Background:Stem CO_(2) efflux(E_(S))plays a critical role in the carbon budget of forest ecosystems.Thinning is a core practice for sustainable management of plantations.It is therefore necessary and urgent to study t...Background:Stem CO_(2) efflux(E_(S))plays a critical role in the carbon budget of forest ecosystems.Thinning is a core practice for sustainable management of plantations.It is therefore necessary and urgent to study the effect and mechanism of thinning intensity(TI)on E_(S).Methods:In this study,five TIs were applied in Larix principis-rupprechtii Mayr 21-,25-,and 41-year-old stands in North China in 2010.Portable infrared gas analyzer(Li-8100 A)was used to measure ES and its association with environmental factors at monthly intervals from May to October in 2013 to 2015.In addition,nutrients,wood structure and nonstructural carbon(NSC)data were measured in August 2016.Results:The results show that ES increased with increasing TI.The maximum ES values occurred at a TI of 35%(3.29,4.57 and 2.98μmol·m^(-2)·s^(-1))and were 1.54-,1.94-and 2.89-fold greater than the minimum E_(S) value in the CK stands(2.14,2.35 and 1.03μmol·m^(-2)·s^(-1))in July for the 21-,25-and 41-year-old forests,respectively.The E_(S) of the trees in low-density stands was more sensitive to temperature than that of the trees in high-density stands.Soluble sugars(SS)and temperature are the main factors affecting ES.When the stand density is low enough as 41-year-old L.principis-rupprechtii forests with TI 35%,bark thickness(BT)and humidity should be considered in addition to air temperature(T_(a)),wood temperature(T_(w)),sapwood width(SW),nitrogen concentration(N)and SS in the evaluation of ES.If a change in stand density is ignored,the CO_(2) released from individual 21-,25-and 41-year-old trees could be underestimated by 168.89%,101.94% and 200.49%,respectively.CO_(2) release was estimated based on the stem equation in combination with the factors influencing ES for reference.Conclusions:We suggest that it is not sufficient to conventional models which quantify ES only by temperature and that incorporating the associated drivers(e.g.density,SS,SW and N)based on stand density into conventional models can improve the accuracy of ES estimates.展开更多
Background:Crop-tree thinning (CTT) is a forest management practice aiming at enhancing the growth of target trees in plantations by harvesting neighboring trees. Along with the positive effect on tree growth, thinnin...Background:Crop-tree thinning (CTT) is a forest management practice aiming at enhancing the growth of target trees in plantations by harvesting neighboring trees. Along with the positive effect on tree growth, thinning represents a disturbance, which likely affects belowground organisms and may feed back to stand productivity.However, the impact of CTT on the belowground food web is poorly understood. Since nematodes are species-rich and abundant belowground organisms, occupying a variety of trophic positions in soil food webs and being sensitive to disturbances, they serve as ecological indicators of ecosystem disturbance.Results:We studied the effect of CTT on the soil nematode community structure in pine (Pinus massoniana Lamb.),Chinese fir (Cunninghamia lanceolata (Lamb.) Hook.) and cypress (Cupressus funebris Endl.) plantations in the Sichuan Basin three years after applying CTT. Crop-tree thinning significantly increased the abundance of soil nematodes in each of the plantations. Moreover, CTT significantly increased the relative abundance of herbivorous nematodes in each of the plantations. Furthermore, CTT increased the proportion of stress tolerators (c-p 1)and enrichment opportunists (c-p 2) and reduced the maturity, structure and enrichment indices of nematodes in Chinese fir and cypress plantations, while only subtle changes were observed in pine plantations. Interestingly,across plantations, the effects of CTT on soil nematode communities were mainly due to changes in microbial biomass nitrogen and understory vegetation diversity.Conclusions:Forest management practices resulting in more open canopies uniformly affect soil food webs by changing the quantity and quality of resources associated with increased understory cover and diversity as well as microbial food. These insights increase our understanding of the impacts of forest management on the structure and functioning of belowground communities, which may contribute to management and conservation policies for more sustainable forestry.展开更多
