Dear Editor The most important property of a virus is its infectivity.To measure infectivity,one can assay viral replication in cells to obtain a titer for a given virus stock.A titer is defined as a given number of i...Dear Editor The most important property of a virus is its infectivity.To measure infectivity,one can assay viral replication in cells to obtain a titer for a given virus stock.A titer is defined as a given number of infectious viral units per unit volume,and an infectious unit is the smallest amount of virus that produces recognizable effects[e.g.,cytopathic effect(CPE),dot blot immunoreactivity].The median tissue culture infectious dose(TCID50)is defined as the dilution of a virus required to infect 50%of a given cell culture.展开更多
Autographa californica multiple nucleopolyhedrovirus(Ac MNPV)orf13(ac13)is a conserved gene in all sequenced alphabaculoviruses.However,its function in the viral life cycle remains unknown.In this study,we found that ...Autographa californica multiple nucleopolyhedrovirus(Ac MNPV)orf13(ac13)is a conserved gene in all sequenced alphabaculoviruses.However,its function in the viral life cycle remains unknown.In this study,we found that ac13 was a late gene and that the encoded protein,bearing a putative nuclear localization signal motif,colocalized with the nuclear lamina.Deletion of ac13 did not affect viral genome replication,nucleocapsid assembly or occlusion body(OB)formation,but reduced virion budding from infected cells by approximately 400-fold compared with the wild-type virus.Deletion of ac13 substantially impaired the egress of nucleocapsids from the nucleus to the cytoplasm,while the OB morphogenesis was unaffected.Taken together,our results indicated that ac13 was required for efficient nuclear egress of nucleocapsids during virion budding,but was dispensable for OB formation.展开更多
基金the support of the National Key Research and Development Program of China(2017YFD0201206)the WIV‘‘One-Three-Five’’strategic programs(Y602111SA1)
文摘Dear Editor The most important property of a virus is its infectivity.To measure infectivity,one can assay viral replication in cells to obtain a titer for a given virus stock.A titer is defined as a given number of infectious viral units per unit volume,and an infectious unit is the smallest amount of virus that produces recognizable effects[e.g.,cytopathic effect(CPE),dot blot immunoreactivity].The median tissue culture infectious dose(TCID50)is defined as the dilution of a virus required to infect 50%of a given cell culture.
基金supported by the National Key Research and Development Program of China(2017YFD0201206)the WIV “One-Three-Five”strategic program(Y602111SA1 to XS)。
文摘Autographa californica multiple nucleopolyhedrovirus(Ac MNPV)orf13(ac13)is a conserved gene in all sequenced alphabaculoviruses.However,its function in the viral life cycle remains unknown.In this study,we found that ac13 was a late gene and that the encoded protein,bearing a putative nuclear localization signal motif,colocalized with the nuclear lamina.Deletion of ac13 did not affect viral genome replication,nucleocapsid assembly or occlusion body(OB)formation,but reduced virion budding from infected cells by approximately 400-fold compared with the wild-type virus.Deletion of ac13 substantially impaired the egress of nucleocapsids from the nucleus to the cytoplasm,while the OB morphogenesis was unaffected.Taken together,our results indicated that ac13 was required for efficient nuclear egress of nucleocapsids during virion budding,but was dispensable for OB formation.
