Due to sexual dimorphism in the growth of certain cultured fish species,the production of monosex fishes is desirable for the aquaculture industry.Nowadays,the most widely practiced technique available for the mass pr...Due to sexual dimorphism in the growth of certain cultured fish species,the production of monosex fishes is desirable for the aquaculture industry.Nowadays,the most widely practiced technique available for the mass production of monosex fish populations is sex steroid-induced sex reversal.Here,a novel strategy for the successful production of all-female(AF)common carp(Cyprinus carpio L.),to take advantage of the sexual dimorphism in growth documented in this species,has been developed using genetic engineering via single gene-targeting manipulation without any exogenous hormone treatments.Male and female heterozygous cyp17a1-deficient common carp were first obtained using the clustered regularly interspaced short palindromic repeats/CRISPR-associated endonuclease 9(CRISPR/Cas9)technique.An all-male phenotype for homozygous cyp17a1-deficient carp,regardless of the individuals’sexdetermination genotypes(XY or XX),has been observed.A male-specific DNA marker newly identified in our laboratory was used to screen the neomale carp population with the XX genotype from the cyp17a1-deficient carp.These neomale carp develop a normal testis structure with normal spermatogenesis and sperm capacity.The neomale common carp were then mated with wild-type(WT)females(cyp17a1^(+/+)XX genotype)using artificial fertilization.All the AF offspring sample fish from the neomale-WT female mating were confirmed as having the cyp17a^(1+/-)XX genotype,and normal development of gonads to ovaries was observed in 100.00%of this group at eight months post-fertilization(mpf).A total of 1000 carp fingerlings,500 from the WT male and female and 500 from the neomale and WT female mating,were mixed and reared in the same pond.The average body weight of cyp17a1^(+/-)XX females was higher by 6.60%(8 mpf)and 32.66%(12 mpf)than that of the control common carp.Our study demonstrates the first successful production of a monosex teleost population with the advantages of sexual dimorphism in growth using genetic manipulation targeting a single locus.展开更多
Deployable space structure technology is an approach used in building spacecraft,especially when realizing deployment and folding functions.Once in orbit,the structures are released from the fairing,deployed,and posit...Deployable space structure technology is an approach used in building spacecraft,especially when realizing deployment and folding functions.Once in orbit,the structures are released from the fairing,deployed,and positioned.With the development of communication,remote-sensing,and navigation satellites,space-deployable structures have become cutting-edge research topics in space science and technology.This paper summarizes the current research status and development trend of spacedeployable structures in China,including large space mesh antennas,space solar arrays,and deployable structures and mechanisms for deep-space exploration.Critical technologies of space-deployable structures are addressed from the perspectives of deployable mechanisms,cable-membrane form-finding,dynamic analysis,reliable environmental adaptability analysis,and validation.Finally,future technology developments and trends are elucidated in the fields of mesh antennas,solar arrays,deployable mechanisms,and on-orbit adjustment,assembly,and construction.展开更多
With the development of various navigation systems(such as GLONASS,Galileo,BDS),there is a sharp increase in the number of visible satellites.Accordingly,the probability of multiply gross measurements will increase.Ho...With the development of various navigation systems(such as GLONASS,Galileo,BDS),there is a sharp increase in the number of visible satellites.Accordingly,the probability of multiply gross measurements will increase.However,the conventional RAIM methods are difficult to meet the demands of the navigation system.In order to solve the problem of checking and identify multiple gross errors of receiver autonomous integrity monitoring(RAIM),this paper designed full matrix of single point positioning by QR decomposition,and proposed a new RAIM algorithm based on fuzzy clustering analysis with fuzzy c-means(FCM).And on the condition of single or two gross errors,the performance of hard or fuzzy clustering analysis were compared.As the results of the experiments,the fuzzy clustering method based on FCM principle could detect multiple gross error effectively,also achieved the quality control of single point positioning and ensured better reliability results.展开更多
