OBJECTIVE:To observe the intervention of Chushizi(Fructus Broussonetiae)(CSZ)on drug-induced liver injury(DILI)in rats,as well as indicators of liver function,serum levels of inflammatory cytokines,and expression of p...OBJECTIVE:To observe the intervention of Chushizi(Fructus Broussonetiae)(CSZ)on drug-induced liver injury(DILI)in rats,as well as indicators of liver function,serum levels of inflammatory cytokines,and expression of proteins and m RNA associated with toll-like receptor 3(TLR3)and the signal transducer and activator of transcription 3(STAT3)pathway in the liver[TLR3,janus protein tyrosine kinase 2(JAK2),c-jun,c-fos,c-Jun N-terminal kinase 2(JNK2),and STAT3].METHODS:Forty specified pathogen free grade Sprague-Dawley rats were randomly divided into the control group,the model group,the silybin group and the CSZ group.Rats were given acetaminophen(APAP)to trigger DILI.Histopathology of the liver was observed by hematoxylin-eosin staining.The levels of alanine aminotransferase(ALT),aspartate transaminase(AST),direct bilirubin(DBIL),and total bilirubin(TBIL)in serum were detected by a semi-automatic biochemical instrument.Content of tumor necrosis factor alpha(TNF-α),interleukin(IL)-6,IL-13,and IL-22 in serum were detected by the enzyme-linked immunosorbent assay,the expression of TLR3,phosphorylation of JAK2(p-JAK2),while c-jun and c-fos proteins in the liver were determined by immunohistochemistry;expression of JNK2,and STAT3 in the liver were assayed by Western blot and real-time quantitative polymerase chain reaction.P-JNK2 and p-STAT3 in the liver were assayed by Western blot.RESULTS:After treatment,the activity of ALT,AST,and concentrations of TBIL,DBIL,TNF-α,IL-6,as well as IL-13 in serum,were lower than those in the model group,and expression of p-JAK2,TLR3,c-jun,c-fos,p-STAT3,and p-JNK2 could be downregulated.CONCLUSION:Our findings suggest that CSZ is a valid medicine to alleviate APAP-induced DILI,while its partial mechanism may regulate the TLR3/JNK/c-jun/c-fos/JAK/STAT3 pathway.展开更多
目的 评价柴金化瘀方联合内镜微创保胆取息肉术治疗胆囊息肉样变的疗效。方法 将符合入选标准的胆囊息肉样变患者91例采用随机数字表法分为观察组46例与对照组45例。对照组给予内镜微创保胆取息肉术治疗，观察组在对照组基础上加服柴金...目的 评价柴金化瘀方联合内镜微创保胆取息肉术治疗胆囊息肉样变的疗效。方法 将符合入选标准的胆囊息肉样变患者91例采用随机数字表法分为观察组46例与对照组45例。对照组给予内镜微创保胆取息肉术治疗，观察组在对照组基础上加服柴金化瘀方治疗。2组均治疗24周。采用中医症状积分量表对心下痞满、右胁胀满、嗳气症状进行评分，采用简明健康状况调查表（36-item Short Form Health Survey, SF-36）评价患者生活质量，记录术后并发症发生情况，评价临床疗效。结果 观察组总有效率为91.3%（42/46）、对照组为88.9%（40/45），2组比较差异无统计学意义（χ2=0.149，P=0.592）。治疗后，症状积分量表中，观察组心下痞满、右胁胀痛、嗳气评分均低于对照组（t值分别为2.431、3.560、2.891，P值均＜0.05）；SF-36量表中，观察组精神健康、社会功能、躯体疼痛、生理职能、情感职能、活力、生理功能、总体健康评分均高于对照组（t值分别为2.342、2.831、2.765、2.545、2.485、2.650、2.582、2.325，P值均＜0.05）。观察组术后并发症发生率为2.2%（1/46）、对照组为17.8%（8/45），2组比较差异有统计学意义（χ2=6.331，P=0.014）。结论 柴金化瘀方联合微创保胆术取息肉术可提高胆囊息肉样变患者的生活质量，有效减轻术后并发症，提高临床疗效。展开更多
OBJECTIVE: To investigate the effect of Danggui Buxue Tang(DBT), a decoction from Traditional Chinese Medicine, on bleomycin-induced pulmonary fibrosis in rats, and to propose the possible underlying mechanism.METHODS...OBJECTIVE: To investigate the effect of Danggui Buxue Tang(DBT), a decoction from Traditional Chinese Medicine, on bleomycin-induced pulmonary fibrosis in rats, and to propose the possible underlying mechanism.METHODS: Forty male Sprague-Dawley rats were randomly divided into sham group, model group,prednisone group and DBT group. Pulmonary fibrosis rat model was established by intratracheal injection with bleomycin. Body weight and lung index were monitored. Histopathologic examination and collagen deposition were determined using Hematoxylin and eosin(HE) and Masson's trichrome staining. Immunohistochemistry