In animal cells, action of acetylcholine depends on its binding with its two specific receptors on the plasma membrane: the nicotinic and muscarinic respectively. The present investigation has shown that agonists of m...In animal cells, action of acetylcholine depends on its binding with its two specific receptors on the plasma membrane: the nicotinic and muscarinic respectively. The present investigation has shown that agonists of muscarinic receptor (muscarine) could induce stomatal opening, while the antagonists (atropine) could block stomatal opening induced by acetylcholine. Their effects can only be realized in medium containing Ca2+, but not in medium containing K+. The results tend to reveal that the muscarinic receptor is involved in acetylcholine-induced stomatal movement.展开更多
Acetylcholine (ACh), as an important neuro-transmitter in animals, also plays a significant role in various kinds of physiological functions in plants. But relatively little is known about its receptors in plants. A g...Acetylcholine (ACh), as an important neuro-transmitter in animals, also plays a significant role in various kinds of physiological functions in plants. But relatively little is known about its receptors in plants. A green fluorescence BODIPY FL-labeled ABT, which is a high affinity ligand of muscarinic acetylcholine receptor (mAChR), was used to localize mAChR in plant guard cells. In Vicia faba L. and Pisum sativum L., mAChR was found both on the plasma membrane of guard cells. mAChR may also be distributed on guard cell chloroplast membrane of Vicia faba L. The evidence that mAChR localizes in the guard cells provides a new possible signal transduction pathway in ACh mediated stomata movement.展开更多
The stoma of the plant leaf and the factors that elicit movement of its guard cells are taken as a grey system. Effects of temporary light exposure on local electrical potential of the leaf surface (LEP), on CO 2 and ...The stoma of the plant leaf and the factors that elicit movement of its guard cells are taken as a grey system. Effects of temporary light exposure on local electrical potential of the leaf surface (LEP), on CO 2 and on relative humidity (RH) changes within the leaf chamber were recorded with a multi-channel electronic recorder. The results are analyzed by employing the grey relation function ( ξ ) and grey relation grade ( R ) based on the Grey Theory. The analysis shows that LEP could represent the dominant factor in executing the stomatal movement under the light exposure.展开更多
文摘In animal cells, action of acetylcholine depends on its binding with its two specific receptors on the plasma membrane: the nicotinic and muscarinic respectively. The present investigation has shown that agonists of muscarinic receptor (muscarine) could induce stomatal opening, while the antagonists (atropine) could block stomatal opening induced by acetylcholine. Their effects can only be realized in medium containing Ca2+, but not in medium containing K+. The results tend to reveal that the muscarinic receptor is involved in acetylcholine-induced stomatal movement.
基金the National NaturalScience Foundation of China (Grant No. 39870076) and the State Key Basic Research Development Program of China (Grant No. G19990117).
文摘Acetylcholine (ACh), as an important neuro-transmitter in animals, also plays a significant role in various kinds of physiological functions in plants. But relatively little is known about its receptors in plants. A green fluorescence BODIPY FL-labeled ABT, which is a high affinity ligand of muscarinic acetylcholine receptor (mAChR), was used to localize mAChR in plant guard cells. In Vicia faba L. and Pisum sativum L., mAChR was found both on the plasma membrane of guard cells. mAChR may also be distributed on guard cell chloroplast membrane of Vicia faba L. The evidence that mAChR localizes in the guard cells provides a new possible signal transduction pathway in ACh mediated stomata movement.
文摘The stoma of the plant leaf and the factors that elicit movement of its guard cells are taken as a grey system. Effects of temporary light exposure on local electrical potential of the leaf surface (LEP), on CO 2 and on relative humidity (RH) changes within the leaf chamber were recorded with a multi-channel electronic recorder. The results are analyzed by employing the grey relation function ( ξ ) and grey relation grade ( R ) based on the Grey Theory. The analysis shows that LEP could represent the dominant factor in executing the stomatal movement under the light exposure.