To meet the demands for the data combination with multiple space geodetic techniques at the observation level,we developed a new software platform with high extensibility and computation efficiency,named space Geodeti...To meet the demands for the data combination with multiple space geodetic techniques at the observation level,we developed a new software platform with high extensibility and computation efficiency,named space Geodetic SpatioTemporal data Analysis and Research software(GSTAR).Most of the modules in the GSTAR are coded in C++with object-oriented programming.The layered modular theory is adopted for the design of the software,and the antenna-based data architecture is proposed for users to construct personalized geodetic application scenarios easily.The initial performance of the GSTAR software is evaluated by processing the Global Navigation Satellite System(GNSS)data collected from 315 globally distributed stations over two and a half years.The accuracy of GNSS-based geodetic products is evaluated by comparing them with those released by International GNSS Service(IGS)Analysis Centers(AC).Taking the products released by European Space Agency(ESA)as reference,the Three-Dimension(3D)Root-Mean-Squares(RMS)of the orbit differences are 2.7/6.7/3.3/7.7/21.0 cm and the STandard Deviations(STD)of the clock differences are 19/48/16/32/25 ps for Global Positioning System(GPS),GLObal NAvigation Satellite System(GLONASS),Galileo navigation satellite system(Galileo),BeiDou Navigation Satellite System(BDS),Medium Earth Orbit(MEO),and BDS Inclined Geo-Synchronous Orbit(IGSO)satellites,respectively.The mean values of the X and Y components of the polar coordinate and the Length of Day(LOD)with respect to the International Earth Rotation and Reference Systems Service(IERS)14 C04 products are-17.6 microarc-second(μas),9.2μas,and 14.0μs/d.Compared to the IGS daily solution,the RMSs of the site position differences in the north/east/up direction are 1.6/1.5/3.9,3.8/2.4/7.6,2.5/2.4/7.9 and 2.7/2.3/7.4 mm for GPS-only,GLONASS-only,Galileo-only,and BDS-only solution,respectively.The RMSs of the differences of the tropospheric Zenith Path Delay(ZPD),the north gradients,and the east gradients are 5.8,0.9,and 0.9 mm with respect to the IGS products.The X and Y components of the geocenter motion estimated from GPS-only,Galileo-only,and BDS-only observations well agree with IGS products,while the Z component values are much nosier where anomalous harmonics in GNSS draconitic year can be found.The accuracies of the above products calculated by the GSTAR are comparable with those from different IGS ACs.Compared to the precise scientific orbit products,the 3D RMS of the orbit differences for the two Gravity Recovery and Climate Experiment Follow-on(GRACE-FO)satellites is below 1.5 cm by conducting Precise Point Positioning with Ambiguity Resolution(PPP-AR).In addition,a series of rapid data processing algorithms are developed,and the operation speed of the GSTAR software is 5.6 times faster than that of the Positioning and Navigation Data Analyst(PANDA)software for the quad-system precise orbit determination procedure.展开更多
The Least Squares Residual(LSR)algorithm,one of the classical Receiver Autonomous Integrity Monitoring(RAIM)algorithms for Global Navigation Satellite System(GNSS),presents a high Missed Detection Risk(MDR)for a large...The Least Squares Residual(LSR)algorithm,one of the classical Receiver Autonomous Integrity Monitoring(RAIM)algorithms for Global Navigation Satellite System(GNSS),presents a high Missed Detection Risk(MDR)for a large-slope faulty satellite and a high False Alarm Risk(FAR)for a small-slope faulty satellite.From the theoretical analysis of the high MDR and FAR cause,the optimal slope is determined,and thereby the optimal test statistic for fault detection is conceived,which can minimize the FAR with the MDR not exceeding its allowable value.To construct a test statistic approximate to the optimal one,the CorrelationWeighted LSR(CW-LSR)algorithm is proposed.The CW-LSR test statistic remains the sum of pseudorange residual squares,but the square for the most potentially faulty satellite,judged by correlation analysis between the pseudorange residual and observation error,is weighted with an optimal-slope-based factor.It does not obey the same distribution but has the same noncentral parameter with the optimal test statistic.The superior performance of the CW-LSR algorithm is verified via simulation,both reducing the FAR for a small-slope faulty satellite with the MDR not exceeding its allowable value and reducing the MDR for a large-slope faulty satellite at the expense of FAR addition.展开更多
