controlled trials.