The multiple sensing provides booming options to eliminate interference and ensure the accuracy of detection by mutually coupling and validating multiple data sets.Here,we integrate the jigsaw-like multifunctional min...The multiple sensing provides booming options to eliminate interference and ensure the accuracy of detection by mutually coupling and validating multiple data sets.Here,we integrate the jigsaw-like multifunctional mini-pillar platform to perform multi-mode(electrochemical,fluorescence,surface-enhanced Raman scattering(SERS)and colorimetric)sensing in individual microdroplets.Each mini-pillar connector can parallelize together by specific concave-convex interface to form integrated jigsaw-like platform for multi-mode sensing,and each specific mini-pillar can be modified into the individual sensing unit to read the prescribed signals.We successfully implemented electrochemical,fluorescence,SERS and colorimetric detection by multiple signals coupling to reduce the false positive analysis.Such platform brings a promising clue of in-situ analysis and point-of-care testing for disease diagnosis and health monitoring.展开更多
Wearable sweat sensors with various sensing systems can provide noninvasive medical diagnostics and healthcare monitoring.Here,we demonstrate a wearable microfluidic nanoplasmonic sensor capable of refreshable and por...Wearable sweat sensors with various sensing systems can provide noninvasive medical diagnostics and healthcare monitoring.Here,we demonstrate a wearable microfluidic nanoplasmonic sensor capable of refreshable and portable recognition fingerprint information of targeted biomarkers including urea,lactate,and pH in sweat.A miniature,thin plasmonic metasurface with homogeneous mushroom-shaped hot spots and high surface-enhanced Raman scattering(SERS)activity is designed and integrated into a microfluidics platform.Compared to conventional wearable SERS platforms with the risk of mixed effect between new and old sweat,the microfluidic SERS system allows sweat administration in a controllable and high temporal-resolution fashion,providing refreshable SERS analysis.We use a portable and customized Raman analyzer with a friendly human-machine interface for portable recognition of the spectroscopic signatures of sweat biomarkers.This study integrates epidermal microfluidics with portable SERS molecular recognition,presenting a controllable,handy,and dynamical biofluid sensing system for personalized medicine.展开更多
基金funding from National Natural Science Foundation of China(No.31870387),China Scholarship Council.
文摘Background:Stem CO_(2) efflux(E_(S))plays a critical role in the carbon budget of forest ecosystems.Thinning is a core practice for sustainable management of plantations.It is therefore necessary and urgent to study the effect and mechanism of thinning intensity(TI)on E_(S).Methods:In this study,five TIs were applied in Larix principis-rupprechtii Mayr 21-,25-,and 41-year-old stands in North China in 2010.Portable infrared gas analyzer(Li-8100 A)was used to measure ES and its association with environmental factors at monthly intervals from May to October in 2013 to 2015.In addition,nutrients,wood structure and nonstructural carbon(NSC)data were measured in August 2016.Results:The results show that ES increased with increasing TI.The maximum ES values occurred at a TI of 35%(3.29,4.57 and 2.98μmol·m^(-2)·s^(-1))and were 1.54-,1.94-and 2.89-fold greater than the minimum E_(S) value in the CK stands(2.14,2.35 and 1.03μmol·m^(-2)·s^(-1))in July for the 21-,25-and 41-year-old forests,respectively.The E_(S) of the trees in low-density stands was more sensitive to temperature than that of the trees in high-density stands.Soluble sugars(SS)and temperature are the main factors affecting ES.When the stand density is low enough as 41-year-old L.principis-rupprechtii forests with TI 35%,bark thickness(BT)and humidity should be considered in addition to air temperature(T_(a)),wood temperature(T_(w)),sapwood width(SW),nitrogen concentration(N)and SS in the evaluation of ES.If a change in stand density is ignored,the CO_(2) released from individual 21-,25-and 41-year-old trees could be underestimated by 168.89%,101.94% and 200.49%,respectively.CO_(2) release was estimated based on the stem equation in combination with the factors influencing ES for reference.Conclusions:We suggest that it is not sufficient to conventional models which quantify ES only by temperature and that incorporating the associated drivers(e.g.density,SS,SW and N)based on stand density into conventional models can improve the accuracy of ES estimates.