staining was applied to observe the expression of alpha-smooth muscle actin(α-SMA). m RNA expression of α-SMA,collagen Ⅰ and collagen Ⅲ were measured by realtime fluorescence quantitative PCR(RT-q PCR). Inflammatory cytokines, including tumor necrosis factor alpha(TNF-α), interleukin-6(IL-6) and IL-1β in serum were detected by Enzyme-linked immunosorbent assay. Alkali hydrolysis method was conducted to investigate the content of hydroxyproline(HYP). Transforming growth factor-β1(TGF-β1),Smad3 and plasminogen activator inhibitor-1(PAI-1) protein level were examined by Western blot assay.RESULTS: DBT significantly reduced the severity of bleomycin-induced pulmonary fibrosis and inflammation as indicated by minimizing the lost of weight, and by lowering the levels of lung index, inflammation score, Ashcroft score, collagen volume fraction(%), HYP, α-SMA, collagen Ⅰ, collagen Ⅲ,TNF-α, IL-6, IL-1β, TGF-β1, Smad3 and PAI-1, consistent with the effect of prednisone.CONCLUSION: Our findings suggest that DBT is able to ameliorate the pulmonary fibrosis, the possible mechanism may involve inhibition of pulmonary inflammation and collagen deposition, possibly via suppressing TGF-β1/Smad3/PAI-1 signaling pathway.展开更多
基金Supported by the National Natural Science Foundation of China(Study on the Compatibility Relationship and Mechanism of Vinegar Kansui and Roasted Licorice Based on the Theory of Medicine Syndrome,No.81503268)the Top Program of Science and Technology Research Youth in Colleges and Universities of Hebei Province(Study on the Preventive and Therapeutic Effect and Mechanism of Jiedu Hugan Recipe on Drug-Induced Liver Injury,No.BJ2016038)+1 种基金the Central Finance Public Health Project 2017the General Survey of Traditional Chinese Medicine Resources(No.Z135080000022)。
文摘OBJECTIVE:To observe the intervention of Chushizi(Fructus Broussonetiae)(CSZ)on drug-induced liver injury(DILI)in rats,as well as indicators of liver function,serum levels of inflammatory cytokines,and expression of proteins and m RNA associated with toll-like receptor 3(TLR3)and the signal transducer and activator of transcription 3(STAT3)pathway in the liver[TLR3,janus protein tyrosine kinase 2(JAK2),c-jun,c-fos,c-Jun N-terminal kinase 2(JNK2),and STAT3].METHODS:Forty specified pathogen free grade Sprague-Dawley rats were randomly divided into the control group,the model group,the silybin group and the CSZ group.Rats were given acetaminophen(APAP)to trigger DILI.Histopathology of the liver was observed by hematoxylin-eosin staining.The levels of alanine aminotransferase(ALT),aspartate transaminase(AST),direct bilirubin(DBIL),and total bilirubin(TBIL)in serum were detected by a semi-automatic biochemical instrument.Content of tumor necrosis factor alpha(TNF-α),interleukin(IL)-6,IL-13,and IL-22 in serum were detected by the enzyme-linked immunosorbent assay,the expression of TLR3,phosphorylation of JAK2(p-JAK2),while c-jun and c-fos proteins in the liver were determined by immunohistochemistry;expression of JNK2,and STAT3 in the liver were assayed by Western blot and real-time quantitative polymerase chain reaction.P-JNK2 and p-STAT3 in the liver were assayed by Western blot.RESULTS:After treatment,the activity of ALT,AST,and concentrations of TBIL,DBIL,TNF-α,IL-6,as well as IL-13 in serum,were lower than those in the model group,and expression of p-JAK2,TLR3,c-jun,c-fos,p-STAT3,and p-JNK2 could be downregulated.CONCLUSION:Our findings suggest that CSZ is a valid medicine to alleviate APAP-induced DILI,while its partial mechanism may regulate the TLR3/JNK/c-jun/c-fos/JAK/STAT3 pathway.
