[Objectives] To observe the clinical efficacy of Jiawei Buzhong Yiqi Decoction in the treatment of polycystic ovarian syndrome(PCOS).[Methods]Obese patients with PCOS were recruited for the study in September 2014-Dec...[Objectives] To observe the clinical efficacy of Jiawei Buzhong Yiqi Decoction in the treatment of polycystic ovarian syndrome(PCOS).[Methods]Obese patients with PCOS were recruited for the study in September 2014-December 2016 from Chinese medicine gynecological clinic in the affiliated hospital of Hubei University of Medicine.All these patients were given one dose of Jiawei Buzhong Yiqi Decoction one time a day for three months.Body mass index(BMI) was measured before and after treatment.Sex hormone levels and ovulation were measured,and the improvement of clinical symptoms was compared before and after treatment.[Results] After treatment,the levels of BMI and serum luteinizing hormone(LH),testosterone(T) were significantly decreased,and the level of serum estradiol(E2) was significantly higher than that before treatment.Ovarian enlargement and polycystic changes,menstrual cycle,menstrual flow and menstrual period were improved in varying degrees.[Conclusions] Jiawei Buzhong Yiqi Decoction has a good clinical effect in the treatment of obese PCOS.展开更多
Chronic cerebral hypoperfusion leads to white matter injury(WMI),which subsequently causes neurodegeneration and even cognitive impairment.However,due to the lack of treatment specifically for WMI,novel recognized and...Chronic cerebral hypoperfusion leads to white matter injury(WMI),which subsequently causes neurodegeneration and even cognitive impairment.However,due to the lack of treatment specifically for WMI,novel recognized and effective therapeutic strategies are urgently needed.In this study,we found that honokiol and magnolol,two compounds derived from Magnolia officinalis,significantly facilitated the differentiation of primary oligodendrocyte precursor cells(OPCs)into mature oligodendrocytes,with a more prominent effect of the former compound.Moreover,our results demonstrated that honokiol treatment improved myelin injury,induced mature oligodendrocyte protein expression,attenuated cognitive decline,promoted oligodendrocyte regeneration,and inhibited astrocytic activation in the bilateral carotid artery stenosis model.Mechanistically,honokiol increased the phosphorylation of serine/threonine kinase(Akt)and mammalian target of rapamycin(mTOR)by activating cannabinoid receptor 1 during OPC differentiation.Collectively,our study indicates that honokiol might serve as a potential treatment for WMI in chronic cerebral ischemia.展开更多
Extracellularβ-amyloid(Aβ)plaques and neurofibrillary tangles(NFTs)are the pathological hallmarks of Alzheimer’s disease(AD).Studies have shown that aggregates of extracellular Aβcan induce neuroinflammation media...Extracellularβ-amyloid(Aβ)plaques and neurofibrillary tangles(NFTs)are the pathological hallmarks of Alzheimer’s disease(AD).Studies have shown that aggregates of extracellular Aβcan induce neuroinflammation mediated neurotoxic signaling through microglial activation and release of pro-inflammatory factors.Thus,modulation of Aβmight be a potential therapeutic strategy for modifying disease progression.Recently,a large number of reports have confirmed the beneficial effects of mesenchymal stem cells(MSCs)on AD.It is believed to reduce neuroinflammation,reduce Aβamyloid deposits and NFTs,increase acetylcholine levels,promote neurogenesis,reduce neuronal damage,and improve working memory and cognition.In this review,we focus on the role of MSCs in clearing Aβdeposition.MSCs have the potential to modulate Aβ-related microenvironments via enhancement of autophagy,proteolysis of Aβaggregates,phagocytic clearance of Aβby microglial M2 polarization,decrease oxidative stress(OS),and correction of abnormal sphingolipid(SL)metabolism.With advantages in clinical applications,these data suggest that the use of MSCs as a multi-target modulator of Aβwould be an effective therapeutic approach in AD.展开更多
[Objectives]The study aims to discuss the effects of Jiawei Buzhong Yiqi Decoction on the endometrial receptivity of rats with polycystic ovarian syndrome(PCOS).[Methods]Adult female SD rats were selected and randomly...[Objectives]The study aims to discuss the effects of Jiawei Buzhong Yiqi Decoction on the endometrial receptivity of rats with polycystic ovarian syndrome(PCOS).[Methods]Adult female SD rats were selected and randomly divided into the model group and control group.In the model group,the PCOS model was established by using the method of Poretsky L.Rats in the control group were given physiological saline intervention at the same time.The successfully modeled rats were randomly divided into the PCOS group and traditional Chinese medicine group.Among them,rats in the Chinese medicine group were given Jiawei Buzhong Yiqi Decoction by gavage;rats in the model group and the control group were given normal saline for 14 d.All rats were killed by anesthesia after the last administration,and serum and uterine specimens were collected.Serum sex hormones(including T,E_(2),and P),glucose and insulin levels were detected;the equivalent diameter and area of endometrial gland and gland cavity,and the endometrial thickness of rats were measured;the expression of endometrial leukemia inhibitory factor(LIF),integrinαvβ3 protein and mRNA was detected by using Elisa and RT-PCR methods.