BACKGROUND Dysbacteriosis may be a crucial environmental factor for ulcerative colitis(UC).Further study is required on microbiota alterations in the gastrointestinal tract of patients with UC for better clinical mana...BACKGROUND Dysbacteriosis may be a crucial environmental factor for ulcerative colitis(UC).Further study is required on microbiota alterations in the gastrointestinal tract of patients with UC for better clinical management and treatment.AIM To analyze the relationship between different clinical features and the intestinal microbiota,including bacteria and fungi,in Chinese patients with UC.METHODS Eligible inpatients were enrolled from January 1,2018 to June 30,2019,and stool and mucosa samples were collected.UC was diagnosed by endoscopy,pathology,Mayo Score,and Montreal classification.Gene amplicon sequencing of 16S rRNA gene and fungal internal transcribed spacer gene was used to detect the intestinal microbiota composition.Alpha diversity,principal component analysis,similarity analysis,and Metastats analysis were employed to evaluate differences among groups.RESULTS A total of 89 patients with UC and 33 non-inflammatory bowel disease(IBD)controls were enrolled.For bacterial analysis,72 stool and 48 mucosa samples were obtained from patients with UC and 21 stool and 12 mucosa samples were obtained from the controls.For fungal analysis,stool samples were obtained from 43 patients with UC and 15 controls.A significant difference existed between the fecal and mucosal bacteria of patients with UC.Theα-diversity of intestinal bacteria and the relative abundance of some families,such as Lachnospiraceae and Ruminococcaceae,decreased with the increasing severity of bowel inflammation,while Escherichia-Shigella showed the opposite trend.More intermicrobial correlations in UC in remission than in active patients were observed.The bacteriafungi correlations became single and uneven in patients with UC.CONCLUSION The intestinal bacteria flora of patients with UC differs significantly in terms of various sample types and disease activities.The intermicrobial correlations change in patients with UC compared with non-IBD controls.展开更多
Celiac disease (CD),once considered a gastrointestinal condition,is now known as a systemic autoimmune disease initiated by exposure to dietary gluten mainly in human leukocyte antigen DQ2 (HLA-DQ2) or HLA-DQ8 carrier...Celiac disease (CD),once considered a gastrointestinal condition,is now known as a systemic autoimmune disease initiated by exposure to dietary gluten mainly in human leukocyte antigen DQ2 (HLA-DQ2) or HLA-DQ8 carriers.The classic presentations of CD include intestinal manifestations such as chronic diarrhea,bloating,abdominal pain,constipation,weight loss,or poor growth in children.Iron-deficiency,osteoporosis,and neuropathy attributable to vitamin B12 deficiency is also frequently seen in celiac patients.Immune responses spreading to tissues apart from the intestine cause dermatologic conditions such as dermatitis herpetiformis,and even increased risk of miscarriage in women.展开更多
基金Supported by the 13th Five-Year Plan for National Key R&D Program of China,No.2018YFC1705402National Natural Science Foundation of China,No.81770559 and No.81370500.
文摘BACKGROUND Dysbacteriosis may be a crucial environmental factor for ulcerative colitis(UC).Further study is required on microbiota alterations in the gastrointestinal tract of patients with UC for better clinical management and treatment.AIM To analyze the relationship between different clinical features and the intestinal microbiota,including bacteria and fungi,in Chinese patients with UC.METHODS Eligible inpatients were enrolled from January 1,2018 to June 30,2019,and stool and mucosa samples were collected.UC was diagnosed by endoscopy,pathology,Mayo Score,and Montreal classification.Gene amplicon sequencing of 16S rRNA gene and fungal internal transcribed spacer gene was used to detect the intestinal microbiota composition.Alpha diversity,principal component analysis,similarity analysis,and Metastats analysis were employed to evaluate differences among groups.RESULTS A total of 89 patients with UC and 33 non-inflammatory bowel disease(IBD)controls were enrolled.For bacterial analysis,72 stool and 48 mucosa samples were obtained from patients with UC and 21 stool and 12 mucosa samples were obtained from the controls.For fungal analysis,stool samples were obtained from 43 patients with UC and 15 controls.A significant difference existed between the fecal and mucosal bacteria of patients with UC.Theα-diversity of intestinal bacteria and the relative abundance of some families,such as Lachnospiraceae and Ruminococcaceae,decreased with the increasing severity of bowel inflammation,while Escherichia-Shigella showed the opposite trend.More intermicrobial correlations in UC in remission than in active patients were observed.The bacteriafungi correlations became single and uneven in patients with UC.CONCLUSION The intestinal bacteria flora of patients with UC differs significantly in terms of various sample types and disease activities.The intermicrobial correlations change in patients with UC compared with non-IBD controls.
文摘Celiac disease (CD),once considered a gastrointestinal condition,is now known as a systemic autoimmune disease initiated by exposure to dietary gluten mainly in human leukocyte antigen DQ2 (HLA-DQ2) or HLA-DQ8 carriers.The classic presentations of CD include intestinal manifestations such as chronic diarrhea,bloating,abdominal pain,constipation,weight loss,or poor growth in children.Iron-deficiency,osteoporosis,and neuropathy attributable to vitamin B12 deficiency is also frequently seen in celiac patients.Immune responses spreading to tissues apart from the intestine cause dermatologic conditions such as dermatitis herpetiformis,and even increased risk of miscarriage in women.
