BACKGROUND The FreeStyle Libre flash glucose monitoring(FGM)system entered the Chinese market in 2017 to complement the self-monitoring of blood glucose.Due to its increased usage in clinics,the number of studies inve...BACKGROUND The FreeStyle Libre flash glucose monitoring(FGM)system entered the Chinese market in 2017 to complement the self-monitoring of blood glucose.Due to its increased usage in clinics,the number of studies investigating its accuracy has increased.However,its accuracy has not been investigated in highland populations in China.AIM To evaluate measurements recorded using the FreeStyle Libre FGM system compared with capillary blood glucose measured using the enzyme electrode method in patients with type 2 diabetes(T2D)who had migrated within 3 mo from highlands to plains.METHODS Overall,68 patients with T2D,selected from those who had recently migrated from highlands to plains(within 3 mo),were hospitalized at the Department of Endocrinology from August to October 2017 and underwent continuous glucose monitoring(CGM)with the FreeStyle Libre FGM system for 14 d.Throughout the study period,fingertip capillary blood glucose was measured daily using the enzyme electrode method(Super GL,China),and blood glucose levels were read from the scanning probe during fasting and 2 h after all three meals.Moreover,the time interval between reading the data from the scanning probe and collecting fingertip capillary blood was controlled to<5 min.The accuracy of the FGM system was evaluated according to the CGM guidelines.Subsequently,the factors influencing the mean absolute relative difference(MARD)of this system were analyzed by a multiple linear regression method.RESULTS Pearson’s correlation analysis showed that the fingertip and scanned glucose levels were positively correlated(R=0.86,P=0.00).The aggregated MARD of scanned glucose was 14.28±13.40%.Parker's error analysis showed that 99.30%of the data pairs were located in areas A and B.According to the probe wear time of the FreeStyle Libre FGM system,MARD_(1 d) and MARD_(2-14 d) were 16.55%and 14.35%,respectively(t=1.23,P=0.22).Multiple stepwise regression analysis showed that MARD did not correlate with blood glucose when the largest amplitude of glycemic excursion(LAGE)was<5.80 mmol/L but negatively correlated with blood glucose when the LAGE was≥5.80 mmol/L.CONCLUSION The FreeStyle Libre FGM system has good accuracy in patients with T2D who had recently migrated from highlands to plains.This system might be ideal for avoiding the effects of high hematocrit on blood glucose monitoring in populations that recently migrated to plains.MARD is mainly influenced by glucose levels and fluctuations,and the accuracy of the system is higher when the blood glucose fluctuation is small.In case of higher blood glucose level fluctuations,deviation in the scanned glucose levels is the highest at extremely low blood glucose levels.展开更多
A label-free"light-on"colorimetric assay for ascorbic acid(AA)determination is unprecedentedly devised by regulating the intrinsic peroxidase-mimetic activity of the Fe-MIL-88.AA can reduce Fe(Ⅲ)presented i...A label-free"light-on"colorimetric assay for ascorbic acid(AA)determination is unprecedentedly devised by regulating the intrinsic peroxidase-mimetic activity of the Fe-MIL-88.AA can reduce Fe(Ⅲ)presented in Fe-MIL-88 to Fe(Ⅱ),which will dramatically promote the hydroxyl radicals(·OH)conversion efficiency of H_(2)O_(2)through a Fenton reaction,and effectually accelerate the Fe-MIL-88 catalyzed chromogenic reaction of 3,3′,5,5′-tetramethylbenzidine(TMB)-H_(2)O_(2).Redox reaction between AA and H_(2)O_(2)also can produce·OH and promote this chromogenic reaction.Thus,quantification of AA is real-ized via determining the enhanced colorimetric signals.The linear range is 0.5−40μM with a detection limit of 0.12μM.Furthermore,the straightforward yet effective colorimetric strategy with the merits of simplicity,cost-effectiveness,high accuracy,specificity,and sensitivity is widely utilized to determine the concentrations of AA in serological tests.展开更多
基金Supported by Health and Family Planning Project of Sichuan Province,No.17PJ069Tibet Autonomous Region Science and Technology Program,No.XZ202303ZY0011G.
