Investigation of photodamage by femtosecond laser to cells via gold nanorods

Usually,only focused femtosecond(fs)lasers at near-infrared(NIR)range can induce photo-damage to transparent cells,making it difficult to treat large amount of cells by such optical methods for photostimulation.In this study,we clarify the mechanism of photodamage to cells that are co-cultured with gold nanorods(GNRs)by fs laser.The pulse duration and repetition rate of the fs laser play a key role in cell damage suggesting that the heat accumulation con-tributes to the major part for the cell damage rather than the high peak power which mainly determines the efficiency of multiphoton excitation.We further show that cellular Ca^(2+)can also be released in this scheme,but the process is more sensitive to peak power.Our results can provide a large-scale GNR-mediated photostimulation for cell signaling modulation.

High pulse energy fiber/solid-slab hybrid picosecond pulse system for material processing on polycrystalline diamonds

We demonstrate an all polarization-maintaining(PM) fiber mode-locked laser seeded, hybrid fiber/solid-slab picosecond pulse laser system which outputs 40 μJ, 10 ps pulses at the central wavelength of 1064 nm. The beam quality factors M2 in the unstable and stable directions are 1.35 and 1.31, respectively. 15 μJ picosecond pulses at the central wavelength of 355 nm are generated through third harmonic generation(THG) by using two Li B3 O5(LBO) crystals, in order to get better processing efficiency on polycrystalline diamonds. The high pulse energy and beam quality of these ultraviolet(UV) picosecond pulses are confirmed by latter experiments of material processing on polycrystalline diamonds. This scheme which combines the advantages of the all PM fiber mode-locked laser and the solid-slab amplifier enables compact, robust and chirped pulse amplification-free amplification with high power picosecond pulses.

Hybrid femtosecond laser system based on a Yb:KGW regenerative amplifier for N_P polarization

We report a hybrid femtosecond laser system based on a femtosecond Yb-doped fiber laser and a Yb-doped potassium gadolinium tungstate(Yb:KGW) regenerative amplifier. To match the central wavelength of the seed source, a Yb:KGW crystal is used in the regenerative amplifier for N_p polarization. We study and optimize the dynamics of nonlinear amplification to alleviate the gain narrowing effect. With optimization, the system can output 270 fs pulses with 21 μJ pulse energy at a 60 kHz repetition rate.

Nonlinearity optimization of dissipative-soliton fiber laser for generation of pulses with 350 kW peak power

We demonstrate a nonlinearity optimization method by altering distribution of passive fibers in a dissipative-soliton mode-locked fiber laser to level up output parameters. In the numerical simulation, we found that the passive fiber segment after gain fiber characterizes the highest average B-integral among fiber segments. By reducing the length of this fiber section and keeping the total passive fiber length as constant, the output pulse energy can be effectively scaled up while maintaining a short dechirped pulse duration, resulting in boosting peak power. With this method, 37-n J pulses are generated from a dissipative-soliton mode-locked cladding pumped ytterbium-doped single-mode fiber laser in the experiment. The pulse can be dechirped to 66 fs with 350 k W peak power. Moreover, the pulse pedestal is suppressed by a vector-dispersion compressor.