The endocrine regulatory roles of the hypothalamic-pituitary-adrenocortical axis on anxiety-like behavior and metabolic status have been found throughout animal taxa.However,the precise effects of the balancing adrena...The endocrine regulatory roles of the hypothalamic-pituitary-adrenocortical axis on anxiety-like behavior and metabolic status have been found throughout animal taxa.However,the precise effects of the balancing adrenal corticosteroid biosynthesis under the influence of adrenocorticotrophic hormone(ACTH),a pro-opiomelanocortin(POMC)-derived peptide,on animal energy expenditure and somatic growth remain unknown.POMC has also been identified as one of the candidate loci for polycystic ovary syndrome,which features hyperandrogenism and some prevalence of obesity in patients.Here we show that zebrafish lacking functional POMCa exhibit similar phenotypes of stress response and body weight gain but not obesity as observed in mammalian models.In contrast with the impaired anorexigenic signaling cascade of melanocyte-stimulating hormones and leptin,which are responsible for their obesity-prone weight gain observed in various pome mutant mammals,analyses with our pomca mutant series indicate that ACTH is the key regulator for the phenotype with enhanced somatic growth without obesity in pomca-deficient zebrafish.Hypocortisolism associated with hyperandrogenism has been observed in the pomca-deficient zebrafish,with enhanced activation of mammalian target of rapamycin complex 1;reutilization of amino acids and fatty acid^-oxidation are observed in the muscle tissue of the pomca-deficient fish.After reducing hyperandrogenism by crossing our pomca mutant fish with a cy p l 7a 1-deficient background,the phenotype of enhanced somatic growth in pomca-deficient fish was no longer observed.Thus,our work also demonstrated that the role of POMCa in stress response seems to be conserved in vertebrates,whereas its effect on adipostasis is unique to teleosts.展开更多
Improvement in fish feed conversion efficiency(FCE)is beneficial for sustaining global food fish supplies.Here,we show that a set of polymorphisms at locus of the corticotropin releasing hormone receptor 2(crhr2),whic...Improvement in fish feed conversion efficiency(FCE)is beneficial for sustaining global food fish supplies.Here,we show that a set of polymorphisms at locus of the corticotropin releasing hormone receptor 2(crhr2),which is involved in hypothalamuspituitary-interrenal(HPI)axis signaling,is associated with improved FCE in farmed allogynogenetic gibel carp strain CAS Ⅲ compared with that in the wild gibel carp strain Dongting(DT).This set of polymorphisms downregulates the expression levels of crhr2 mRNA in the brain and pituitary tissues in gibel carp strain CAS Ⅲ compared with those in strain DT.Furthermore,compromised HPI axis signaling is observed in gibel carp strain CAS Ⅲ,such as decreased α-melanocyte stimulating hormone protein levels,plasma cortisol content,and stress responses.Moreover,enhanced activation of protein kinase B/mammalian target of rapamycin complex 1 signaling observed in the muscle tissue of strain CAS Ⅲ in comparison to that in strain DT indicated elevated anabolic metabolism in strain CAS Ⅲ.Thus,these studies demonstrate that the genetic markers associated with compromised HPI axis signaling,such as crhr2,are potentially useful for genetic selection toward improvement in farmed fish growth and FCE,which would reduce fishmeal consumption and thereby indirectly facilitate sustainable fisheries.展开更多
The International GNSS Service(IGS) has been providing reliable Global Ionospheric Maps(GIMs) since 1998. The Ionosphere Associate Analysis Centers(IAACs) model the global ionospheric Total Electron Content(TEC) and g...The International GNSS Service(IGS) has been providing reliable Global Ionospheric Maps(GIMs) since 1998. The Ionosphere Associate Analysis Centers(IAACs) model the global ionospheric Total Electron Content(TEC) and generate the daily GIM products within the context of the IGS. However, the rapid and final daily GIM products have a latency of at least one day and one week or so, respectively. This limits the value of GIM products in real-time GNSS applications.We propose and develop an approach for near real-time modeling of global ionospheric TEC by using the hourly IGS data. We perform an experiment in a real operating environment to generate near real-time GIM(named BUHG) products for more than two years. Final daily GIM products,Precise Point Positioning(PPP) based VTEC resources, and JASON-3 Vertical TEC(VTEC) measurements are collected for testing the performance of BUHG. The results show that the performance of BUHG is very close to that of the daily GIM products. Also, there is good agreement between BUHG and PPP-derived VTEC as well as with JASON-3 VTEC. It is possible that BUHG would be further improved with an increase in available hourly GNSS data.展开更多
基金the National Key Research and Development Program,China(2018YFD0900205)to Zhan Yinthe National Natural Science Foundation,China(31972779 and 31530077)to Gang Zhai and Zhan Yin+2 种基金the Pilot Program A Project from the Chinese Academy of Sciences(XDA24010206)to Zhan Yinthe Youth Innovation Promotion Association of CAS(20200336)to Gang Zhaithe State Key Laboratory of Freshwater Ecology and Biotechnology(2016FBZ05)to Zhan Yin.