[Results]The serum T,glucose,and insulin levels of rats in the PCOS group were significantly higher than those of the control group and the traditional Chinese medicine group,and there was no significant difference in the above indicators between the control group and the traditional Chinese medicine group;there was no significant difference in the E_(2) and P levels between the three groups.The equivalent diameter and area of endometrial gland and gland cavity,and the endometrial thickness of rats in the PCOS group were lower than those of the control group and the traditional Chinese medicine group;there was no significant difference in the above indicators between the traditional Chinese medicine group and the control group.The expression of LIF,integrinαvβ3 protein and mRNA in the endometrium of rats in various groups was basically the same.The expression of LIF and integrinαvβ3 in the endometrium of rats in the PCOS group was significantly lower than that of the control group and the traditional Chinese medicine group;there was no significant difference in the above indicators between the control group and the traditional Chinese medicine group.[Conclusions]The endometrial receptivity of PCOS rats was decreased;Jiawei Buzhong Yiqi Decoction can improve the endometrial receptivity of PCOS rats by increasing the expression of LIF and integrinαvβ3 in the endometrium.展开更多
As a service format to meet spiritual needs, fashion culture industry is often synchronized in its creation, production, dissemination, display and consumption. In order to explore how artificial intelligence provides...As a service format to meet spiritual needs, fashion culture industry is often synchronized in its creation, production, dissemination, display and consumption. In order to explore how artificial intelligence provides technical means, platforms, channels and space for intelligent formats in the field of communication, and how to provide intelligent services for cultural creation, communication, display and consumption. This paper discusses the application and innovation of artificial intelligence in all aspects of communication field, analyzes the problems and puts forward corresponding countermeasures and suggestions.展开更多
A novel processing route involving strip casting, pre-annealing treatment, cold rolling and recrystallization annealing was applied to a Fe-2.6%Si steel to improve the magnetic properties. The impact of as-cast strip ...A novel processing route involving strip casting, pre-annealing treatment, cold rolling and recrystallization annealing was applied to a Fe-2.6%Si steel to improve the magnetic properties. The impact of as-cast strip pre-annealing on the microstructure, texture, precipitation and magnetic properties were investigated by electron probe micro-analysis, transmission electron microscopy, and X-ray diffraction analysis,etc. It was found that the precipitation of second-phase particles during strip casting was restrained by rapid solidification. The absence of pre-annealing led to the occurrence of a large amount of 20-50 nm Mn S precipitates in the final annealed sheets, which is responsible for fine grains and high core loss(4.01 W/kg) due to grain boundary pinning effect. Although the microstructure and texture of 900-1000?C pre-annealed samples were similar to those of as-cast strip, significant grain coarsening together with the strengthening of-fiber texture was observed in the 1100?C pre-annealed strips. In comparison with the case of as-cast strip, a higher amount of large-sized precipitates consisting of manganese sulfide and/or aluminum nitride occurred in matrix after pre-annealing. Correspondingly, in the final annealed sheets, the number density of precipitates with sizes smaller than 100 nm was substantially reduced, and100-200 nm and 200-500 nm sized particles became more dominant in samples subjected to 30-min and 120-min pre-annealing treatments respectively. In addition, the average grain size of final annealed sheets increased with the pre-annealing temperature and time because of the weakened pining effect of coarsen precipitates. Ultimately, the magnetic induction of samples subjected to pre-annealing was slightly increased and ranged from 1.73 T to 1.75 T owing to the enhancement of {100} recrystallization texture, and simultaneously the core loss significantly decreased until a minimum of 3.26 W/kg was reached. Nevertheless, large number of 200-500 nm particles presented during pre-annealing for 120 min could weaken the improvement in core loss which is likely associated with the pinning effect on magnetic domain wall.展开更多
