Efficient Simultaneous Detection of Metabolites Based on Electroenzymatic Assembly Strategy

Objective and Impact Statement:We describe an electroenzymatic mediator(EM)sensor based on an electroenzymatic assembly peak separation strategy,which can efficiently realize the simultaneous detection of 3 typical cardiovascular disease(CVD)metabolites in 5μl of plasma under one test.This work has substantial implications toward improving the efficiency of chronic CVD assessment.Introduction:Monitoring CVD of metabolites is strongly associated with disease risk.Independent and time-consuming detection in hospitals is unfavorable for chronic CVD management.Methods:The EM was flexibly designed by the cross-linking of electron mediators and enzymes,and 3 EM layers with different characteristics were assembled on one electrode.Electrons were transferred under tunable potential;3 metabolites were quantitatively detected by 3 peak currents that correlated with metabolite concentrations.Results:In this study,the EM sensor showed high sensitivity for the simultaneous detection of 3 metabolites with a lower limit of 0.01 mM.The linear correlation between the sensor and clinical was greater than 0.980 for 242 patients,and the consistency of risk assessment was 94.6%.Conclusion:Metabolites could be expanded by the EM,and the sensor could be a promising candidate as a home healthcare tool for CVD risk assessment.

Krüppel-like factor 8 overexpression is correlated with angiogenesis and poor prognosis in gastric cancer

AIM:To investigate Krüppel-like factor 8 (KLF8) expression in gastric cancer and its relationship with angiogenesis and prognosis of gastric cancer. METHODS:One hundred and fifty-four patients with gastric cancer who underwent successful curative resection were retrospectively enrolled in the study. Fifty tumor-adjacent healthy gastric tissues (≥ 5 cm from the tumor margin) obtained during the original resection were randomly selected for comparative analysis. In situ expression of KLF8 and CD34 proteins were examined by immunohistochemistry. The intratumoral microvessel density (MVD) was determined by manually counting the immunostained CD34-positive endothelial cells in three consecutive high-magnification fields (× 200). The relationship between differential KLF8 expression and MVD was assessed using Spearman's correlation coefficient test. χ2 test was performed to evaluate the effects of differential KLF8 expression on clinicopathologic factors. Kaplan-Meier and multivariate Cox survival analyses were used to assess the prognostic value of differential KLF8 expression in gastric cancer. RESULTS:Significantly higher levels of KLF8 protein were detected in gastric cancer tissues than in the adjacent non-cancerous tissues (54.5% vs 34.0%, P < 0.05). KLF8 expression was associated with tumor size (P < 0.001), local invasion (P = 0.005), regional lymph node metastasis (P = 0.029), distant metastasis (P = 0.023), and tumor node metastasis (TNM) stage (P = 0.002), as well as the MVD (r = 0.392, P < 0.001). Patients with KLF8 positive expression had poorer overall survival (P < 0.001) and cancer-specific survival (P < 0.001) than those with negative expression. Multivariate analysis demonstrated that KLF8 expression independently affected both overall and cancer-specific survival of gastric cancer patients (P = 0.035 and 0.042, respectively). CONCLUSION:KLF8 is closely associated with gastric tumor progression, angiogenesis and poor prognosis, suggesting it may represent a novel prognostic biomarker and therapeutic target for gastric cancer.

DNA methylation drives a new path in gastric cancer early detection:Current impact and prospects

Gastric cancer(GC)is one of the most common and deadly cancers worldwide.Early detection offers the best chance for curative treatment and reducing its mortality.However,the optimal population-based early screening for GC remains unmet.Aberrant DNA methylation occurs in the early stage of GC,exhibiting cancer-specific genetic and epigenetic changes,and can be detected in the media such as blood,gastric juice,and feces,constituting a valuable biomarker for cancer early detection.Furthermore,DNA methylation is a stable epigenetic alteration,and many innovative methods have been developed to quantify it rapidly and accurately.Nonetheless,large-scale clinical validation of DNA methylation serving as tumor biomarkers is still lacking,precluding their implementation in clinical practice.In conclusion,after a critical analysis of the recent existing literature,we summarized the evolving roles of DNA methylation during GC occurrence,expounded the newly discovered noninvasive DNA methylation biomarkers for early detection of GC,and discussed its challenges and prospects in clinical applications.

全国急诊检验现状与发展需求调研报告

目的深入调研全国二级及三级医院急诊检验现状和发展需求。方法 2021年2月1日至3月1日, 采用网络问卷调研方法, 调研全国31个省、直辖市、自治区的二级及三级医院。调研内容涉及急诊检验的场地、人员与设备配置、开展的检测项目、检测周转时间(TAT)、试剂耗材管理、分析前质量管理、信息化管理、危急值管理、生物安全等各个方面。结果本次调研共收集到2 187份调研问卷, 剔除部分不合格问卷, 得到有效问卷1 503份, 其中二级医院755份, 三级医院748份。29.41%(220/748)的三级医院日均急诊患者量超过300人次, 而76.69%(579/755)的二级医院日均急诊患者量不足100人次;24.47%(183/748)的三级医院日均急诊检验测试量超过5 000个, 93.51%(706/755)的二级医院日均急诊测试量不足2 000个;在设置有独立急诊检验单元的医院中, 68.79%(238/346)的三级医院急诊检验单元面积在100 m2以下;总计56.02%(842/1 503)的医院急诊检验无固定工作人员;8.65%(130/1 503)的医院急诊检验生化免疫设备无加急通道;8.38%(126/1 503)的医院急诊检验无试剂耗材出入库记录;24.62%(370/1 503)的医院急诊检验未定期进行不合格标本统计工作;58.62%(881/1 503)的医院急诊检验未设置急诊检验标本的优先级;48.64%(731/1 503)的医院急诊检验没有监测标本全流程的功能;7.32%(110/1 503)的医院急诊检验未就危急值相关问题与临床进行沟通;50.23%(755/1 503)的急诊检验参研者表示目前实验室不存在生物安全风险。结论调研较客观地呈现了我国急诊检验现状和发展需求, 也反映出在临床沟通、场地和人员配置、急诊标本分级、试剂耗材管理、信息化建设、生物安全管理等方面尚有很多需重点改进的流程或理念, 应得到管理者较广泛的关注。