Macadamia from the family Proteaceae is a plant native to Australia and has long been favoured by people for its crispy and high nutritional and medicinal value.Here,the genome of GUIRE 1(GR1),a highly heterozygous su...Macadamia from the family Proteaceae is a plant native to Australia and has long been favoured by people for its crispy and high nutritional and medicinal value.Here,the genome of GUIRE 1(GR1),a highly heterozygous superior cultivar of macadamia nut,was sequenced and assembled using nanopore sequencing,and a 807-Mb genome(contig N50,1.9 Mb;scaffold N50,54.70 Mb)and 14 chromosomes were obtained.A total of 453 Mb(about 55.95%)repetitive sequences and 37,657 protein-coding genes were obtained by gene annotation and homologous protein comparison.Proteaceae diverged from Nelumbonaceae nearly 115.37 million years ago and from Rubiaceae about 140 million years ago.A genome-wide duplication(WGD)event occurred in macadamia 41 million years ago based on the WGD analysis.The functional enrichment analysis of M.integrifolia-specific gene families revealed their roles in signal transduction,protein phosphorylation,protein binding,and defense response.Here,a highly heterozygous genome of M.integrifolia was unlocked to provide a database for breeding and molecular mechanism research.展开更多
A novel route for tandem C-C/C-N formation,annulation and aromatization of hydrazones with 1,2-dichloroethane to synthesize 1 H-pyrazoles has been developed.Furthermore,the 1,2-dichloroethane serves as alkylation reag...A novel route for tandem C-C/C-N formation,annulation and aromatization of hydrazones with 1,2-dichloroethane to synthesize 1 H-pyrazoles has been developed.Furthermore,the 1,2-dichloroethane serves as alkylation reagent in good to excellent yields.This methodology features mild reaction conditions and good functional group tolerance,providing a direct approach for the preparation of 1 Hpvrazoles.展开更多
基金supported by Guangxi Natural Science Foundation under Grant No.2019GXNSFBA18501.
文摘Macadamia from the family Proteaceae is a plant native to Australia and has long been favoured by people for its crispy and high nutritional and medicinal value.Here,the genome of GUIRE 1(GR1),a highly heterozygous superior cultivar of macadamia nut,was sequenced and assembled using nanopore sequencing,and a 807-Mb genome(contig N50,1.9 Mb;scaffold N50,54.70 Mb)and 14 chromosomes were obtained.A total of 453 Mb(about 55.95%)repetitive sequences and 37,657 protein-coding genes were obtained by gene annotation and homologous protein comparison.Proteaceae diverged from Nelumbonaceae nearly 115.37 million years ago and from Rubiaceae about 140 million years ago.A genome-wide duplication(WGD)event occurred in macadamia 41 million years ago based on the WGD analysis.The functional enrichment analysis of M.integrifolia-specific gene families revealed their roles in signal transduction,protein phosphorylation,protein binding,and defense response.Here,a highly heterozygous genome of M.integrifolia was unlocked to provide a database for breeding and molecular mechanism research.
基金financial support for this research from Shandong Provincial Natural Science Foundation(No.ZR2017LB006)Academic promotion programme of Shandong First Medical University(No.2019LJ003)。
文摘A novel route for tandem C-C/C-N formation,annulation and aromatization of hydrazones with 1,2-dichloroethane to synthesize 1 H-pyrazoles has been developed.Furthermore,the 1,2-dichloroethane serves as alkylation reagent in good to excellent yields.This methodology features mild reaction conditions and good functional group tolerance,providing a direct approach for the preparation of 1 Hpvrazoles.
