Developing methylotrophic cell factories that can efficiently catalyze organic one-carbon(C1)feedstocks derived from electrocatalytic reduction of carbon dioxide into bio-based chemicals and biofuels is of strategic s...Developing methylotrophic cell factories that can efficiently catalyze organic one-carbon(C1)feedstocks derived from electrocatalytic reduction of carbon dioxide into bio-based chemicals and biofuels is of strategic significance for building a carbon-neutral,sustainable economic and industrial system.With the rapid advancement of RNA sequencing technology and mass spectrometer analysis,researchers have used these quantitative microbiology methods extensively,especially isotope-based metabolic flux analysis,to study the metabolic processes initiating from C1 feedstocks in natural C1-utilizing bacteria and synthetic C1 bacteria.This paper reviews the use of advanced quantitative analysis in recent years to understand the metabolic network and basic principles in the metabolism of natural C1-utilizing bacteria grown on methane,methanol,or formate.The acquired knowledge serves as a guide to rewire the central methylotrophic metabolism of natural C1-utilizing bacteria to improve the carbon conversion efficiency,and to engineer non-C1-utilizing bacteria into synthetic strains that can use C1 feedstocks as the sole carbon and energy source.These progresses ultimately enhance the design and construction of highly efficient C1-based cell factories to synthesize diverse high value-added products.The integration of quantitative biology and synthetic biology will advance the iterative cycle of understand–design–build–testing–learning to enhance C1-based biomanufacturing in the future.展开更多
In recent years, nanozymes have received more and more attention, but the low activity limits the development of nanozymes. Therefore, the design and development of efficient nanozymes is still a major challenge for r...In recent years, nanozymes have received more and more attention, but the low activity limits the development of nanozymes. Therefore, the design and development of efficient nanozymes is still a major challenge for researchers. Herein, the Fe,N co-doped ultrathin hollow carbon framework(Fe,N-UHCF) exhibit ultra-high peroxidase-like activity. The specific activity of Fe,N-UHCF nanozyme is as high as 36.6 U/mg,which is much higher than almost all of other reported nanozymes. In practical applications, the Fe,N-UHCF show good antibacterial effects.展开更多
Due to the existence of dual-role factors,it is difficult to evaluate the production efficiency of two-stage systems.Unlike single-stage systems,two-stage systems involve intermediate products that serve as both input...Due to the existence of dual-role factors,it is difficult to evaluate the production efficiency of two-stage systems.Unlike single-stage systems,two-stage systems involve intermediate products that serve as both inputs and outputs.Hence,to overcome existing obstacles,we propose a novel approach called the two-stage enhanced Russell model with dual-role factors(T-ERM-D)to assess the overall efficiency of two-stage production systems.Furthermore,divisional models are developed to evaluate the efficiency of each individual stage.The 0-1 programming is applied to deal with dual-role factors.To handle the non-linearity of these models,the Charnes-Cooper transformation is employed to convert them into linear ones.Using the proposed models,we evaluate efficiency scores of 10 supply chains involving suppliers and producers.By comparing the results obtained from new models with those obtained from models that do not consider dual-role factors,we validate the advantages of the proposed approach.展开更多
基金National Key R&D Program of China,Grant Award Numbers:2018YFA0901500,2021YFC2103500National Natural Science Foundation of China,Grant/Award Numbers:22078169,32000003,31900004。
文摘Developing methylotrophic cell factories that can efficiently catalyze organic one-carbon(C1)feedstocks derived from electrocatalytic reduction of carbon dioxide into bio-based chemicals and biofuels is of strategic significance for building a carbon-neutral,sustainable economic and industrial system.With the rapid advancement of RNA sequencing technology and mass spectrometer analysis,researchers have used these quantitative microbiology methods extensively,especially isotope-based metabolic flux analysis,to study the metabolic processes initiating from C1 feedstocks in natural C1-utilizing bacteria and synthetic C1 bacteria.This paper reviews the use of advanced quantitative analysis in recent years to understand the metabolic network and basic principles in the metabolism of natural C1-utilizing bacteria grown on methane,methanol,or formate.The acquired knowledge serves as a guide to rewire the central methylotrophic metabolism of natural C1-utilizing bacteria to improve the carbon conversion efficiency,and to engineer non-C1-utilizing bacteria into synthetic strains that can use C1 feedstocks as the sole carbon and energy source.These progresses ultimately enhance the design and construction of highly efficient C1-based cell factories to synthesize diverse high value-added products.The integration of quantitative biology and synthetic biology will advance the iterative cycle of understand–design–build–testing–learning to enhance C1-based biomanufacturing in the future.
基金supported by the National Natural Science Foundation of China(NSFC,Nos.21671149,21571140,21531005 and 21703156)the 973 Program(No.2014CB845601)+3 种基金the Program for Innovative Research Team in University of Tianjin(No.TD13–5074)the Natural Science Foundation of Tianjin(No.18JCQNJC76000)the Science&Technology Development Fund of Tianjin Education Commission for Higher Education(No.2021KJ190)the Jiangsu Provincial Double-Innovation Doctor Program(No.02300053)。
文摘In recent years, nanozymes have received more and more attention, but the low activity limits the development of nanozymes. Therefore, the design and development of efficient nanozymes is still a major challenge for researchers. Herein, the Fe,N co-doped ultrathin hollow carbon framework(Fe,N-UHCF) exhibit ultra-high peroxidase-like activity. The specific activity of Fe,N-UHCF nanozyme is as high as 36.6 U/mg,which is much higher than almost all of other reported nanozymes. In practical applications, the Fe,N-UHCF show good antibacterial effects.
基金Supported by the National Natural Science Foundation of China(72262024)the Program for Young Talents of Science and Technology in Universities of Inner Mongolia Autonomous Region(NJYT22095)。
文摘Due to the existence of dual-role factors,it is difficult to evaluate the production efficiency of two-stage systems.Unlike single-stage systems,two-stage systems involve intermediate products that serve as both inputs and outputs.Hence,to overcome existing obstacles,we propose a novel approach called the two-stage enhanced Russell model with dual-role factors(T-ERM-D)to assess the overall efficiency of two-stage production systems.Furthermore,divisional models are developed to evaluate the efficiency of each individual stage.The 0-1 programming is applied to deal with dual-role factors.To handle the non-linearity of these models,the Charnes-Cooper transformation is employed to convert them into linear ones.Using the proposed models,we evaluate efficiency scores of 10 supply chains involving suppliers and producers.By comparing the results obtained from new models with those obtained from models that do not consider dual-role factors,we validate the advantages of the proposed approach.
