A modified separation method has been developed for determinating polychlorinated biphenyl congeners in environmental samples. Direct treatment of extract with concentrated HzSO4 was employed in the first step for rem...A modified separation method has been developed for determinating polychlorinated biphenyl congeners in environmental samples. Direct treatment of extract with concentrated HzSO4 was employed in the first step for removal of lipids and other interfering substances, then a joint column of alumina-silica gel(Ag+) was applied to separate PCBs fraction from HCH, DDT and its analogs. After this separation, the PCBs fraction was analyzed by capillary gas chromatography with ECD detector and confirmed by GC/MS. The recoveries of individual congeners in Aroclor 1254 through the separation are about 79%-84%. The method is very efficient and useful for determination of trace amount of PCB congeners in environmental samples.展开更多
ToxChip is the molecular biological technology which has been developed based on genome and DNA microarray technologies. It will be able to make the scientists evaluate the toxicity of extraneous toxicants at the mole...ToxChip is the molecular biological technology which has been developed based on genome and DNA microarray technologies. It will be able to make the scientists evaluate the toxicity of extraneous toxicants at the molecular level. ToxChip was successfully developed by a group of the National Institute of Environmental Health Sciences (NIEHS) researchers in the United States in 1999[1]. The technology bears revolutionary significance on the traditional toxicology. It foretells the epoch in which DNA effects of environmental dangers and toxicants can be made certain rapidly and efficiently to fall. ToxChip will pioneer novel approaches for medicine, environmental and ecological toxicologies.展开更多
文摘A modified separation method has been developed for determinating polychlorinated biphenyl congeners in environmental samples. Direct treatment of extract with concentrated HzSO4 was employed in the first step for removal of lipids and other interfering substances, then a joint column of alumina-silica gel(Ag+) was applied to separate PCBs fraction from HCH, DDT and its analogs. After this separation, the PCBs fraction was analyzed by capillary gas chromatography with ECD detector and confirmed by GC/MS. The recoveries of individual congeners in Aroclor 1254 through the separation are about 79%-84%. The method is very efficient and useful for determination of trace amount of PCB congeners in environmental samples.
基金This work was supported by the Key Project during the Ninth Five-year Plan of the Chinese Academy of Sciences (Grant No. KJ952-J1-520) RCEES9902, 9906 and the National Natural Science Foundation of China (Grant No. 29977026).
文摘ToxChip is the molecular biological technology which has been developed based on genome and DNA microarray technologies. It will be able to make the scientists evaluate the toxicity of extraneous toxicants at the molecular level. ToxChip was successfully developed by a group of the National Institute of Environmental Health Sciences (NIEHS) researchers in the United States in 1999[1]. The technology bears revolutionary significance on the traditional toxicology. It foretells the epoch in which DNA effects of environmental dangers and toxicants can be made certain rapidly and efficiently to fall. ToxChip will pioneer novel approaches for medicine, environmental and ecological toxicologies.
