Heading date(or flowering time),an important agronomic trait in crop species,is closely associated with regional adaptation and yield.Members of the Pseudo-Response Regulator(PRR)family play key roles in regulating fl...Heading date(or flowering time),an important agronomic trait in crop species,is closely associated with regional adaptation and yield.Members of the Pseudo-Response Regulator(PRR)family play key roles in regulating flowering.However,their role and molecular mechanism controlling heading date in rice is not very clear.Here,we identified rice OsPRR protein,OsPRR59,which delayed heading under longday conditions.OsPRR59 positively regulates yield by affecting plant height,secondary branches number per panicle,grain number per panicle,seed setting rate,and grain weight per plant.OsPRR59 is expressed in most tissues and its protein is localized to the nucleus.We also found that OsPRR59 directly binds to the promoter of Ehd3 to inhibit its expression.Compared with the WT,osprr59 ehd3 showed a significantly delayed heading phenotype,as did the ehd3 mutant.This was opposite to the phenotype of the osprr59 mutant,confirming that Ehd3 acted downstream of OsPRR59 in regulating rice flowering.Our results identified a direct regulator of Ehd3,and revealed a novel molecular mechanism of clock component OsPRR proteins in regulating heading date and provide a new genetic resource for fine-tuning heading date in rice.展开更多
The present study is conducted to determinate fatty acids(FA) composition in 3 adipose tissues. Subcutaneous and perirenal adipose tissues were prepared from 24 Ningxiang castrated boars and 24 castrated gilts fattene...The present study is conducted to determinate fatty acids(FA) composition in 3 adipose tissues. Subcutaneous and perirenal adipose tissues were prepared from 24 Ningxiang castrated boars and 24 castrated gilts fattened by a traditional diet for 56 d, respectively. The results showed that the FA profile in the 3 adipose tissues(dorsal subcutaneous adipose [DSA], abdominal subcutaneous adipose [ASA], and perirenal adipose [PA]) differed greatly. In boars, the proportions of oleic acid(c18:1 n9 c)(P < 0.05), cis-11-20 c acid(c20:1)(P < 0.05), and a-linolenic acid(c18:3 n3)(P < 0.05) in DSA were the highest among 3 adipose tissues, whereas palmitic acid(c16:0)(P < 0.05) and stearic acid(c18:0)(P < 0.05) in DSA had the lowest proportion. In gilts, cis-11-20 c acid(c20:1)(P < 0.05) in DSA was the highest, while stearic acid(c18:0)(P < 0.05) in subcutaneous adipose was the lowest among these deposits. Overall, the results indicate that from external to inner carcass of boars, the sum of saturated fatty acids(SFA) increase, but the sum of monounsaturated fatty acids(MUFA) decrease, while ASA of gilts have the greatest proportion of MUFA and the lowest SFA. Sex and adipose locations as significant effects on the FA profile are interaction.展开更多
基金supported by the National Natural Science Foundation of China(31771886 and 31771764)China Postdoctoral Science Foundation(2019T120164)Central Public-interest Scientific Institution Basal Research Fund(Y2020YJ10)。
文摘Heading date(or flowering time),an important agronomic trait in crop species,is closely associated with regional adaptation and yield.Members of the Pseudo-Response Regulator(PRR)family play key roles in regulating flowering.However,their role and molecular mechanism controlling heading date in rice is not very clear.Here,we identified rice OsPRR protein,OsPRR59,which delayed heading under longday conditions.OsPRR59 positively regulates yield by affecting plant height,secondary branches number per panicle,grain number per panicle,seed setting rate,and grain weight per plant.OsPRR59 is expressed in most tissues and its protein is localized to the nucleus.We also found that OsPRR59 directly binds to the promoter of Ehd3 to inhibit its expression.Compared with the WT,osprr59 ehd3 showed a significantly delayed heading phenotype,as did the ehd3 mutant.This was opposite to the phenotype of the osprr59 mutant,confirming that Ehd3 acted downstream of OsPRR59 in regulating rice flowering.Our results identified a direct regulator of Ehd3,and revealed a novel molecular mechanism of clock component OsPRR proteins in regulating heading date and provide a new genetic resource for fine-tuning heading date in rice.
基金jointly supported by the Major Project of Hunan Province(2015NK1002)Hunan Provincial Innovation Foundation for Postgraduate(CX2016B277)+1 种基金Key Project of Hunan Provincial Education Department(16A096)Strategic Cooperation Project between Hunan Agricultural University and People's Government of Ningxiang(basic research in Ningxiang pigs)
文摘The present study is conducted to determinate fatty acids(FA) composition in 3 adipose tissues. Subcutaneous and perirenal adipose tissues were prepared from 24 Ningxiang castrated boars and 24 castrated gilts fattened by a traditional diet for 56 d, respectively. The results showed that the FA profile in the 3 adipose tissues(dorsal subcutaneous adipose [DSA], abdominal subcutaneous adipose [ASA], and perirenal adipose [PA]) differed greatly. In boars, the proportions of oleic acid(c18:1 n9 c)(P < 0.05), cis-11-20 c acid(c20:1)(P < 0.05), and a-linolenic acid(c18:3 n3)(P < 0.05) in DSA were the highest among 3 adipose tissues, whereas palmitic acid(c16:0)(P < 0.05) and stearic acid(c18:0)(P < 0.05) in DSA had the lowest proportion. In gilts, cis-11-20 c acid(c20:1)(P < 0.05) in DSA was the highest, while stearic acid(c18:0)(P < 0.05) in subcutaneous adipose was the lowest among these deposits. Overall, the results indicate that from external to inner carcass of boars, the sum of saturated fatty acids(SFA) increase, but the sum of monounsaturated fatty acids(MUFA) decrease, while ASA of gilts have the greatest proportion of MUFA and the lowest SFA. Sex and adipose locations as significant effects on the FA profile are interaction.