文摘BACKGROUND The FreeStyle Libre flash glucose monitoring(FGM)system entered the Chinese market in 2017 to complement the self-monitoring of blood glucose.Due to its increased usage in clinics,the number of studies investigating its accuracy has increased.However,its accuracy has not been investigated in highland populations in China.AIM To evaluate measurements recorded using the FreeStyle Libre FGM system compared with capillary blood glucose measured using the enzyme electrode method in patients with type 2 diabetes(T2D)who had migrated within 3 mo from highlands to plains.METHODS Overall,68 patients with T2D,selected from those who had recently migrated from highlands to plains(within 3 mo),were hospitalized at the Department of Endocrinology from August to October 2017 and underwent continuous glucose monitoring(CGM)with the FreeStyle Libre FGM system for 14 d.Throughout the study period,fingertip capillary blood glucose was measured daily using the enzyme electrode method(Super GL,China),and blood glucose levels were read from the scanning probe during fasting and 2 h after all three meals.Moreover,the time interval between reading the data from the scanning probe and collecting fingertip capillary blood was controlled to<5 min.The accuracy of the FGM system was evaluated according to the CGM guidelines.Subsequently,the factors influencing the mean absolute relative difference(MARD)of this system were analyzed by a multiple linear regression method.RESULTS Pearson’s correlation analysis showed that the fingertip and scanned glucose levels were positively correlated(R=0.86,P=0.00).The aggregated MARD of scanned glucose was 14.28±13.40%.Parker's error analysis showed that 99.30%of the data pairs were located in areas A and B.According to the probe wear time of the FreeStyle Libre FGM system,MARD_(1 d) and MARD_(2-14 d) were 16.55%and 14.35%,respectively(t=1.23,P=0.22).Multiple stepwise regression analysis showed that MARD did not correlate with blood glucose when the largest amplitude of glycemic excursion(LAGE)was<5.80 mmol/L but negatively correlated with blood glucose when the LAGE was≥5.80 mmol/L.CONCLUSION The FreeStyle Libre FGM system has good accuracy in patients with T2D who had recently migrated from highlands to plains.This system might be ideal for avoiding the effects of high hematocrit on blood glucose monitoring in populations that recently migrated to plains.MARD is mainly influenced by glucose levels and fluctuations,and the accuracy of the system is higher when the blood glucose fluctuation is small.In case of higher blood glucose level fluctuations,deviation in the scanned glucose levels is the highest at extremely low blood glucose levels.
基金supported by the National Natural Science Foundation of China(21904048,21902061 and 21902062)the Natural Science Foundation of Shandong Province(ZR2019YQ10 and ZR2020QB033)the Young Taishan Scholars Program(tsqn201812080).
文摘A label-free"light-on"colorimetric assay for ascorbic acid(AA)determination is unprecedentedly devised by regulating the intrinsic peroxidase-mimetic activity of the Fe-MIL-88.AA can reduce Fe(Ⅲ)presented in Fe-MIL-88 to Fe(Ⅱ),which will dramatically promote the hydroxyl radicals(·OH)conversion efficiency of H_(2)O_(2)through a Fenton reaction,and effectually accelerate the Fe-MIL-88 catalyzed chromogenic reaction of 3,3′,5,5′-tetramethylbenzidine(TMB)-H_(2)O_(2).Redox reaction between AA and H_(2)O_(2)also can produce·OH and promote this chromogenic reaction.Thus,quantification of AA is real-ized via determining the enhanced colorimetric signals.The linear range is 0.5−40μM with a detection limit of 0.12μM.Furthermore,the straightforward yet effective colorimetric strategy with the merits of simplicity,cost-effectiveness,high accuracy,specificity,and sensitivity is widely utilized to determine the concentrations of AA in serological tests.