1.Introduction Noble metal nanocrystals serve as exceptional catalysts across a wide spectrum of industrial processes[1].Moreover,there exists a substantial demand for noble metal catalysts in emerging clean-energy ap...1.Introduction Noble metal nanocrystals serve as exceptional catalysts across a wide spectrum of industrial processes[1].Moreover,there exists a substantial demand for noble metal catalysts in emerging clean-energy applications,such as water-splitting hydrogen production and fuel cells.Incorporating non-noble transition metals or main-group metals into noble metal nanocrystals can reduce the weight of noble metals in the catalysts,thus remarkably lowering the cost.Furthermore,alloy nanocrystals offer intriguing catalytic properties that surpass those of their monometallic counterparts,thanks to the charge transfer between the constituent components and the expansion or shrinkage of the lattice size[2].展开更多
Sub-100 nm hollow carbon nanospheres with thin shells are highly desirable anode materials for energy storage applications. However, their synthesis remains a great challenge with conventional strategies. In this work...Sub-100 nm hollow carbon nanospheres with thin shells are highly desirable anode materials for energy storage applications. However, their synthesis remains a great challenge with conventional strategies. In this work, we demonstrate that hollow carbon nanospheres of unprecedentedly small sizes (down to - 32.5 nm and with thickness of - 3.9 nm) can be produced on a large scale by a templating process in a unique reverse micelle system. Reverse micelles enable a spatially confined Stober process that produces uniform silica nanospheres with significantly reduced sizes compared with those from a conventional Stober process, and a subsequent well-controlled sol-gel coating process with a resorcinol-formaldehyde resin on these silica nanospheres as a precursor of the hollow carbon nanospheres. Owing to the short diffusion length resulting from their hollow structure, as well as their small size and microporosity, these hollow carbon nanospheres show excellent capacity and cycling stability when used as anode materials for lithium/sodium-ion batteries.展开更多
Regulation of osteogenic differentiation of bone mesenchymal stromal cells (BMSCs) plays a critical role in bone regeneration. As small non-coding RNAs, microRNAs (miRNAs) play an important role in stem cell diffe...Regulation of osteogenic differentiation of bone mesenchymal stromal cells (BMSCs) plays a critical role in bone regeneration. As small non-coding RNAs, microRNAs (miRNAs) play an important role in stem cell differentiation through regulating target-mRNA expression. Unfortunately, highly efficient and safe delivery of miRNAs to BMSCs to regulate their osteogenic differentiation remains challenging. Conventional inorganic nanocrystals have shown increased toxicity owing to their larger size precluding renal clearance. Here, we developed novel, surface-engineered, ultra-small gold nanoparticles (USAuNPs, 〈10 nm) for use as highly efficient miR-5106-delivery systems to enable regulation of BMSC differentiation. We exploited the effects of AuNPs coated layer-by-layer with polyethylenimine (PEI) and liposomes (Lipo) to enhance miR-5106-delivery activity and subsequent BMSC differentiation capacity. The PEI- and Lipo-coated AuNPs (Au@PEI@Lipo) showed negligible cytotoxicity, good miRNA-5106-binding affinity, highly efficient delivery of miRNAs to BMSCs, and long-term miRNA expression (21 days). Additionally, compared with commercial Lipofectamine 3000 and 25 kD PEI, the optimized Au@PEI@Lipo-miR-5106 nanocomplexes significantly enhanced BMSC differentiation into osteoblast-like cells through activation of the Sox9 transcription factor. Our findings reveal a promising strategy for the rational design of ultra-small inorganic nanoparticles as highly efficient miRNA-delivery platforms for tissue regeneration and disease therapy.展开更多
It is highly desirable,while still challenging,to obtain noble metal nanocrystals with custom capping ligands,because their colloidal synthesis relies on specific capping ligands for the shape control while convention...It is highly desirable,while still challenging,to obtain noble metal nanocrystals with custom capping ligands,because their colloidal synthesis relies on specific capping ligands for the shape control while conventional ligand exchange processes suffer from“the strong replaces the weak”limitation,which greatly hinders their applications.Herein,we report a general and effective ligand exchange approach that can replace the native capping ligands of noble metal nanocrystals with virtually any type of ligands,producing flexibly tailored surface properties.The key is to use diethylamine with conveniently switchable binding affinity to the metal surface as an intermediate ligand.As a strong ligand,it in its original form can effectively remove the native ligands;while protonated,it loses its binding affinity and facilitates the adsorption of new ligands,especially weak ones,onto the metal surface.By this means,the irreversible order in the conventional ligand exchange processes could be overcome.The efficacy of the strategy is demonstrated by mutual exchange of the capping ligands among cetyltrimethylammonium,citrate,polyvinylpyrrolidone,and oleylamine.This novel strategy significantly expands our ability to manipulate the surface property of noble metal nanocrystals and extends their applicability to a wide range of fields,particularly biomedical applications.展开更多
基金support from the China Postdoctoral Science Foundation(2023TQ0263)(Z.L.)National Natural Science Foundation of China(22071191)(C.G.)+1 种基金the Key Research and Development Program of Shaanxi Province(2021GXLH-Z-022)(C.G.)the Fundamental Research Funds for the Central Universities.
