Background: The growth rate often varies among individual broilers of the same breed under a common management condition. To investigate whether a variation in the growth rate is associated with a difference in hormo...Background: The growth rate often varies among individual broilers of the same breed under a common management condition. To investigate whether a variation in the growth rate is associated with a difference in hormone levels and myogenic gene expression profile in broilers, a feeding trial was conducted with 10,000 newly hatched Ross 308 chicks in a commercial production facility under standard management. At 38 d of age,30 fast-, 30 medium-, and 30 slow-growing broilers were selected among 600 healthy male individuals. The levels of insulin-like growth factor-1(IGF-1), triiodothyronine(T3), thyroxine(T4), and growth hormone in the serum or breast muscle were assayed by ELISA or RIA kits, and the expression levels of several representative pro-and anti-myogenic genes in the breast muscle were also measured by real-time PCR.Results: Results showed that both absolute and relative weights of the breast muscle were in linear positive correlations with the body weight of broilers(P 〈 0.001). Fast-growing broilers had higher concentrations of IGF-1 than slow-growing broilers(P 〈 0.05) in both the serum and breast muscle. The serum concentration of T3 was significantly higher in fast-growing birds than in slow-growing birds(P 〈 0.05). However, no difference was observed in growth hormone or T4 concentration among three groups of birds. Additionally, a decreased expression of an anti-myogenic gene(myostatin) and increased expressions of pro-myogenic genes such as myogenic differentiation factor 1, myogenin, muscle regulatory factor 4, myogenic factor 5, IGF-1, and myocyte enhancer factor 2B, C, and D were observed in fast-growing broilers(P 〈 0.05), relative to slow-growing broilers.Conclusions: Collectively, these findings suggested that the growth rate is linked to the hormone and myogenic gene expression levels in broiler chickens. Some of these parameters such as serum concentrations of IGF-1 and T3 could be employed to breed for enhanced growth.展开更多
Autophagy is a highly conserved degradation process in which intracellular components are engulfed into a double membrane structure,known as the autophagosomes,and then delivered to lysosomes/vacuoles for degradation(...Autophagy is a highly conserved degradation process in which intracellular components are engulfed into a double membrane structure,known as the autophagosomes,and then delivered to lysosomes/vacuoles for degradation(Ohsumi,2014).Recently,autophagy has been found to be responsible for maintaining glucose homeostasis in adult animals(Karsli-Uzunbas et al.,2014).However,the molecular mechanism underlying energy deprivation-induced autophagy remains relatively unclear.展开更多
基金supported by the Special Fund for Public Projects of Zhejiang Province(2016C32073)International Cooperation Program of Zhejiang Academy of Agricultural Sciences
文摘Background: The growth rate often varies among individual broilers of the same breed under a common management condition. To investigate whether a variation in the growth rate is associated with a difference in hormone levels and myogenic gene expression profile in broilers, a feeding trial was conducted with 10,000 newly hatched Ross 308 chicks in a commercial production facility under standard management. At 38 d of age,30 fast-, 30 medium-, and 30 slow-growing broilers were selected among 600 healthy male individuals. The levels of insulin-like growth factor-1(IGF-1), triiodothyronine(T3), thyroxine(T4), and growth hormone in the serum or breast muscle were assayed by ELISA or RIA kits, and the expression levels of several representative pro-and anti-myogenic genes in the breast muscle were also measured by real-time PCR.Results: Results showed that both absolute and relative weights of the breast muscle were in linear positive correlations with the body weight of broilers(P 〈 0.001). Fast-growing broilers had higher concentrations of IGF-1 than slow-growing broilers(P 〈 0.05) in both the serum and breast muscle. The serum concentration of T3 was significantly higher in fast-growing birds than in slow-growing birds(P 〈 0.05). However, no difference was observed in growth hormone or T4 concentration among three groups of birds. Additionally, a decreased expression of an anti-myogenic gene(myostatin) and increased expressions of pro-myogenic genes such as myogenic differentiation factor 1, myogenin, muscle regulatory factor 4, myogenic factor 5, IGF-1, and myocyte enhancer factor 2B, C, and D were observed in fast-growing broilers(P 〈 0.05), relative to slow-growing broilers.Conclusions: Collectively, these findings suggested that the growth rate is linked to the hormone and myogenic gene expression levels in broiler chickens. Some of these parameters such as serum concentrations of IGF-1 and T3 could be employed to breed for enhanced growth.
基金supported by the National Natural Science Foundation of China(32070739,31771528,and 91754107)Zhejiang Provincial Natural Science Foundation of China(LR21C070001)+3 种基金the National Basic Research Program of China(2017YFA0503402)to C.YiNatural Science Foundation for Young Scientists of China(81800085)Natural Science Foundation of Hubei Province,China(ZRMS2020002147)to J.TongZhejiang Key R&D Program of China(2019C02030)to L.Zhang。
文摘Autophagy is a highly conserved degradation process in which intracellular components are engulfed into a double membrane structure,known as the autophagosomes,and then delivered to lysosomes/vacuoles for degradation(Ohsumi,2014).Recently,autophagy has been found to be responsible for maintaining glucose homeostasis in adult animals(Karsli-Uzunbas et al.,2014).However,the molecular mechanism underlying energy deprivation-induced autophagy remains relatively unclear.