Objective：To identify and isolate CD133 positive cancer stem-like cells （CD133^＋ cells） from the highly invasive human hepatocellular carcinoma cell Iine（MHCC97H）, and examine their potential for clonogenicity a...Objective：To identify and isolate CD133 positive cancer stem-like cells （CD133^＋ cells） from the highly invasive human hepatocellular carcinoma cell Iine（MHCC97H）, and examine their potential for clonogenicity and tumorigenicity. Methods： CD133^＋ and CD133^- cells were isolated from MHCC97H cell line by magnetic bead cell sorting（MACS）, and the potentials of CD133^＋ cells for colony formation and tumorigenicity were evaluated by soft agar cloning and tumor formation following nude mice inoculation. Results：CD133^＋ cells represent a minority（0.5-2.0%） of the tumor cell population with a greater colony-forming efficiency and greater tumor production ability. The colony-forming efficiency of CD133^＋ cells in soft agar was significantly higher than CD133^- cells（36.8 ± 1.4 vs 12.9 ± 0.8, P 〈 0.05）. After 6 weeks, 3/5 mice inoculated with 1 × 10^3 CD133^＋ cells, 4/5 with 1 × 10^4 CD133^＋ cells and 5/5 with 1× 10^5 CD133^＋ cells developed detectable tumors at the injection site, while only one tumor was found in mice treated with same numbers of CD133 cells. Conclusion： CD133 may be a hallmark of liver cancer stem cells （CSC） in human hepatocellular carcinoma（HCC）, because the CD133^＋ cells identified and isolated with anti-CD133 labeled magnetic beads from MHCC97H cell line exhibit high potentials for clonogenicity and tumorigenicity. These CD 133^＋ cells might contribute to hepatocarcinogenesis, as well as the growth and recurrence of human HCC, and therefore may be a useful target for anti-cancer therapy.展开更多
文摘Objective：To identify and isolate CD133 positive cancer stem-like cells （CD133^＋ cells） from the highly invasive human hepatocellular carcinoma cell Iine（MHCC97H）, and examine their potential for clonogenicity and tumorigenicity. Methods： CD133^＋ and CD133^- cells were isolated from MHCC97H cell line by magnetic bead cell sorting（MACS）, and the potentials of CD133^＋ cells for colony formation and tumorigenicity were evaluated by soft agar cloning and tumor formation following nude mice inoculation. Results：CD133^＋ cells represent a minority（0.5-2.0%） of the tumor cell population with a greater colony-forming efficiency and greater tumor production ability. The colony-forming efficiency of CD133^＋ cells in soft agar was significantly higher than CD133^- cells（36.8 ± 1.4 vs 12.9 ± 0.8, P 〈 0.05）. After 6 weeks, 3/5 mice inoculated with 1 × 10^3 CD133^＋ cells, 4/5 with 1 × 10^4 CD133^＋ cells and 5/5 with 1× 10^5 CD133^＋ cells developed detectable tumors at the injection site, while only one tumor was found in mice treated with same numbers of CD133 cells. Conclusion： CD133 may be a hallmark of liver cancer stem cells （CSC） in human hepatocellular carcinoma（HCC）, because the CD133^＋ cells identified and isolated with anti-CD133 labeled magnetic beads from MHCC97H cell line exhibit high potentials for clonogenicity and tumorigenicity. These CD 133^＋ cells might contribute to hepatocarcinogenesis, as well as the growth and recurrence of human HCC, and therefore may be a useful target for anti-cancer therapy.
