Eukaryotic DNA replication is tightly restricted to onlyonce per cell cycle in order to maintain genome stability.Cells use multiple mechanisms to control the assemblyof the prereplication complex (pre-RC), a process ...Eukaryotic DNA replication is tightly restricted to onlyonce per cell cycle in order to maintain genome stability.Cells use multiple mechanisms to control the assemblyof the prereplication complex (pre-RC), a process knownas replication licensing. This review focuses on theregulation of replication licensing by posttranslationalmodifications of the licensing factors, including phosphorylation, ubiquitylation and acetylation. These modifications are critical in establishing the pre-RCcomplexes as well as preventing rereplication in each cellcycle. The relationship between rereplication and diseases, including cancer and virus infection, is discussedas well.展开更多
Salt stress is a major abiotic stress which severely hinders crop production.However,the regulatory network controlling tomato resistance to salt remains unclear.Here,we found that the tomato WRKY transcription factor...Salt stress is a major abiotic stress which severely hinders crop production.However,the regulatory network controlling tomato resistance to salt remains unclear.Here,we found that the tomato WRKY transcription factor WRKY57 acted as a negative regulator in salt stress response by directly attenuating the transcription of salt-responsive genes(Sl RD29B and Sl DREB2)and an ion homeostasis gene(Sl SOS1).We further identified two VQ-motif containing proteins Sl VQ16 and Sl VQ21as Sl WRKY57-interacting proteins.Sl VQ16 positively,while Sl VQ21 negatively modulated tomato resistance to salt stress.Sl VQ16 and Sl VQ21 competitively interacted with Sl WRKY57 and antagonistically regulated the transcriptional repression activity of Sl WRKY57.Additionally,the Sl WRKY57-Sl VQ21/Sl VQ16 module was involved in the pathway of phytohormone jasmonates(JAs)by interacting with JA repressors JA-ZIM domain(JAZ)proteins.These results provide new insights into how the Sl WRKY57-Sl VQ21/Sl VQ16 module finely tunes tomato salt tolerance.展开更多
文摘Eukaryotic DNA replication is tightly restricted to onlyonce per cell cycle in order to maintain genome stability.Cells use multiple mechanisms to control the assemblyof the prereplication complex (pre-RC), a process knownas replication licensing. This review focuses on theregulation of replication licensing by posttranslationalmodifications of the licensing factors, including phosphorylation, ubiquitylation and acetylation. These modifications are critical in establishing the pre-RCcomplexes as well as preventing rereplication in each cellcycle. The relationship between rereplication and diseases, including cancer and virus infection, is discussedas well.
基金supported by the Project of Cultivation for young top-notch Talents of Beijing Municipal Institutions (Grant No.BPHR202203099)。
文摘Salt stress is a major abiotic stress which severely hinders crop production.However,the regulatory network controlling tomato resistance to salt remains unclear.Here,we found that the tomato WRKY transcription factor WRKY57 acted as a negative regulator in salt stress response by directly attenuating the transcription of salt-responsive genes(Sl RD29B and Sl DREB2)and an ion homeostasis gene(Sl SOS1).We further identified two VQ-motif containing proteins Sl VQ16 and Sl VQ21as Sl WRKY57-interacting proteins.Sl VQ16 positively,while Sl VQ21 negatively modulated tomato resistance to salt stress.Sl VQ16 and Sl VQ21 competitively interacted with Sl WRKY57 and antagonistically regulated the transcriptional repression activity of Sl WRKY57.Additionally,the Sl WRKY57-Sl VQ21/Sl VQ16 module was involved in the pathway of phytohormone jasmonates(JAs)by interacting with JA repressors JA-ZIM domain(JAZ)proteins.These results provide new insights into how the Sl WRKY57-Sl VQ21/Sl VQ16 module finely tunes tomato salt tolerance.
