The lack of capability to quantify oxygen metabolism noninvasively impedes both fundamental investigation and clinical diagnosis of a wide spectrum of diseases including all the major blinding diseases such as age-rel...The lack of capability to quantify oxygen metabolism noninvasively impedes both fundamental investigation and clinical diagnosis of a wide spectrum of diseases including all the major blinding diseases such as age-related macular degeneration,diabetic retinopathy,and glaucoma.Using visible light optical coherence tomography(vis-OCT),we demonstrated accurate and robust measurement of retinal oxygen metabolic rate(rMRO2)noninvasively in rat eyes.We continuously monitored the regulatory response of oxygen consumption to a progressive hypoxic challenge.We found that both oxygen delivery,and rMRO2 increased from the highly regulated retinal circulation(RC)under hypoxia,by 0.2860.08 μL min(^-1)(p,0.001),and 0.2060.04 μL min(^-1)(p,0.001)per 100 mmHg systemic pO2 reduction,respectively.The increased oxygen extraction compensated for the deficient oxygen supply from the poorly regulated choroidal circulation.Results from an oxygen diffusion model based on previous oxygen electrode measurements corroborated our in vivo observations.We believe that vis-OCT has the potential to reveal the fundamental role of oxygen metabolism in various retinal diseases.展开更多
基金We would like to acknowledge the generous financial support from the NIH(Grant Nos 1R01EY019951 and 1R24EY022883)NSF(Grant Nos CBET-1055379 and CBET-1066776)+1 种基金Wenzhong Liu is supported by a HHMI graduate student fellowshipJi Yi is supported by a Seed Grant from the Illinois Society for Blindness Prevention and a post-doctoral fellowship award from the Juvenile Diabetes Research Foundation(JDRF).
文摘The lack of capability to quantify oxygen metabolism noninvasively impedes both fundamental investigation and clinical diagnosis of a wide spectrum of diseases including all the major blinding diseases such as age-related macular degeneration,diabetic retinopathy,and glaucoma.Using visible light optical coherence tomography(vis-OCT),we demonstrated accurate and robust measurement of retinal oxygen metabolic rate(rMRO2)noninvasively in rat eyes.We continuously monitored the regulatory response of oxygen consumption to a progressive hypoxic challenge.We found that both oxygen delivery,and rMRO2 increased from the highly regulated retinal circulation(RC)under hypoxia,by 0.2860.08 μL min(^-1)(p,0.001),and 0.2060.04 μL min(^-1)(p,0.001)per 100 mmHg systemic pO2 reduction,respectively.The increased oxygen extraction compensated for the deficient oxygen supply from the poorly regulated choroidal circulation.Results from an oxygen diffusion model based on previous oxygen electrode measurements corroborated our in vivo observations.We believe that vis-OCT has the potential to reveal the fundamental role of oxygen metabolism in various retinal diseases.
