Sweet cherry(Prunus avium)is a popular fruit with high nutritional value and excellent flavor.Although pollen plays an important role in the double fertilization and subsequent fruit production of this species,little ...Sweet cherry(Prunus avium)is a popular fruit with high nutritional value and excellent flavor.Although pollen plays an important role in the double fertilization and subsequent fruit production of this species,little is known about its pollen tube transcriptome.In this study,we identified 16,409 transcripts using single-molecule sequencing.After filtering 292 transposable elements,we conducted further analyses including mRNA classification,gene function prediction,alternative splicing(AS)analysis,and long noncoding RNA(lncRNA)identification to gain insight into the pollen transcriptome.The filtered transcripts could be matched with 3,438 coding region sequences from the sweet cherry genome.GO and KEGG analyses revealed complex biological processes during pollen tube elongation.A total of 2043 AS events were predicted,7 of which were identified in different organs,such as the leaf,pistil and pollen tube.Using BLASTnt and the Coding-Potential Assessment Tool(CPAT),we distinguished a total of 284 lncRNAs,among which 154 qualified as natural antisense transcripts(NATs).As the NATs could be the reverse complements of coding mRNA sequences,they might bind to coding sequences.Antisense transfection assays showed that the NATs could regulate the expression levels of their complementary sequences and even affect the growth conditions of pollen tubes.In summary,this research characterizes the transcripts of P.avium pollen and lays the foundation for elucidating the physiological and biochemical mechanisms underlying sexual reproduction in the male gametes of this species.展开更多
Sweet cherry(Prunus avium L.)is an economic fruit tree of the Prunus genus in the Roaceae family(Vignati et al.2022).It is popular with consumers because of its early ripening in open-field,bright color,and rich nutri...Sweet cherry(Prunus avium L.)is an economic fruit tree of the Prunus genus in the Roaceae family(Vignati et al.2022).It is popular with consumers because of its early ripening in open-field,bright color,and rich nutrition.Since the 80’s,sweet cherry industry in our country has developed rapidly.At present,the production,planting area and import of sweet cherry are ranked first in the world,which is known as‘gold planting industry’(Zhang et al.2020).展开更多
基金supported by the National Natural Science Foundation of China(31272123)National Science Foundation for Young Scientists of China(31601726).
文摘Sweet cherry(Prunus avium)is a popular fruit with high nutritional value and excellent flavor.Although pollen plays an important role in the double fertilization and subsequent fruit production of this species,little is known about its pollen tube transcriptome.In this study,we identified 16,409 transcripts using single-molecule sequencing.After filtering 292 transposable elements,we conducted further analyses including mRNA classification,gene function prediction,alternative splicing(AS)analysis,and long noncoding RNA(lncRNA)identification to gain insight into the pollen transcriptome.The filtered transcripts could be matched with 3,438 coding region sequences from the sweet cherry genome.GO and KEGG analyses revealed complex biological processes during pollen tube elongation.A total of 2043 AS events were predicted,7 of which were identified in different organs,such as the leaf,pistil and pollen tube.Using BLASTnt and the Coding-Potential Assessment Tool(CPAT),we distinguished a total of 284 lncRNAs,among which 154 qualified as natural antisense transcripts(NATs).As the NATs could be the reverse complements of coding mRNA sequences,they might bind to coding sequences.Antisense transfection assays showed that the NATs could regulate the expression levels of their complementary sequences and even affect the growth conditions of pollen tubes.In summary,this research characterizes the transcripts of P.avium pollen and lays the foundation for elucidating the physiological and biochemical mechanisms underlying sexual reproduction in the male gametes of this species.
基金Open access funding provided by Shanghai Jiao Tong Universitysupported by Beijing New-star Plan of Science and Technology(2010B025)+1 种基金the Promotion and Innovation of Beijing Academy of Agriculture and Forestry Sciences(KJCX201910)the technological innovation ability project of Beijing Academy of Agriculture and Forestry Sciences(KJCX20210403).
文摘Sweet cherry(Prunus avium L.)is an economic fruit tree of the Prunus genus in the Roaceae family(Vignati et al.2022).It is popular with consumers because of its early ripening in open-field,bright color,and rich nutrition.Since the 80’s,sweet cherry industry in our country has developed rapidly.At present,the production,planting area and import of sweet cherry are ranked first in the world,which is known as‘gold planting industry’(Zhang et al.2020).
