The major challenge in photothermal therapy(PTT)is to develop nanocomposites that simultaneously exhibit bioimaging and PTT under a single near-infrared(NIR)irradiation with high therapeutic efficiency.Herein,we prese...The major challenge in photothermal therapy(PTT)is to develop nanocomposites that simultaneously exhibit bioimaging and PTT under a single near-infrared(NIR)irradiation with high therapeutic efficiency.Herein,we present a multifunctional nanocomposite synthesized by linking NaYF_(4):Yb^(3+),Er^(3+)upconversion nanoparticles(UCNPs)with gold nanorods(AuNR)to exhibit fluorescence label-ing,local temperature sensing and photothermal functions simul-taneously with a single NIR laser excitation.The AuNR-NaYF_(4):Yb^(3+),Er^(3+)nanocomposite particles displayed better photothermal prop-erties compared with pure AuNRs or a blend of AuNRs and NaYF_(4):Yb^(3+),Er^(3+)UCNPs.The temperature-dependent upconversion lumi-nescence(UCL)property was used to determine local temperature at the nanocomposite particles,which is useful for selecting appro-priate irradiation dosage for PTT.The therapeutic performance of the nanocomposites in PTT for OML-1 oral cancer cells was deter-mined.For cell labeling,we successfully labeled streptavidin-linked nanocomposite particles on the surface of OML-1 oral cancer using anti-human epidermal growth factor receptor 2(anti-Her2)anti-body.Finally,the nanocomposite particles caused exceptional destruction of cancer cells up to 70%dead cells under 976 nm laser irradiation for only one min at 0.3 W/cm^(2)which is below the maximal permissible exposure of human skin.展开更多
基金This work was supported by Ministry of Science and Technology,Taiwan,under Grant Nos.MOST 107-2923-M-194-001-MY3 and MOST 107-2112-M-194-011-MY3 and Center for Nano Bio-Detection from The Featured Research Areas College Development Plan of National Chung Cheng University.
文摘The major challenge in photothermal therapy(PTT)is to develop nanocomposites that simultaneously exhibit bioimaging and PTT under a single near-infrared(NIR)irradiation with high therapeutic efficiency.Herein,we present a multifunctional nanocomposite synthesized by linking NaYF_(4):Yb^(3+),Er^(3+)upconversion nanoparticles(UCNPs)with gold nanorods(AuNR)to exhibit fluorescence label-ing,local temperature sensing and photothermal functions simul-taneously with a single NIR laser excitation.The AuNR-NaYF_(4):Yb^(3+),Er^(3+)nanocomposite particles displayed better photothermal prop-erties compared with pure AuNRs or a blend of AuNRs and NaYF_(4):Yb^(3+),Er^(3+)UCNPs.The temperature-dependent upconversion lumi-nescence(UCL)property was used to determine local temperature at the nanocomposite particles,which is useful for selecting appro-priate irradiation dosage for PTT.The therapeutic performance of the nanocomposites in PTT for OML-1 oral cancer cells was deter-mined.For cell labeling,we successfully labeled streptavidin-linked nanocomposite particles on the surface of OML-1 oral cancer using anti-human epidermal growth factor receptor 2(anti-Her2)anti-body.Finally,the nanocomposite particles caused exceptional destruction of cancer cells up to 70%dead cells under 976 nm laser irradiation for only one min at 0.3 W/cm^(2)which is below the maximal permissible exposure of human skin.
