Objective: To observe the effect of electroacupuncture(EA) combined with oriented conductive bioprotein hydrogel(OCBH) on the recovery of nerve function in rats with complete spinal cord injury(SCI)and to explore its ...Objective: To observe the effect of electroacupuncture(EA) combined with oriented conductive bioprotein hydrogel(OCBH) on the recovery of nerve function in rats with complete spinal cord injury(SCI)and to explore its effect and mechanism on the formation and changes of glial scars.Methods: A total of 72 female Sprague-Dawley rats were randomly divided into groups according to the treatment received. A rat model of complete SCI was constructed using a spinal cord transection.Behavioral assessments, hematoxylin-eosin(H&E) staining, immunofluorescence staining, and Western blotting were performed at a fixed period after the operation.Results: The material group and the material + EA group obtained better results in the behavioral assessments(all P <.05) and the H&E staining. In the immunofluorescence staining and Western blotting,the GFAP protein was expressed more and denser in the material group and the material + EA group than in the model group, and the density of the GFAP expression in the material + EA group was lower at week 12 than in the material group(all P <.05). The expression of complement C3 in the model, material,and material + EA groups decreased in turn. Some inflammatory factors and the NF-κB signaling pathway showed similar results in the Western blotting(all P <.05). The expression of the GDNF protein in the material + EA group was significantly higher than that in the model group and the material group(both P <.01).Conclusion: EA combined with OCBH can promote the recovery of motor functions after SCI by facilitating the formation of glial scars in the early stage, preventing the further spread of an inflammatory response that would affect the activation of A1/A2 astrocytes and change the morphology of glial scars at the spinal cord-material interface in its late stage.展开更多
To understand the transfer process of soluble reactive phosphorus （SRP） on the lake sediment-water interface in a mesotrophic shallow lake in South China, the SRP concentrations and the oxidation-reduction potential...To understand the transfer process of soluble reactive phosphorus （SRP） on the lake sediment-water interface in a mesotrophic shallow lake in South China, the SRP concentrations and the oxidation-reduction potential （ORP） across the sediment-water interfaces were continually monitored. Sediment samples were collected from Xinghu Lake in Guangdong Province. The ORP dynamics at different layers of overlying water was similar for six experimental systems, whereas those in porewater were significantly different. The ORP in overlying water was 200-300 mV higher than those in sediments. The oxygen penetration depth ranged from 2 to 10 mm in Xiannu Lake sediments. The variation amplitudes of ORP increased with sediment depth, but the mean ORP values were all about 218 mV. The SRP concentrations in porewater maintained at a low level of about 0.049 mg/L because of high atom ratio of total iron and total manganese to total phosphorus. The SRP concentrations and variation amplitudes in porewater increased with sediment depth. The SRP in overlying water mainly originated from S RP transference of the porewater of middle and bottom sediments （3-15 cm）. The ORP variation and SRP transfer in porewater played important roles in changing SRP concentrations. A distinct SRP concentration gradient appeared in overlying water when intense exchange occurred at the sediment-water interface; therefore, it was necessary to monitor the SRP concentration profiles to accurately estimate the internal loading.展开更多
基金financially supported by the National Natural Science Foundation of China (81973882 and 81804119)
文摘Objective: To observe the effect of electroacupuncture(EA) combined with oriented conductive bioprotein hydrogel(OCBH) on the recovery of nerve function in rats with complete spinal cord injury(SCI)and to explore its effect and mechanism on the formation and changes of glial scars.Methods: A total of 72 female Sprague-Dawley rats were randomly divided into groups according to the treatment received. A rat model of complete SCI was constructed using a spinal cord transection.Behavioral assessments, hematoxylin-eosin(H&E) staining, immunofluorescence staining, and Western blotting were performed at a fixed period after the operation.Results: The material group and the material + EA group obtained better results in the behavioral assessments(all P <.05) and the H&E staining. In the immunofluorescence staining and Western blotting,the GFAP protein was expressed more and denser in the material group and the material + EA group than in the model group, and the density of the GFAP expression in the material + EA group was lower at week 12 than in the material group(all P <.05). The expression of complement C3 in the model, material,and material + EA groups decreased in turn. Some inflammatory factors and the NF-κB signaling pathway showed similar results in the Western blotting(all P <.05). The expression of the GDNF protein in the material + EA group was significantly higher than that in the model group and the material group(both P <.01).Conclusion: EA combined with OCBH can promote the recovery of motor functions after SCI by facilitating the formation of glial scars in the early stage, preventing the further spread of an inflammatory response that would affect the activation of A1/A2 astrocytes and change the morphology of glial scars at the spinal cord-material interface in its late stage.
基金supported by the Environment and Pollution Control Technology Innovation Program,which is one part of the 985 Project at Sun Yat-sen University
文摘To understand the transfer process of soluble reactive phosphorus （SRP） on the lake sediment-water interface in a mesotrophic shallow lake in South China, the SRP concentrations and the oxidation-reduction potential （ORP） across the sediment-water interfaces were continually monitored. Sediment samples were collected from Xinghu Lake in Guangdong Province. The ORP dynamics at different layers of overlying water was similar for six experimental systems, whereas those in porewater were significantly different. The ORP in overlying water was 200-300 mV higher than those in sediments. The oxygen penetration depth ranged from 2 to 10 mm in Xiannu Lake sediments. The variation amplitudes of ORP increased with sediment depth, but the mean ORP values were all about 218 mV. The SRP concentrations in porewater maintained at a low level of about 0.049 mg/L because of high atom ratio of total iron and total manganese to total phosphorus. The SRP concentrations and variation amplitudes in porewater increased with sediment depth. The SRP in overlying water mainly originated from S RP transference of the porewater of middle and bottom sediments （3-15 cm）. The ORP variation and SRP transfer in porewater played important roles in changing SRP concentrations. A distinct SRP concentration gradient appeared in overlying water when intense exchange occurred at the sediment-water interface; therefore, it was necessary to monitor the SRP concentration profiles to accurately estimate the internal loading.