文摘Due to sexual dimorphism in the growth of certain cultured fish species,the production of monosex fishes is desirable for the aquaculture industry.Nowadays,the most widely practiced technique available for the mass production of monosex fish populations is sex steroid-induced sex reversal.Here,a novel strategy for the successful production of all-female(AF)common carp(Cyprinus carpio L.),to take advantage of the sexual dimorphism in growth documented in this species,has been developed using genetic engineering via single gene-targeting manipulation without any exogenous hormone treatments.Male and female heterozygous cyp17a1-deficient common carp were first obtained using the clustered regularly interspaced short palindromic repeats/CRISPR-associated endonuclease 9(CRISPR/Cas9)technique.An all-male phenotype for homozygous cyp17a1-deficient carp,regardless of the individuals’sexdetermination genotypes(XY or XX),has been observed.A male-specific DNA marker newly identified in our laboratory was used to screen the neomale carp population with the XX genotype from the cyp17a1-deficient carp.These neomale carp develop a normal testis structure with normal spermatogenesis and sperm capacity.The neomale common carp were then mated with wild-type(WT)females(cyp17a1^(+/+)XX genotype)using artificial fertilization.All the AF offspring sample fish from the neomale-WT female mating were confirmed as having the cyp17a^(1+/-)XX genotype,and normal development of gonads to ovaries was observed in 100.00%of this group at eight months post-fertilization(mpf).A total of 1000 carp fingerlings,500 from the WT male and female and 500 from the neomale and WT female mating,were mixed and reared in the same pond.The average body weight of cyp17a1^(+/-)XX females was higher by 6.60%(8 mpf)and 32.66%(12 mpf)than that of the control common carp.Our study demonstrates the first successful production of a monosex teleost population with the advantages of sexual dimorphism in growth using genetic manipulation targeting a single locus.
基金financial support from the National Natural Science Foundation of China(11290154 and U20B2033)。
文摘Deployable space structure technology is an approach used in building spacecraft,especially when realizing deployment and folding functions.Once in orbit,the structures are released from the fairing,deployed,and positioned.With the development of communication,remote-sensing,and navigation satellites,space-deployable structures have become cutting-edge research topics in space science and technology.This paper summarizes the current research status and development trend of spacedeployable structures in China,including large space mesh antennas,space solar arrays,and deployable structures and mechanisms for deep-space exploration.Critical technologies of space-deployable structures are addressed from the perspectives of deployable mechanisms,cable-membrane form-finding,dynamic analysis,reliable environmental adaptability analysis,and validation.Finally,future technology developments and trends are elucidated in the fields of mesh antennas,solar arrays,deployable mechanisms,and on-orbit adjustment,assembly,and construction.
文摘With the development of various navigation systems(such as GLONASS,Galileo,BDS),there is a sharp increase in the number of visible satellites.Accordingly,the probability of multiply gross measurements will increase.However,the conventional RAIM methods are difficult to meet the demands of the navigation system.In order to solve the problem of checking and identify multiple gross errors of receiver autonomous integrity monitoring(RAIM),this paper designed full matrix of single point positioning by QR decomposition,and proposed a new RAIM algorithm based on fuzzy clustering analysis with fuzzy c-means(FCM).And on the condition of single or two gross errors,the performance of hard or fuzzy clustering analysis were compared.As the results of the experiments,the fuzzy clustering method based on FCM principle could detect multiple gross error effectively,also achieved the quality control of single point positioning and ensured better reliability results.
基金This work was sponsored by National Natural Science Foundation of China(Grant No.41931075,42274041).
