Contributing to organ formation and tissue regeneration,extracellular matrix(ECM)constituents provide tissue with three-dimensional(3D)structural integrity and cellular-function regulation.Containing the crucial trait...Contributing to organ formation and tissue regeneration,extracellular matrix(ECM)constituents provide tissue with three-dimensional(3D)structural integrity and cellular-function regulation.Containing the crucial traits of the cellular microenvironment,ECM substitutes mediate cell–matrix interactions to prompt stem-cell proliferation and differentiation for 3D organoid construction in vitro or tissue regeneration in vivo.However,these ECMs are often applied generically and have yet to be extensively developed for specific cell types in 3D cultures.Cultured cells also produce rich ECM,particularly stromal cells.Cellular ECM improves 3D culture development in vitro and tissue remodeling during wound healing after implantation into the host as well.Gaining better insight into ECM derived from either tissue or cells that regulate 3D tissue reconstruction or organ regeneration helps us to select,produce,and implant the most suitable ECM and thus promote 3D organoid culture and tissue remodeling for in vivo regeneration.Overall,the decellularization methodologies and tissue/cell-derived ECM as scaffolds or cellular-growth supplements used in cell propagation and differentiation for 3D tissue culture in vitro are discussed.Moreover,current preclinical applications by which ECM components modulate the wound-healing process are reviewed.展开更多
Bis(4-fluorophenyl) substituted oxazole(2,5-Oxz) and C2(5)-C2'(5') linked bioxazole isomers(C2-C2'_BOxz,C2-C5'_BOxz and C5-C5'_BOxz) were concisely synthesized via palladium-catalyzed regioselectiv...Bis(4-fluorophenyl) substituted oxazole(2,5-Oxz) and C2(5)-C2'(5') linked bioxazole isomers(C2-C2'_BOxz,C2-C5'_BOxz and C5-C5'_BOxz) were concisely synthesized via palladium-catalyzed regioselective and sequential C-H arylation in 1-3 reaction steps along with 20%-83% of total yields from oxazole and4-bromofluorobenzene.The linking orientation plays a key role in the packing geometry and photophysical properties of C2-C2'_BOxz,C2-C5'_BOxz and C5-C5'_BOxz.These bioxazole isomers in solid state showed significant differences in photoluminescence quantum yields(PLQY)(0.33,0.25 and 0.04,respectively),delayed fluorescence properties and powder X-ray diffraction(PXRD) patterns,suggesting the divergence in intermolecular interactions.The theoretically calculated gradient isosurfaces and complexation energies indicate the existence of intense π-π interactions between molecular layers,which are in good agreement with the variation trend of optical properties.展开更多
Rheology analysis of granular flows is important for predicting geophysical hazards and designing industrial processes. Using a discrete element method, we simulate simple shear flows in 3D under a constant confining ...Rheology analysis of granular flows is important for predicting geophysical hazards and designing industrial processes. Using a discrete element method, we simulate simple shear flows in 3D under a constant confining pressure of 10 kPa. The inertial number proposed by the GDR MiDi group in France is adopted to distinguish rheology regimes, Both translational and angular velocity profiles are investigated, and both fluid-like and solid-like behavior modes are observed in the flows. The maximum angular velocity occurs near the localized deformation area. We also investigate the energy characteristics of the flows and find that at very small shearing speed, the mean kinetic energy density ek is close to zero, while the mean elastic energy density ec is much greater. At large shearing speed, ek increases. The fluctuating parts of the two types of energy increase with increasing shear speed. Thus, the mean energy density ratio ek/ec can be used in addition to the inertial number to distinguish flow regimes. These results provide insights from energetics into the rheological properties of granular flows.展开更多
基金supported by Research Grants from the National Institutes of Health(No.1R01AR067747)Ming Pei and by NIH/NIAID(Nos.R21AI152832,R03AI165170)to Yuanyuan Zhang.
文摘Contributing to organ formation and tissue regeneration,extracellular matrix(ECM)constituents provide tissue with three-dimensional(3D)structural integrity and cellular-function regulation.Containing the crucial traits of the cellular microenvironment,ECM substitutes mediate cell–matrix interactions to prompt stem-cell proliferation and differentiation for 3D organoid construction in vitro or tissue regeneration in vivo.However,these ECMs are often applied generically and have yet to be extensively developed for specific cell types in 3D cultures.Cultured cells also produce rich ECM,particularly stromal cells.Cellular ECM improves 3D culture development in vitro and tissue remodeling during wound healing after implantation into the host as well.Gaining better insight into ECM derived from either tissue or cells that regulate 3D tissue reconstruction or organ regeneration helps us to select,produce,and implant the most suitable ECM and thus promote 3D organoid culture and tissue remodeling for in vivo regeneration.Overall,the decellularization methodologies and tissue/cell-derived ECM as scaffolds or cellular-growth supplements used in cell propagation and differentiation for 3D tissue culture in vitro are discussed.Moreover,current preclinical applications by which ECM components modulate the wound-healing process are reviewed.
基金the financial support from the National Natural Science Foundation of China(Nos.21801028,11704050,61505015)Scientific Research Project of the Education Department of Sichuan Province(No.2018Z099)Project of Science and Technology Department of Sichuan Province(No.2017FZ0085)。
文摘Bis(4-fluorophenyl) substituted oxazole(2,5-Oxz) and C2(5)-C2'(5') linked bioxazole isomers(C2-C2'_BOxz,C2-C5'_BOxz and C5-C5'_BOxz) were concisely synthesized via palladium-catalyzed regioselective and sequential C-H arylation in 1-3 reaction steps along with 20%-83% of total yields from oxazole and4-bromofluorobenzene.The linking orientation plays a key role in the packing geometry and photophysical properties of C2-C2'_BOxz,C2-C5'_BOxz and C5-C5'_BOxz.These bioxazole isomers in solid state showed significant differences in photoluminescence quantum yields(PLQY)(0.33,0.25 and 0.04,respectively),delayed fluorescence properties and powder X-ray diffraction(PXRD) patterns,suggesting the divergence in intermolecular interactions.The theoretically calculated gradient isosurfaces and complexation energies indicate the existence of intense π-π interactions between molecular layers,which are in good agreement with the variation trend of optical properties.
文摘Rheology analysis of granular flows is important for predicting geophysical hazards and designing industrial processes. Using a discrete element method, we simulate simple shear flows in 3D under a constant confining pressure of 10 kPa. The inertial number proposed by the GDR MiDi group in France is adopted to distinguish rheology regimes, Both translational and angular velocity profiles are investigated, and both fluid-like and solid-like behavior modes are observed in the flows. The maximum angular velocity occurs near the localized deformation area. We also investigate the energy characteristics of the flows and find that at very small shearing speed, the mean kinetic energy density ek is close to zero, while the mean elastic energy density ec is much greater. At large shearing speed, ek increases. The fluctuating parts of the two types of energy increase with increasing shear speed. Thus, the mean energy density ratio ek/ec can be used in addition to the inertial number to distinguish flow regimes. These results provide insights from energetics into the rheological properties of granular flows.