基金supported by the National Key Research and Development Program of China (Grant No. 2017YFD060030205)the German Government loans for Sichuan Forestry Sustainable Management (Grant No. G1403083)the“Tianfu Ten Thousand Talents Plan”of Sichuan Province (Grant No. 1922999002). the financial support from the China Scholarship Council (Project No. 202006910045)
文摘Background:Crop-tree thinning (CTT) is a forest management practice aiming at enhancing the growth of target trees in plantations by harvesting neighboring trees. Along with the positive effect on tree growth, thinning represents a disturbance, which likely affects belowground organisms and may feed back to stand productivity.However, the impact of CTT on the belowground food web is poorly understood. Since nematodes are species-rich and abundant belowground organisms, occupying a variety of trophic positions in soil food webs and being sensitive to disturbances, they serve as ecological indicators of ecosystem disturbance.Results:We studied the effect of CTT on the soil nematode community structure in pine (Pinus massoniana Lamb.),Chinese fir (Cunninghamia lanceolata (Lamb.) Hook.) and cypress (Cupressus funebris Endl.) plantations in the Sichuan Basin three years after applying CTT. Crop-tree thinning significantly increased the abundance of soil nematodes in each of the plantations. Moreover, CTT significantly increased the relative abundance of herbivorous nematodes in each of the plantations. Furthermore, CTT increased the proportion of stress tolerators (c-p 1)and enrichment opportunists (c-p 2) and reduced the maturity, structure and enrichment indices of nematodes in Chinese fir and cypress plantations, while only subtle changes were observed in pine plantations. Interestingly,across plantations, the effects of CTT on soil nematode communities were mainly due to changes in microbial biomass nitrogen and understory vegetation diversity.Conclusions:Forest management practices resulting in more open canopies uniformly affect soil food webs by changing the quantity and quality of resources associated with increased understory cover and diversity as well as microbial food. These insights increase our understanding of the impacts of forest management on the structure and functioning of belowground communities, which may contribute to management and conservation policies for more sustainable forestry.
基金National Natural Science Foundation of China(Nos.21804007 and 21890742)Youth Scholars of Beijing Technology and Business University(No.QNJJ2020-04)+1 种基金SZU Top Ranking Project(No.86000000210)Shenzhen Stability Support Plan(No.20200806163622001)。
文摘The multiple sensing provides booming options to eliminate interference and ensure the accuracy of detection by mutually coupling and validating multiple data sets.Here,we integrate the jigsaw-like multifunctional mini-pillar platform to perform multi-mode(electrochemical,fluorescence,surface-enhanced Raman scattering(SERS)and colorimetric)sensing in individual microdroplets.Each mini-pillar connector can parallelize together by specific concave-convex interface to form integrated jigsaw-like platform for multi-mode sensing,and each specific mini-pillar can be modified into the individual sensing unit to read the prescribed signals.We successfully implemented electrochemical,fluorescence,SERS and colorimetric detection by multiple signals coupling to reduce the false positive analysis.Such platform brings a promising clue of in-situ analysis and point-of-care testing for disease diagnosis and health monitoring.
基金funding from Joint Fund of the Ministry of Education for Equipment Pre-research (8091B022142)Shenzhen Stability Support Plan (20200806163622001)Shenzhen Overseas Talent Program,and Shenzhen Key Laboratory for Nano Biosensing Technology (ZDSYS20210112161400001).
文摘Wearable sweat sensors with various sensing systems can provide noninvasive medical diagnostics and healthcare monitoring.Here,we demonstrate a wearable microfluidic nanoplasmonic sensor capable of refreshable and portable recognition fingerprint information of targeted biomarkers including urea,lactate,and pH in sweat.A miniature,thin plasmonic metasurface with homogeneous mushroom-shaped hot spots and high surface-enhanced Raman scattering(SERS)activity is designed and integrated into a microfluidics platform.Compared to conventional wearable SERS platforms with the risk of mixed effect between new and old sweat,the microfluidic SERS system allows sweat administration in a controllable and high temporal-resolution fashion,providing refreshable SERS analysis.We use a portable and customized Raman analyzer with a friendly human-machine interface for portable recognition of the spectroscopic signatures of sweat biomarkers.This study integrates epidermal microfluidics with portable SERS molecular recognition,presenting a controllable,handy,and dynamical biofluid sensing system for personalized medicine.