文摘To meet the demands for the data combination with multiple space geodetic techniques at the observation level,we developed a new software platform with high extensibility and computation efficiency,named space Geodetic SpatioTemporal data Analysis and Research software(GSTAR).Most of the modules in the GSTAR are coded in C++with object-oriented programming.The layered modular theory is adopted for the design of the software,and the antenna-based data architecture is proposed for users to construct personalized geodetic application scenarios easily.The initial performance of the GSTAR software is evaluated by processing the Global Navigation Satellite System(GNSS)data collected from 315 globally distributed stations over two and a half years.The accuracy of GNSS-based geodetic products is evaluated by comparing them with those released by International GNSS Service(IGS)Analysis Centers(AC).Taking the products released by European Space Agency(ESA)as reference,the Three-Dimension(3D)Root-Mean-Squares(RMS)of the orbit differences are 2.7/6.7/3.3/7.7/21.0 cm and the STandard Deviations(STD)of the clock differences are 19/48/16/32/25 ps for Global Positioning System(GPS),GLObal NAvigation Satellite System(GLONASS),Galileo navigation satellite system(Galileo),BeiDou Navigation Satellite System(BDS),Medium Earth Orbit(MEO),and BDS Inclined Geo-Synchronous Orbit(IGSO)satellites,respectively.The mean values of the X and Y components of the polar coordinate and the Length of Day(LOD)with respect to the International Earth Rotation and Reference Systems Service(IERS)14 C04 products are-17.6 microarc-second(μas),9.2μas,and 14.0μs/d.Compared to the IGS daily solution,the RMSs of the site position differences in the north/east/up direction are 1.6/1.5/3.9,3.8/2.4/7.6,2.5/2.4/7.9 and 2.7/2.3/7.4 mm for GPS-only,GLONASS-only,Galileo-only,and BDS-only solution,respectively.The RMSs of the differences of the tropospheric Zenith Path Delay(ZPD),the north gradients,and the east gradients are 5.8,0.9,and 0.9 mm with respect to the IGS products.The X and Y components of the geocenter motion estimated from GPS-only,Galileo-only,and BDS-only observations well agree with IGS products,while the Z component values are much nosier where anomalous harmonics in GNSS draconitic year can be found.The accuracies of the above products calculated by the GSTAR are comparable with those from different IGS ACs.Compared to the precise scientific orbit products,the 3D RMS of the orbit differences for the two Gravity Recovery and Climate Experiment Follow-on(GRACE-FO)satellites is below 1.5 cm by conducting Precise Point Positioning with Ambiguity Resolution(PPP-AR).In addition,a series of rapid data processing algorithms are developed,and the operation speed of the GSTAR software is 5.6 times faster than that of the Positioning and Navigation Data Analyst(PANDA)software for the quad-system precise orbit determination procedure.
基金co-supported by the National Natural Science Foundation of China (Nos. 41804024, 41804026)the Open Fund of Shaanxi Key Laboratory of Integrated and Intelligent Navigation of China (No. SKLIIN-20190205)
文摘The Least Squares Residual(LSR)algorithm,one of the classical Receiver Autonomous Integrity Monitoring(RAIM)algorithms for Global Navigation Satellite System(GNSS),presents a high Missed Detection Risk(MDR)for a large-slope faulty satellite and a high False Alarm Risk(FAR)for a small-slope faulty satellite.From the theoretical analysis of the high MDR and FAR cause,the optimal slope is determined,and thereby the optimal test statistic for fault detection is conceived,which can minimize the FAR with the MDR not exceeding its allowable value.To construct a test statistic approximate to the optimal one,the CorrelationWeighted LSR(CW-LSR)algorithm is proposed.The CW-LSR test statistic remains the sum of pseudorange residual squares,but the square for the most potentially faulty satellite,judged by correlation analysis between the pseudorange residual and observation error,is weighted with an optimal-slope-based factor.It does not obey the same distribution but has the same noncentral parameter with the optimal test statistic.The superior performance of the CW-LSR algorithm is verified via simulation,both reducing the FAR for a small-slope faulty satellite with the MDR not exceeding its allowable value and reducing the MDR for a large-slope faulty satellite at the expense of FAR addition.
基金This work was supported by the National Key R&D Program of China(2018YFD0900404 to J.He and 2018YFD0900205 to Z.Y.)the National Natural Science Foundation of China(31530077 to Z.Y.)the Pilot Program A Project from the Chinese Academy of Sciences (XDA08010405 to Z.Y.).
