COMTIS:Customizable touchless interaction system for large screen visualization

Background Large screen visualization sys tems have been widely utilized in many industries.Such systems can help illustrate the working states of different production systems.However,efficient interaction with such systems is still a focus of related research.Methods In this paper,we propose a touchless interaction system based on RGB-D camera using a novel bone-length constraining method.The proposed method optimizes the joint data collected from RGB-D cameras with more accurate and more stable results on very noisy data.The user can customize the system by modifying the finite-state machine in the system and reuse the gestures in multiple scenarios,reducing the number of gestures that need to be designed and memorized.Results/Conclusions The authors tested the system in two cases.In the first case,we illustrated a process in which we improved the gesture designs on our system and tested the system through user study.In the second case,we utilized the system in the mining industry and conducted a user study,where users say that they think the system is easy to use.

Sub-diffraction dark spot localization microscopy

Single molecular localization microscopy(SMLM)is a useful tool in biological observation with sub-10-nm resolution.However,SMLM is incapable of discerning two molecules within the diffraction-limited region unless with the help of a stochastic on–off switching scheme which yet entails time-consuming processes.Here,we produce a novel kind of focal spot pattern,called sub-diffraction dark spot(SDS),to localize molecules within the sub-diffraction region of interest.In our proposed technique nominated as sub-diffracted dark spot localization microscopy(SDLM),multiple molecules within the diffraction-limited region could be distinguished without the requirement of stochastic fluorescent switches.We have numerically investigated some related impacts of SDLM,such as detection circle diameter,collected photon number,background noise,and SDS size.Simulative localization framework has been implemented on randomly distributed and specifically structured samples.In either two-or three-dimensional case,SDLM is evidenced to have2 nm localization accuracy.

Dual-modulation difference stimulated emission depletion microscopy to suppress the background signal

Stimulated emission depletion(STED)nanoscopy is one of the most well-developed nanoscopy techniques that can provide subdiffraction spatial resolution imaging.Here,we introduce dual-modulation difference STED microscopy(dmdSTED)to suppress the background noise in traditional STED imaging.By applying respective time-domain modulations to the two continuous-wave lasers,signals are distributed discretely in the frequency spectrum and thus are obtained through lock-in demodulation of the corresponding frequencies.The background signals can be selectively eliminated from the effective signal without compromise of temporal resolution.We used nanoparticle,fixed cell,and perovskite coating experiments,as well as theoretical demonstration,to confirm the effectiveness of this method.We highlight dmdSTED as an idea and approach with simple implementation for improving the imaging quality,which substantially enlarges the versatility of STED nanoscopy.