In 2009,a novel swine-origin H1N1 influenza virus emerged in Mexico and quickly spread to other countries,including China.This 2009 pandemic H1N1 can cause human respiratory disease,but its pathogenesis remains poorly...In 2009,a novel swine-origin H1N1 influenza virus emerged in Mexico and quickly spread to other countries,including China.This 2009 pandemic H1N1 can cause human respiratory disease,but its pathogenesis remains poorly understood.Here,we studied the infection and pathogenesis of a new 2009 pandemic strain,A/Wenshan/01/2009 H1N1,in China in human airway epithelial cell lines compared with contemporary seasonal H1N1 influenza virus.Our results showed that viral infection by the A/Wenshan H1N1 induced significant apoptotic cell death in both the human nasopharyngeal carcinoma cell line CNE-2Z and the human lung adenocarcinoma cell line A549.The A/Wenshan H1N1 virus enters both of these cell types more efficiently than the seasonal influenza virus.Viral entry in both cell lines was shown to be mediated by clathrin-and dynamin-dependent endocytosis.Therefore,we discovered that the 2009 pandemic H1N1 strain,A/Wenshan/01/2009,can induce apoptotic cell death in epithelial cells of the human respiratory tract,suggesting a molecular pathogenesis for the 2009 pandemic H1N1.展开更多
Aluminum nitride (AIN) precipitates and microstructure of 4 wt.% (Si+AI) non-oriented electrical steel were investigated. The 2.0 mm thick cast strips with three different silicon/aluminum (Si/AI) ratios were p...Aluminum nitride (AIN) precipitates and microstructure of 4 wt.% (Si+AI) non-oriented electrical steel were investigated. The 2.0 mm thick cast strips with three different silicon/aluminum (Si/AI) ratios were produced by twin-roll casting process, then the strips were reheated, warm rolled, cold rolled and annealed. The microstructure and AIN precipitates were characterized using optical microscopy, scanning electron microscopy and transmission electron microscopy. The results showed that with the increase of Si/AI ratio, on the one hand, the casting microstructure changed from columnar grains to equiaxed grains, and the uniformity of annealed microstructure was improved; On the other hand, the number of AIN precipitates in cast strips reduced meanwhile the distribution became dispersed. By the reheat treatment, the size and distribution of the AIN precipitates can be changed. Moreover, the grain size of the annealed strips is in the range of 20-50 #m, at the same time, many AIN precipitates were located at grain boundaries. Therefore, controlling the Si/AI ratio is a simple method to obtain desired microstructure. Then AIN precipitates in non-oriented electrical steel prepared by twin-roll casting process hinder markedly the recrystallized grains growth, A compatible reheat treatment can be an approach worth exploring to control the behavior of AIN precipitates.展开更多
基金Supported by Scientific Research Guidance Project of Hubei Provincial Department of Education(B2018437)Scientific Research Guiding Project of Shiyan City,Hubei Province(18Y08)
文摘[Objectives] To observe the clinical efficacy of Jiawei Buzhong Yiqi Decoction in the treatment of polycystic ovarian syndrome(PCOS).[Methods]Obese patients with PCOS were recruited for the study in September 2014-December 2016 from Chinese medicine gynecological clinic in the affiliated hospital of Hubei University of Medicine.All these patients were given one dose of Jiawei Buzhong Yiqi Decoction one time a day for three months.Body mass index(BMI) was measured before and after treatment.Sex hormone levels and ovulation were measured,and the improvement of clinical symptoms was compared before and after treatment.[Results] After treatment,the levels of BMI and serum luteinizing hormone(LH),testosterone(T) were significantly decreased,and the level of serum estradiol(E2) was significantly higher than that before treatment.Ovarian enlargement and polycystic changes,menstrual cycle,menstrual flow and menstrual period were improved in varying degrees.[Conclusions] Jiawei Buzhong Yiqi Decoction has a good clinical effect in the treatment of obese PCOS.
基金This work was supported by the National Natural Science Foundation of China(81920108017 and 82130036)the Key Research and Development Program of Jiangsu Province of China(BE2020620)the Jiangsu Province Key Medical Discipline(ZDXKA2016020).
文摘Chronic cerebral hypoperfusion leads to white matter injury(WMI),which subsequently causes neurodegeneration and even cognitive impairment.However,due to the lack of treatment specifically for WMI,novel recognized and effective therapeutic strategies are urgently needed.In this study,we found that honokiol and magnolol,two compounds derived from Magnolia officinalis,significantly facilitated the differentiation of primary oligodendrocyte precursor cells(OPCs)into mature oligodendrocytes,with a more prominent effect of the former compound.Moreover,our results demonstrated that honokiol treatment improved myelin injury,induced mature oligodendrocyte protein expression,attenuated cognitive decline,promoted oligodendrocyte regeneration,and inhibited astrocytic activation in the bilateral carotid artery stenosis model.Mechanistically,honokiol increased the phosphorylation of serine/threonine kinase(Akt)and mammalian target of rapamycin(mTOR)by activating cannabinoid receptor 1 during OPC differentiation.Collectively,our study indicates that honokiol might serve as a potential treatment for WMI in chronic cerebral ischemia.
