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中华鳖dazl基因克隆及在生殖细胞中的表达 被引量:2
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作者 唐舟凯 张飘逸 +4 位作者 储张杰 朱新平 李伟 吴栩灵 徐红艳 《水产学报》 CAS CSCD 北大核心 2019年第2期400-409,共10页
为探索龟鳖类生殖细胞的发育分化机制,实验通过特异性引物克隆了中华鳖dazl基因的cDNA片段,长1 007 bp,其中包括5′端非编码区197 bp,3′端非编码区33 bp,开放阅读框777 bp,编码258个氨基酸。氨基酸序列比对显示其与西部锦龟Dazl同源性... 为探索龟鳖类生殖细胞的发育分化机制,实验通过特异性引物克隆了中华鳖dazl基因的cDNA片段,长1 007 bp,其中包括5′端非编码区197 bp,3′端非编码区33 bp,开放阅读框777 bp,编码258个氨基酸。氨基酸序列比对显示其与西部锦龟Dazl同源性最高,达96%;与小鼠Dazl同源性达75%。荧光定量PCR分析结果显示,中华鳖dazl mRNA主要在精巢和卵巢中表达,在体细胞组织中仅检测到微量表达。冰冻切片原位杂交结果显示,中华鳖dazl mRNA在生殖细胞中特异表达,且在不同时期的生殖细胞中呈动态表达模式。在精巢中,中华鳖dazl mRNA在初级和次级精母细胞中表达最强,在精原干细胞中表达水平次之,在精子细胞中未检测到信号;在卵巢中,中华鳖dazl mRNA信号在初级卵母细胞胞质中均匀分布且在最早期的初级卵母细胞中信号最强,随着卵母细胞的增大,信号逐渐聚集并逐渐减弱。研究表明,dazl基因可能对中华鳖两性生殖细胞的发生具有重要的调控作用。 展开更多
关键词 中华鳖 DAZL 生殖细胞 精子发生 卵子发生
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Evaluation of Nutrient Components and Nutritive Quality of Larimichthys crocea(Big Yellow Croaker)in Different Aquaculture Models
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作者 Zhong Aihua chu zhangjie +1 位作者 Dai Luyi Wang Xiaojun 《Animal Husbandry and Feed Science》 CAS 2014年第6期296-299,318,共5页
To ascertain the nutrient components and nutritive quality of the flesh of big yellow croaker in three culture conditions ( traditional cage, offshore cage and cage-free), basic nutritional components,amino acid,fat... To ascertain the nutrient components and nutritive quality of the flesh of big yellow croaker in three culture conditions ( traditional cage, offshore cage and cage-free), basic nutritional components,amino acid,fatty acid and mineral elements were determined. The results indicated that crude protein in flesh of the big yellow croaker in cage-free culture was higher than that in offshore cage and much higher than that in traditional cage ( P 〈0.05). Crude fat of the croaker cul- tured in the traditional cage was twice as high as that in cage-free culture, while that in the offshore cage was in the middle. Proline content in the cage-free culture was much higher than that in the offshore cage, and also than that in the traditional cage (P 〈 0.05 ). There was no significant difference in the content of alanine, methionine and tryptophan ( P 〈 0.05). Contents of other amino acids had no significant difference between the cage-free culture and offshore cage, but were much lower in the traditional cage (P 〈 0.05 ). Top six fatty acids were 9-Hexadecenoic acid, palmitic acid,9-Octadecenoic acid, Octadecauoic acid, DHA and EPA. The palmitic acid content was the highest in cage-free culture and in traditional cage, 9-Octadeeenoic acid content was the highest in offshore cage. Content of unsaturat- ed fatty acids in cage-free culture, offshore cage and traditional cage was 63.60,66.32,57.67, respectively, and polyunsaturated fatty acid was 29.10, 28.57, and 24.40. Content of DHA in cage-free culture was significantly higher than that in the offshore and traditional cage. Content of zinc had no significant difference in three culture models. Content of phosphorus had no significant difference between that in cage-free culture and offshore cage, was lower in the traditional cage. The cage-free cultured croakers had the highest content of calcium and phosphorus. Content of selenium was about the same between the offshore cage and the traditional cage stocking, higher in the cage-free culture. This research has considerable application value for identifying quality and sources of the big yellow croakers. 展开更多
关键词 Culture model Larimichthys crocea (big yellow croaker) Fish nutrient Quality evaluation
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