称取复方咪康唑乳样品约1g,加入乙醇25 mL,加热溶解后冷却,用乙醇定容至50mL,摇匀,冷冻1h后迅速用滤纸过滤,取续滤液放置至室温后,采用Agilent Eclipse Plus C18色谱柱(250mm×4.6mm,5μm)进行分离,以甲醇、乙腈和30g·L^(-1)...称取复方咪康唑乳样品约1g,加入乙醇25 mL,加热溶解后冷却,用乙醇定容至50mL,摇匀,冷冻1h后迅速用滤纸过滤,取续滤液放置至室温后,采用Agilent Eclipse Plus C18色谱柱(250mm×4.6mm,5μm)进行分离,以甲醇、乙腈和30g·L^(-1)乙酸铵溶液的混合液为流动相进行梯度洗脱,乳酸依沙吖啶、曲安奈德和硝酸咪康唑的检测波长分别为270,240,230nm。乳酸依沙吖啶、曲安奈德和硝酸咪康唑的线性范围分别为40～180,40～200,800～401mg·L^(-1),检出限(3S/N)分别为0.75,1.06,2.18mg·L^(-1)。方法用于测定复方咪康唑乳样品中的乳酸依沙吖啶、曲安奈德和硝酸咪康唑,测定值与标示值一致,加标回收率在96.1%～104%之间,测定值的相对标准偏差(n=6)小于1.0%。展开更多
The juice leaked from broken phloem of Paulownia elongate S.Y.Hu has been used to cure acute inflammation resulted from stung injury by poisonous insects for a long time in China,but its potential mechanism remains un...The juice leaked from broken phloem of Paulownia elongate S.Y.Hu has been used to cure acute inflammation resulted from stung injury by poisonous insects for a long time in China,but its potential mechanism remains unclear.The present study was designed to evaluate anti-inflammatory activity and mechanism of total flavonoids from the phloem of P.elongate S.Y.Hu in RAW264.7 cells.Lipopolysaccharide(LPS)-induced nitric oxide(NO)was measured by Griess and mRNA of pro-inflammatory mediators was analyzed by q-PCR.Cell viability was measured using cell counting kit(CCK)-8 assay.The protein level was analyzed by Western blot.The results showed that the total flavonoids of P.elongate significantly inhibited the production of the pro-inflammatory mediators such as NO,interleukin(IL)-1β and interleukin(IL)-6 in LPS-stimulated RAW264.7 cells.Total flavonoids of P.elongate exerted potential anti-inflammatory activity through the regulation of several signaling pathways.Total flavonoids of P.elongate inhibited JAK/STAT by blocking JAK2 and STAT3 phosphorylation levels.This is the first report on anti-inflammatory activity of total flavonoids from the phloem of P.elongate,which suggests that the total flavonoids of P.elongate may have great potential for the development of anti-inflammatory drug to treat inflammatory disorders.展开更多
文摘称取复方咪康唑乳样品约1g,加入乙醇25 mL,加热溶解后冷却,用乙醇定容至50mL,摇匀,冷冻1h后迅速用滤纸过滤,取续滤液放置至室温后,采用Agilent Eclipse Plus C18色谱柱(250mm×4.6mm,5μm)进行分离,以甲醇、乙腈和30g·L^(-1)乙酸铵溶液的混合液为流动相进行梯度洗脱,乳酸依沙吖啶、曲安奈德和硝酸咪康唑的检测波长分别为270,240,230nm。乳酸依沙吖啶、曲安奈德和硝酸咪康唑的线性范围分别为40～180,40～200,800～401mg·L^(-1),检出限(3S/N)分别为0.75,1.06,2.18mg·L^(-1)。方法用于测定复方咪康唑乳样品中的乳酸依沙吖啶、曲安奈德和硝酸咪康唑,测定值与标示值一致,加标回收率在96.1%～104%之间,测定值的相对标准偏差(n=6)小于1.0%。
基金Henan Provincial Department of Science&Technology(172102310165)Henan Provincial Department of Education(21B350001).
文摘The juice leaked from broken phloem of Paulownia elongate S.Y.Hu has been used to cure acute inflammation resulted from stung injury by poisonous insects for a long time in China,but its potential mechanism remains unclear.The present study was designed to evaluate anti-inflammatory activity and mechanism of total flavonoids from the phloem of P.elongate S.Y.Hu in RAW264.7 cells.Lipopolysaccharide(LPS)-induced nitric oxide(NO)was measured by Griess and mRNA of pro-inflammatory mediators was analyzed by q-PCR.Cell viability was measured using cell counting kit(CCK)-8 assay.The protein level was analyzed by Western blot.The results showed that the total flavonoids of P.elongate significantly inhibited the production of the pro-inflammatory mediators such as NO,interleukin(IL)-1β and interleukin(IL)-6 in LPS-stimulated RAW264.7 cells.Total flavonoids of P.elongate exerted potential anti-inflammatory activity through the regulation of several signaling pathways.Total flavonoids of P.elongate inhibited JAK/STAT by blocking JAK2 and STAT3 phosphorylation levels.This is the first report on anti-inflammatory activity of total flavonoids from the phloem of P.elongate,which suggests that the total flavonoids of P.elongate may have great potential for the development of anti-inflammatory drug to treat inflammatory disorders.