基金supported by a grant from the National Science Foundation of China(No.82001326)Key R&D project of Shanxi Province(201803D31068)+1 种基金Applied Basic Research Project of Shanxi Province(201901D211319,201901D111384)Science and Technology Innovation Project of Colleges and Universities of Shanxi Province(2019L0445,2019L0418).
文摘Extracellularβ-amyloid(Aβ)plaques and neurofibrillary tangles(NFTs)are the pathological hallmarks of Alzheimer’s disease(AD).Studies have shown that aggregates of extracellular Aβcan induce neuroinflammation mediated neurotoxic signaling through microglial activation and release of pro-inflammatory factors.Thus,modulation of Aβmight be a potential therapeutic strategy for modifying disease progression.Recently,a large number of reports have confirmed the beneficial effects of mesenchymal stem cells(MSCs)on AD.It is believed to reduce neuroinflammation,reduce Aβamyloid deposits and NFTs,increase acetylcholine levels,promote neurogenesis,reduce neuronal damage,and improve working memory and cognition.In this review,we focus on the role of MSCs in clearing Aβdeposition.MSCs have the potential to modulate Aβ-related microenvironments via enhancement of autophagy,proteolysis of Aβaggregates,phagocytic clearance of Aβby microglial M2 polarization,decrease oxidative stress(OS),and correction of abnormal sphingolipid(SL)metabolism.With advantages in clinical applications,these data suggest that the use of MSCs as a multi-target modulator of Aβwould be an effective therapeutic approach in AD.
基金Chinese medicine youth project of Hubei Provincial Health Commission(ZY2021Q017).
文摘[Objectives]The study aims to discuss the effects of Jiawei Buzhong Yiqi Decoction on the endometrial receptivity of rats with polycystic ovarian syndrome(PCOS).[Methods]Adult female SD rats were selected and randomly divided into the model group and control group.In the model group,the PCOS model was established by using the method of Poretsky L.Rats in the control group were given physiological saline intervention at the same time.The successfully modeled rats were randomly divided into the PCOS group and traditional Chinese medicine group.Among them,rats in the Chinese medicine group were given Jiawei Buzhong Yiqi Decoction by gavage;rats in the model group and the control group were given normal saline for 14 d.All rats were killed by anesthesia after the last administration,and serum and uterine specimens were collected.Serum sex hormones(including T,E_(2),and P),glucose and insulin levels were detected;the equivalent diameter and area of endometrial gland and gland cavity,and the endometrial thickness of rats were measured;the expression of endometrial leukemia inhibitory factor(LIF),integrinαvβ3 protein and mRNA was detected by using Elisa and RT-PCR methods.[Results]The serum T,glucose,and insulin levels of rats in the PCOS group were significantly higher than those of the control group and the traditional Chinese medicine group,and there was no significant difference in the above indicators between the control group and the traditional Chinese medicine group;there was no significant difference in the E_(2) and P levels between the three groups.The equivalent diameter and area of endometrial gland and gland cavity,and the endometrial thickness of rats in the PCOS group were lower than those of the control group and the traditional Chinese medicine group;there was no significant difference in the above indicators between the traditional Chinese medicine group and the control group.The expression of LIF,integrinαvβ3 protein and mRNA in the endometrium of rats in various groups was basically the same.The expression of LIF and integrinαvβ3 in the endometrium of rats in the PCOS group was significantly lower than that of the control group and the traditional Chinese medicine group;there was no significant difference in the above indicators between the control group and the traditional Chinese medicine group.[Conclusions]The endometrial receptivity of PCOS rats was decreased;Jiawei Buzhong Yiqi Decoction can improve the endometrial receptivity of PCOS rats by increasing the expression of LIF and integrinαvβ3 in the endometrium.
文摘As a service format to meet spiritual needs, fashion culture industry is often synchronized in its creation, production, dissemination, display and consumption. In order to explore how artificial intelligence provides technical means, platforms, channels and space for intelligent formats in the field of communication, and how to provide intelligent services for cultural creation, communication, display and consumption. This paper discusses the application and innovation of artificial intelligence in all aspects of communication field, analyzes the problems and puts forward corresponding countermeasures and suggestions.