文摘The endocrine regulatory roles of the hypothalamic-pituitary-adrenocortical axis on anxiety-like behavior and metabolic status have been found throughout animal taxa.However,the precise effects of the balancing adrenal corticosteroid biosynthesis under the influence of adrenocorticotrophic hormone(ACTH),a pro-opiomelanocortin(POMC)-derived peptide,on animal energy expenditure and somatic growth remain unknown.POMC has also been identified as one of the candidate loci for polycystic ovary syndrome,which features hyperandrogenism and some prevalence of obesity in patients.Here we show that zebrafish lacking functional POMCa exhibit similar phenotypes of stress response and body weight gain but not obesity as observed in mammalian models.In contrast with the impaired anorexigenic signaling cascade of melanocyte-stimulating hormones and leptin,which are responsible for their obesity-prone weight gain observed in various pome mutant mammals,analyses with our pomca mutant series indicate that ACTH is the key regulator for the phenotype with enhanced somatic growth without obesity in pomca-deficient zebrafish.Hypocortisolism associated with hyperandrogenism has been observed in the pomca-deficient zebrafish,with enhanced activation of mammalian target of rapamycin complex 1;reutilization of amino acids and fatty acid^-oxidation are observed in the muscle tissue of the pomca-deficient fish.After reducing hyperandrogenism by crossing our pomca mutant fish with a cy p l 7a 1-deficient background,the phenotype of enhanced somatic growth in pomca-deficient fish was no longer observed.Thus,our work also demonstrated that the role of POMCa in stress response seems to be conserved in vertebrates,whereas its effect on adipostasis is unique to teleosts.
基金supported by the National Key Research and Development Program of China(2018YFD0900404)the Pilot Program A Project from the Chinese Academy of Sciences(XDA24010206)+2 种基金State Key Laboratory of Freshwater Ecology and Biotechnology(2019FBZ05)the National Natural Science Foundation of China(31530077)China Postdoctoral Science Foundation(2020M682527)。
文摘Improvement in fish feed conversion efficiency(FCE)is beneficial for sustaining global food fish supplies.Here,we show that a set of polymorphisms at locus of the corticotropin releasing hormone receptor 2(crhr2),which is involved in hypothalamuspituitary-interrenal(HPI)axis signaling,is associated with improved FCE in farmed allogynogenetic gibel carp strain CAS Ⅲ compared with that in the wild gibel carp strain Dongting(DT).This set of polymorphisms downregulates the expression levels of crhr2 mRNA in the brain and pituitary tissues in gibel carp strain CAS Ⅲ compared with those in strain DT.Furthermore,compromised HPI axis signaling is observed in gibel carp strain CAS Ⅲ,such as decreased α-melanocyte stimulating hormone protein levels,plasma cortisol content,and stress responses.Moreover,enhanced activation of protein kinase B/mammalian target of rapamycin complex 1 signaling observed in the muscle tissue of strain CAS Ⅲ in comparison to that in strain DT indicated elevated anabolic metabolism in strain CAS Ⅲ.Thus,these studies demonstrate that the genetic markers associated with compromised HPI axis signaling,such as crhr2,are potentially useful for genetic selection toward improvement in farmed fish growth and FCE,which would reduce fishmeal consumption and thereby indirectly facilitate sustainable fisheries.
基金funded by the National Natural Science Foundation of China (Nos. 41804026, 41804024 and 41931075)。
文摘The International GNSS Service(IGS) has been providing reliable Global Ionospheric Maps(GIMs) since 1998. The Ionosphere Associate Analysis Centers(IAACs) model the global ionospheric Total Electron Content(TEC) and generate the daily GIM products within the context of the IGS. However, the rapid and final daily GIM products have a latency of at least one day and one week or so, respectively. This limits the value of GIM products in real-time GNSS applications.We propose and develop an approach for near real-time modeling of global ionospheric TEC by using the hourly IGS data. We perform an experiment in a real operating environment to generate near real-time GIM(named BUHG) products for more than two years. Final daily GIM products,Precise Point Positioning(PPP) based VTEC resources, and JASON-3 Vertical TEC(VTEC) measurements are collected for testing the performance of BUHG. The results show that the performance of BUHG is very close to that of the daily GIM products. Also, there is good agreement between BUHG and PPP-derived VTEC as well as with JASON-3 VTEC. It is possible that BUHG would be further improved with an increase in available hourly GNSS data.