Leaf senescence,the final stage of leaf development,is influenced by numerous internal and environmental signals.So far,how biotic stresses such as pathogen infection regulate leaf senescence is unclear.Here,we found ...Leaf senescence,the final stage of leaf development,is influenced by numerous internal and environmental signals.So far,how biotic stresses such as pathogen infection regulate leaf senescence is unclear.Here,we found that the premature leaf senescence caused by a soil-borne vascular fungus Verticillium dahliae in Arabidopsis was impaired by the mutation of a protein elicitor from V.dahliae 1(PevD1).Constitutive or inducible overexpression of PevD1 accelerated Arabidopsis leaf senescence.A senescence-associated NAC transcription factor,ORE1,was targeted by PevD1.PevD1 interacted with and stabilized ORE1 protein by disrupting its interaction with the RING-type ubiquitin E3 ligase NLA.Mutation of ORE1 suppressed the premature senescence caused by overexpressing PevD1.Overexpression of ORE1 or PevD1 led to enhanced ethylene production,and ORE1 mediated PevD1-induced ethylene biosynthesis by directly binding to the ACS6 promoter.Loss-of-function of ACSs suppressed V.dahliae-induced leaf senescence in ORE1-overexpressing plants.Interestingly,PevD1 also interacted with Gossypium hirsutum ORE1(GhORE1),and virus-induced gene silencing of GhORE1 delayed V.dahliae-triggered leaf senescence in cotton,indicative of the existence of a conserved mechanism in plants.Altogether,our study demonstrates that V.dahliae induces leaf senescence by secreting the effector PevD1 to regulate the ORE1-ACS6 cascade,providing new insight into biotic stress-induced senescence in plants.展开更多
基金This work was supported by the National Natural Science Foundation of China(31970196 and 32011540381 to Z.L.,31900173 to H.W.,31770649 to X.X.)the National Key Research and Development Program of China(No.2019YFA0903904 to H.G.)+2 种基金Shenzhen Science and Technology Program(KQTD20190929173906742 to H.G.)China Postdoctoral Science Foundation(2019M650514 to Y.Z.and 2020M670544 to Y.G.,and 2019M650516 to H.W.)the startup funding for plant aging research from"Beijing Advanced Innovation Center for Tree Breeding by Molecular Design,Beijing Forestry University".
文摘Leaf senescence,the final stage of leaf development,is influenced by numerous internal and environmental signals.So far,how biotic stresses such as pathogen infection regulate leaf senescence is unclear.Here,we found that the premature leaf senescence caused by a soil-borne vascular fungus Verticillium dahliae in Arabidopsis was impaired by the mutation of a protein elicitor from V.dahliae 1(PevD1).Constitutive or inducible overexpression of PevD1 accelerated Arabidopsis leaf senescence.A senescence-associated NAC transcription factor,ORE1,was targeted by PevD1.PevD1 interacted with and stabilized ORE1 protein by disrupting its interaction with the RING-type ubiquitin E3 ligase NLA.Mutation of ORE1 suppressed the premature senescence caused by overexpressing PevD1.Overexpression of ORE1 or PevD1 led to enhanced ethylene production,and ORE1 mediated PevD1-induced ethylene biosynthesis by directly binding to the ACS6 promoter.Loss-of-function of ACSs suppressed V.dahliae-induced leaf senescence in ORE1-overexpressing plants.Interestingly,PevD1 also interacted with Gossypium hirsutum ORE1(GhORE1),and virus-induced gene silencing of GhORE1 delayed V.dahliae-triggered leaf senescence in cotton,indicative of the existence of a conserved mechanism in plants.Altogether,our study demonstrates that V.dahliae induces leaf senescence by secreting the effector PevD1 to regulate the ORE1-ACS6 cascade,providing new insight into biotic stress-induced senescence in plants.