基金financially supported by the National Natural Science Foundation of China(Nos.51674080,51404155 and U1260204)the Program for New Century Excellent Talents in University(No.NCET-13-0111)+1 种基金the Program for Liaoning Excellent Talents in University(No.LR2014007)support from the University of Texas at El Paso
文摘A novel processing route involving strip casting, pre-annealing treatment, cold rolling and recrystallization annealing was applied to a Fe-2.6%Si steel to improve the magnetic properties. The impact of as-cast strip pre-annealing on the microstructure, texture, precipitation and magnetic properties were investigated by electron probe micro-analysis, transmission electron microscopy, and X-ray diffraction analysis,etc. It was found that the precipitation of second-phase particles during strip casting was restrained by rapid solidification. The absence of pre-annealing led to the occurrence of a large amount of 20-50 nm Mn S precipitates in the final annealed sheets, which is responsible for fine grains and high core loss(4.01 W/kg) due to grain boundary pinning effect. Although the microstructure and texture of 900-1000?C pre-annealed samples were similar to those of as-cast strip, significant grain coarsening together with the strengthening of-fiber texture was observed in the 1100?C pre-annealed strips. In comparison with the case of as-cast strip, a higher amount of large-sized precipitates consisting of manganese sulfide and/or aluminum nitride occurred in matrix after pre-annealing. Correspondingly, in the final annealed sheets, the number density of precipitates with sizes smaller than 100 nm was substantially reduced, and100-200 nm and 200-500 nm sized particles became more dominant in samples subjected to 30-min and 120-min pre-annealing treatments respectively. In addition, the average grain size of final annealed sheets increased with the pre-annealing temperature and time because of the weakened pining effect of coarsen precipitates. Ultimately, the magnetic induction of samples subjected to pre-annealing was slightly increased and ranged from 1.73 T to 1.75 T owing to the enhancement of {100} recrystallization texture, and simultaneously the core loss significantly decreased until a minimum of 3.26 W/kg was reached. Nevertheless, large number of 200-500 nm particles presented during pre-annealing for 120 min could weaken the improvement in core loss which is likely associated with the pinning effect on magnetic domain wall.
基金supported by the Ministry of Science and Technology(2009CB522105)the Ministry of Health(2009ZX10004-308)of Chinasupport of the Science and Technology Commission of Shanghai Municipality(07pj14096).
文摘In 2009,a novel swine-origin H1N1 influenza virus emerged in Mexico and quickly spread to other countries,including China.This 2009 pandemic H1N1 can cause human respiratory disease,but its pathogenesis remains poorly understood.Here,we studied the infection and pathogenesis of a new 2009 pandemic strain,A/Wenshan/01/2009 H1N1,in China in human airway epithelial cell lines compared with contemporary seasonal H1N1 influenza virus.Our results showed that viral infection by the A/Wenshan H1N1 induced significant apoptotic cell death in both the human nasopharyngeal carcinoma cell line CNE-2Z and the human lung adenocarcinoma cell line A549.The A/Wenshan H1N1 virus enters both of these cell types more efficiently than the seasonal influenza virus.Viral entry in both cell lines was shown to be mediated by clathrin-and dynamin-dependent endocytosis.Therefore,we discovered that the 2009 pandemic H1N1 strain,A/Wenshan/01/2009,can induce apoptotic cell death in epithelial cells of the human respiratory tract,suggesting a molecular pathogenesis for the 2009 pandemic H1N1.
基金financially supported by the National Natural Science Foundation of China(Nos.50734001 and 51004035)
文摘Aluminum nitride (AIN) precipitates and microstructure of 4 wt.% (Si+AI) non-oriented electrical steel were investigated. The 2.0 mm thick cast strips with three different silicon/aluminum (Si/AI) ratios were produced by twin-roll casting process, then the strips were reheated, warm rolled, cold rolled and annealed. The microstructure and AIN precipitates were characterized using optical microscopy, scanning electron microscopy and transmission electron microscopy. The results showed that with the increase of Si/AI ratio, on the one hand, the casting microstructure changed from columnar grains to equiaxed grains, and the uniformity of annealed microstructure was improved; On the other hand, the number of AIN precipitates in cast strips reduced meanwhile the distribution became dispersed. By the reheat treatment, the size and distribution of the AIN precipitates can be changed. Moreover, the grain size of the annealed strips is in the range of 20-50 #m, at the same time, many AIN precipitates were located at grain boundaries. Therefore, controlling the Si/AI ratio is a simple method to obtain desired microstructure. Then AIN precipitates in non-oriented electrical steel prepared by twin-roll casting process hinder markedly the recrystallized grains growth, A compatible reheat treatment can be an approach worth exploring to control the behavior of AIN precipitates.