文摘1.Introduction Noble metal nanocrystals serve as exceptional catalysts across a wide spectrum of industrial processes[1].Moreover,there exists a substantial demand for noble metal catalysts in emerging clean-energy applications,such as water-splitting hydrogen production and fuel cells.Incorporating non-noble transition metals or main-group metals into noble metal nanocrystals can reduce the weight of noble metals in the catalysts,thus remarkably lowering the cost.Furthermore,alloy nanocrystals offer intriguing catalytic properties that surpass those of their monometallic counterparts,thanks to the charge transfer between the constituent components and the expansion or shrinkage of the lattice size[2].
基金C. B. G. acknowledges the support from the National Natural Science Foundation of China (Nos. 21671156 and 21301138), the Tang Scholar Program from the Cyrus Tang Foundation, and the start-up fund from Xi'an Jiaotong University. X. G. H acknowledges the programs supported by State Key Laboratory of Electrical Insulation and Power Equipment (No. EIPE17306) and Young Talent Support Plan of Xi'an Jiaotong University. Y. D. Y. acknowledges the support from U.S. Department of Energy (No. DE-SC0002247).
文摘Sub-100 nm hollow carbon nanospheres with thin shells are highly desirable anode materials for energy storage applications. However, their synthesis remains a great challenge with conventional strategies. In this work, we demonstrate that hollow carbon nanospheres of unprecedentedly small sizes (down to - 32.5 nm and with thickness of - 3.9 nm) can be produced on a large scale by a templating process in a unique reverse micelle system. Reverse micelles enable a spatially confined Stober process that produces uniform silica nanospheres with significantly reduced sizes compared with those from a conventional Stober process, and a subsequent well-controlled sol-gel coating process with a resorcinol-formaldehyde resin on these silica nanospheres as a precursor of the hollow carbon nanospheres. Owing to the short diffusion length resulting from their hollow structure, as well as their small size and microporosity, these hollow carbon nanospheres show excellent capacity and cycling stability when used as anode materials for lithium/sodium-ion batteries.
基金Acknowledgements We acknowledge the valuable comments of potential reviewers. This work was supported by State Key Laboratory for Mechanical Behavior of Materials, the Scientific Research Starting Foundation from Xi'an Jiaotong University (No. DW011798N3000010), the Fundamental Research Funds for the Central Universities (No. XJJ2014090), the Natural Science Basic Research Plan in Shaanxi Province of China (No. 2015JQ5165), and National Natural Science Foundation of China (No. 51502237).
文摘Regulation of osteogenic differentiation of bone mesenchymal stromal cells (BMSCs) plays a critical role in bone regeneration. As small non-coding RNAs, microRNAs (miRNAs) play an important role in stem cell differentiation through regulating target-mRNA expression. Unfortunately, highly efficient and safe delivery of miRNAs to BMSCs to regulate their osteogenic differentiation remains challenging. Conventional inorganic nanocrystals have shown increased toxicity owing to their larger size precluding renal clearance. Here, we developed novel, surface-engineered, ultra-small gold nanoparticles (USAuNPs, 〈10 nm) for use as highly efficient miR-5106-delivery systems to enable regulation of BMSC differentiation. We exploited the effects of AuNPs coated layer-by-layer with polyethylenimine (PEI) and liposomes (Lipo) to enhance miR-5106-delivery activity and subsequent BMSC differentiation capacity. The PEI- and Lipo-coated AuNPs (Au@PEI@Lipo) showed negligible cytotoxicity, good miRNA-5106-binding affinity, highly efficient delivery of miRNAs to BMSCs, and long-term miRNA expression (21 days). Additionally, compared with commercial Lipofectamine 3000 and 25 kD PEI, the optimized Au@PEI@Lipo-miR-5106 nanocomplexes significantly enhanced BMSC differentiation into osteoblast-like cells through activation of the Sox9 transcription factor. Our findings reveal a promising strategy for the rational design of ultra-small inorganic nanoparticles as highly efficient miRNA-delivery platforms for tissue regeneration and disease therapy.
基金C.G.acknowledges the support from the National Natural Science Foundation of China(21671156)the Fundamental Research Funds for the Central Universities+3 种基金the World-Class Universities(Disciplines)and the Characteristic Development Guidance Funds for the Central Universities,and the Tang Scholar Program from Cyrus Tang FoundationT.C.acknowledges the support from the Collaborative Innovation Center of Suzhou Nano Science and Technology,the Priority Academic Program Development of Jiangsu Higher Education Institutions(PAPD)the 111 ProjectY.Y.acknowledges the support from the U.S.National Science Foundation(CHE-1308587).
文摘It is highly desirable,while still challenging,to obtain noble metal nanocrystals with custom capping ligands,because their colloidal synthesis relies on specific capping ligands for the shape control while conventional ligand exchange processes suffer from“the strong replaces the weak”limitation,which greatly hinders their applications.Herein,we report a general and effective ligand exchange approach that can replace the native capping ligands of noble metal nanocrystals with virtually any type of ligands,producing flexibly tailored surface properties.The key is to use diethylamine with conveniently switchable binding affinity to the metal surface as an intermediate ligand.As a strong ligand,it in its original form can effectively remove the native ligands;while protonated,it loses its binding affinity and facilitates the adsorption of new ligands,especially weak ones,onto the metal surface.By this means,the irreversible order in the conventional ligand exchange processes could be overcome.The efficacy of the strategy is demonstrated by mutual exchange of the capping ligands among cetyltrimethylammonium,citrate,polyvinylpyrrolidone,and oleylamine.This novel strategy significantly expands our ability to manipulate the surface property of noble metal nanocrystals and extends their applicability to a wide range of fields,particularly biomedical applications.