Light field microscopy(LFM),featured for high threedimensional imaging speed and low pho-totoxicity,has emeged as a technique of choice for instantaneous volumetric inaging.In contrast with other scanning-based three ...Light field microscopy(LFM),featured for high threedimensional imaging speed and low pho-totoxicity,has emeged as a technique of choice for instantaneous volumetric inaging.In contrast with other scanning-based three dimensional(3D)imaging approaches,LFM enables to encode 3D spatial information in a snapshot manner,permitting high-speed 3D imaging that is only limited by the frame rate of the camera.In this review,we first introduce the fundamental theory of LFM and current corresponding advanced approaches.Then,we summarize various applica-tions of LFM in biological imaging.展开更多
基金supports offered by the National Natural Science Foundation of China (T2225014,21874052,61860206009)the National Key Research and Development Program of China (2017YFA0700501).
文摘Light field microscopy(LFM),featured for high threedimensional imaging speed and low pho-totoxicity,has emeged as a technique of choice for instantaneous volumetric inaging.In contrast with other scanning-based three dimensional(3D)imaging approaches,LFM enables to encode 3D spatial information in a snapshot manner,permitting high-speed 3D imaging that is only limited by the frame rate of the camera.In this review,we first introduce the fundamental theory of LFM and current corresponding advanced approaches.Then,we summarize various applica-tions of LFM in biological imaging.
