Aptasensing biosynthesized phosphatidylserine with a AuNPs nanozyme-based colorimetric aptasensor

Sensitive monitoring of the target products during the biosynthesis process is crucial,and facile analytical approaches are urgently needed.Herein,phosphatidylserine(PS)was chosen as the model target,a colorimetric aptasensor was developed for the rapid quantitation in biosynthesis samples.A chimeric aptamer was constructed with two homogeneous original PS aptamers.Specific recognition between the chimeric aptamer and PS results in the desorption of aptamer from the surface of the AuNPs nanozyme,and the peroxidase-like enzymatic activity of the AuNPs nanozyme was weakened in a relationship with the different concentrations.The developed aptasensor performed well when applied for analyzing PS in biosynthesis samples.The aptasensor offers good sensitivity and selectivity,under optimal conditions,achieving monitoring and quantitation of PS in the range of 2.5-80.0μmol/L,with a limit of detection at 536.2 nmol/L.Moreover,the aptasensor provides good accuracy,with comparison rates of 98.17%-106.40%,when compared with the HPLC-ELSD.This study provides a good reference for monitoring other biosynthesized products and promoting the development of aptamers and aptasensors in real-world applications.

The Review of the Welding Robot’s Precision

Welding is an significant link in industrial production,and it is known as the"industrial tailor".However,welding fume,arc light,and metal spatter cause a harsh welding working environment,and the quality of welding has a decisive influence on product quality.Low production capacity,difficult recruitment,and low profits have become drawbacks for the development of the welding field.Combining traditional welding with robots can solve these drawbacks and increase the precision of welding.Welding robots are industrial robots engaged in welding and are mostly used in large-scale manufacturing fields such as automobile manufacturing.Welding robots are divided into spot welding robots,arc welding robots and laser welding robots.In addition,body accuracy and control accuracy are the two main factors that affect the robot welding accuracy.In this case,the sensing technology of welding robot is also essential.

Treatmentsforresectableesophageal cancer:from traditional systemic therapy to immunotherapy

Esophageal cancer(EC)has a high incidence and poor prognosis.The two major histological types,squamous cell carcinoma and adenocarcinoma,differ in their epidemiology and treatment options.Patients with locally advanced EC benefit from multimodal therapy concepts including neoadjuvant chemotherapy,neoadjuvant chemoradiotherapy,and perioperative chemotherapy.Currently,immunotherapy for the solid tumor is a hot spot.Treatment with adjuvant immune checkpoint inhibitors(ICIs)is the first immunotherapy for resectable EC listed in the latest National Comprehensive Cancer Network Guidelines for the Esophageal and Esophagogastric Junction Cancers.Recent clinical trials have established ICIs for three treatment models of resectable EC.Their short-term results demonstrated ideal efficacy and tolerable toxicity,though some concerns remain.This review summarizes the novel data on the ICIs for resectable EC and lists the registered related clinical trials.Hopefully,this review can provide a reference for ongoing research on the treatment options for resectable EC.

Dose escalation guided by^(18)F-FDG PET/CT for esophageal cancer

Objective:To assess the feasibility of dose escalation guided by ^(18)F-deoxyglucose positron emission tomography/computer tomography(^(18)F-FDG PET/CT)for esophageal cancer(EC).Methods and materials:Ten random patients treated with definitive chemoradiotherapy and pre-therapeutic ^(18)FFDG PET/CT were included in this study.Retrospectively,a threshold of 50%of SUVmax was used to define the high FDG uptake region of the GTV(GTVPET).Three intensity-modulated radiation therapy(IMRT)plans were generated,delivering three dose levels to three different planning target volumes(PTVs).50.4 Gy delivered to PTV50.4 was defined on computed tomography(CT)as Plan50.4,63 Gy was delivered to PTV63 was defined as GTV plus a uniform margin of 0.5 cm as Plan63,and 70 Gy delivered to PTV70 was defined as GTVPET plus a 0.5 cm margin as Plan70.A dosimetric comparison was performed based on normal tissue complication probability(NTCP)for the lung and heart.Results:Clinically acceptable dose escalation failed for 2 of 10 patients in Plan63 due to heart dose constraints.One patient failed heart dose constraint for Plan70.Two patients failed spinal cord constraint for Plan63.Three patients failed lung dose constraints for both Plan63 and Plan70,two of which were even not suitable for Plan50.4 for the same reason.NTCP modeling for lung showed increased risk for Plan63 and Plan70 compared to Plan50.4.The difference between Plan63 and Plan70 was insignificant.NTCP modeling for heart showed an increased risk from 6.38%of Plan50.4 to 8.88%of Plan70(P=0.009)or 9.79%of Plan63(P=0.007).The risk of heart mortality was significantly higher for Plan63 than Plan70(P=0.047).Conclusions:Selective boosting of sub-volumes based on ^(18)F-FDG PET/CT is feasible way with a modest increase in the risk of cardiac and lung toxicities.