文摘目的 评价柴金化瘀方联合内镜微创保胆取息肉术治疗胆囊息肉样变的疗效。方法 将符合入选标准的胆囊息肉样变患者91例采用随机数字表法分为观察组46例与对照组45例。对照组给予内镜微创保胆取息肉术治疗，观察组在对照组基础上加服柴金化瘀方治疗。2组均治疗24周。采用中医症状积分量表对心下痞满、右胁胀满、嗳气症状进行评分，采用简明健康状况调查表（36-item Short Form Health Survey, SF-36）评价患者生活质量，记录术后并发症发生情况，评价临床疗效。结果 观察组总有效率为91.3%（42/46）、对照组为88.9%（40/45），2组比较差异无统计学意义（χ2=0.149，P=0.592）。治疗后，症状积分量表中，观察组心下痞满、右胁胀痛、嗳气评分均低于对照组（t值分别为2.431、3.560、2.891，P值均＜0.05）；SF-36量表中，观察组精神健康、社会功能、躯体疼痛、生理职能、情感职能、活力、生理功能、总体健康评分均高于对照组（t值分别为2.342、2.831、2.765、2.545、2.485、2.650、2.582、2.325，P值均＜0.05）。观察组术后并发症发生率为2.2%（1/46）、对照组为17.8%（8/45），2组比较差异有统计学意义（χ2=6.331，P=0.014）。结论 柴金化瘀方联合微创保胆术取息肉术可提高胆囊息肉样变患者的生活质量，有效减轻术后并发症，提高临床疗效。
基金Supported by the Government Funded Clinical Medicine Eexcellent Talent Training and Basic Research Project PlanNatural Science Foundation of Hebei(No.H2019423092)+2 种基金Higher Education Science and Technology Research Project of Hebei(No.ZD2016056)Postgraduate Innovation Ability Development Project of Hebei Education Department(No.CXZZBS2019159)Basic Research Business Expenses of Provincial Universities of Hebei University of Chinese Medicine Project of Excellent Student Research Capacity Improvement(No.YXZ2019001)。
文摘OBJECTIVE: To investigate the effect of Danggui Buxue Tang(DBT), a decoction from Traditional Chinese Medicine, on bleomycin-induced pulmonary fibrosis in rats, and to propose the possible underlying mechanism.METHODS: Forty male Sprague-Dawley rats were randomly divided into sham group, model group,prednisone group and DBT group. Pulmonary fibrosis rat model was established by intratracheal injection with bleomycin. Body weight and lung index were monitored. Histopathologic examination and collagen deposition were determined using Hematoxylin and eosin(HE) and Masson's trichrome staining. Immunohistochemistry staining was applied to observe the expression of alpha-smooth muscle actin(α-SMA). m RNA expression of α-SMA,collagen Ⅰ and collagen Ⅲ were measured by realtime fluorescence quantitative PCR(RT-q PCR). Inflammatory cytokines, including tumor necrosis factor alpha(TNF-α), interleukin-6(IL-6) and IL-1β in serum were detected by Enzyme-linked immunosorbent assay. Alkali hydrolysis method was conducted to investigate the content of hydroxyproline(HYP). Transforming growth factor-β1(TGF-β1),Smad3 and plasminogen activator inhibitor-1(PAI-1) protein level were examined by Western blot assay.RESULTS: DBT significantly reduced the severity of bleomycin-induced pulmonary fibrosis and inflammation as indicated by minimizing the lost of weight, and by lowering the levels of lung index, inflammation score, Ashcroft score, collagen volume fraction(%), HYP, α-SMA, collagen Ⅰ, collagen Ⅲ,TNF-α, IL-6, IL-1β, TGF-β1, Smad3 and PAI-1, consistent with the effect of prednisone.CONCLUSION: Our findings suggest that DBT is able to ameliorate the pulmonary fibrosis, the possible mechanism may involve inhibition of pulmonary inflammation and collagen deposition, possibly via suppressing TGF-β1/Smad3/PAI-1 signaling pathway.
文摘目的研究老十针临床应用疾病谱,为推动该针法在临床的应用及深入研究提供佐证。方法检索中国学术期刊全文数据库(CNKI)、中文科技期刊全文数据库(VIP)、万方数据库和中国生物医学文献服务系统(SinoMed)、PubMed、Cochrane Library、Web of Science建库至2021年10月老十针相关研究文献,运用文献计量学方法对其文献特征信息(文献发表年份、期刊来源、著者分布、干预措施、研究疾病谱、研究类型、疗效评价、被引量等)进行定量分析。结果共纳入文献81篇,发表于44种期刊上,内容以随机对照临床研究为主,疗法以老十针为基础方针刺治疗或针药二联综合疗法为主。从疾病谱分析,老十针应用范围集中于消化系统疾病(66.67%)与神经系统(17.28%),也涉及循环系统及精神和行为障碍类疾病等其他7类系统疾病。结论老十针相关的临床研究较少,相应疾病谱较窄,基础研究基本缺失,需加大对传统针灸穴方的临床应用探索,进一步规范临床试验设计、提高研究质量并加强其机制研究。
