3D bioprinting of complex biological structures with tunable elastic modulus and porosity using freeform reversible embedding of suspended hydrogels

Three-dimensional(3D)bioprinting has been used widely for the construction of hard tissues such as bone and cartilage.However,constructing soft tissues with complex structures remains a challenge.In this study,complex structures characterized by both tunable elastic modulus and porosity were printed using freeform reversible embedding of suspended hydrogels(FRESHs)printing methods.A mixture of alginate and gelatin was used as the main functional component of the bioink.Rheological analysis showed that this bioink possesses shear thinning and shear recovery properties,supporting both cryogenic and FRESH printing methods.Potential printing capabilities and limitations of cryogenic and FRESH printing were then analyzed by printability tests.A series of complex structures were printed by FRESH printing methods which could not be realized using conventional approaches.Mechanical tests and scanning electron microscopy analysis showed that the printed structure is of excellent flexibility and could be applied in various conditions by adjusting its mechanical modulus and porosity.L929 fibroblast cells maintained cell viability in cell-laden-printed structures,and the addition of collagen further improved the hydrogels’biocompatibility.Overall,all results provided useful insight into the building of human soft tissue organ blocks.

Design Property Network-Based Change Propagation Prediction Approach for Mechanical Product Development

Design changes are unavoidable during mechanical product development; whereas the avalanche propagation of design change imposes severely negative impacts on the design cycle. To improve the validity of the change propagation prediction, a mathematical program- ming model is presented to predict the change propagation impact quantitatively. As the foundation of change propa- gation prediction, a design change analysis model（DCAM） is built in the form of design property network. In DCAM, the connections of the design properties are identified as the design specification, which conform to the small-world network theory. To quantify the change propagation impact, change propagation intensity（CPI） is defined as a quantitative and much more objective assessment metric. According to the characteristics of DCAM, CPI is defined and indicated by tour assessment factors： propagation likelihood, node degree, long-chain linkage, and design margin. Furthermore, the optimal change propagation path is searched with the evolutionary ant colony optimiza- tion（ACO） algorithm, which corresponds to the minimized maximum of accumulated CPI. In practice, the change impact of a gear box is successfully analyzed. The pro- posed change propagation prediction method is verified tobe efficient and effective, which could provide different results according to various the initial changes.

Design and Simulation of Flow Field for Bone Tissue Engineering Sca old Based on Triply Periodic Minimal Surface

A novel method was proposed to design the structure of a bone tissue engineering scafold based on triply periodic minimal surface.In this method,reverse engineering software was used to reconstruct the surface from point cloud data.This method overcomes the limitations of commercially available software packages that prevent them from generating models with complex surfaces used for bone tissue engineering scafolds.Additionally,the fluid feld of the scafolds was simulated through a numerical method based on fnite volume and the cell proliferation performance was evaluated via an in vitro experiment.The cell proliferation and the mass flow evaluated in a bioreactor further verifed the flow feld simulated using computational fluid dynamics.The result of this study illustrates that the pressure value drops rapidly from 0.103 Pa to 0.011 Pa in the y-axis direction and the mass flow is unevenly distributed in the outlets.The mass flow in the side outlets is observed to be approximately 24.3 times higher thanthe bottom.Importantly,although the mean value of wall shear stress is signifcantly more than 0.05 Pa,there is stil a large area with a suitable shear stress below 0.05 Pa where most cells can proliferate well.The result shows that th inlet velocity 0.0075 m/s is suitable for cell proliferation in the scafold.This study provides an insight into the design analysis,and in vitro experiment of a bone tissue engineering scafold.

Effect of Machined Surface Integrity on Fatigue Performance of Metal Workpiece:A Review

Fatigue performance is a serious concern for mechanical components subject to cyclical stresses,particularly where safety is paramount.The fatigue performance of components relies closely on their surface integrity because the fatigue cracks generally initiate from free surfaces.This paper reviewed the published data,which addressed the effects of machined surface integrity on the fatigue performance of metal workpieces.Limitations in existing studies and the future directions in anti-fatigue manufacturing field were proposed.The remarkable surface topography(e.g.,low roughness and few local defects and inclusions)and large compressive residual stress are beneficial to fatigue performance.However,the indicators that describe the effects of surface topography and residual stress accurately need further study and exploration.The effect of residual stress relaxation under cycle loadings needs to be precisely modeled precisely.The effect of work hardening on fatigue performance had two aspects.Work hardening could increase the material yield strength,thereby delaying crack nucleation.However,increased brittleness could accel-erate crack propagation.Thus,finding the effective control mechanism and method of work hardening is urgently needed to enhance the fatigue performance of machined components.The machining-induced metallurgical structure changes,such as white layer,grain refinement,dislocation,and martensitic transformation affect the fatigue performance of a workpiece significantly.However,the unified and exact conclusion needs to be investigated deeply.Finally,different surface integrity factors had complicated reciprocal effects on fatigue performance.As such,studying the comprehensive influence of surface integrity further and establishing the reliable prediction model of workpiece fatigue performance are meaningful for improving reliability of components and reducing test cost.

A new modeling approach for stress-strain relationship taking into account strain hardening and stored energy by compacted graphite iron evolution

Compacted graphite iron(CGI)is considered to be an ideal diesel engine material with excellent physical and mechanical properties,which meet the requirements of energy conservation and emission reduction.However,knowledge of the microstructure evolution of CGI and its impact on flow stress remains limited.In this study,a new modeling approach for the stress–strain relationship is proposed by considering the strain hardening effect and stored energy caused by the microstructure evolution of CGI.The effects of strain,strain rate,and deformation temperature on the microstructure of CGI during compression deformation are examined,including the evolution of graphite morphology and the microstructure of the pearlite matrix.The roundness and fractal dimension of graphite particles under different deformation conditions are measured.Combined with finite element simulation models,the influence of graphite particles on the flow stress of CGI is determined.The distributions of grain boundary and geometrically necessary dislocations(GNDs)density in the pearlite matrix of CGI under different strains,strain rates,and deformation temperatures are analyzed by electron backscatter diffraction technology,and the stored energy under each deformation condition is calculated.Results show that the proportion and amount of low-angle grain boundaries and the average GNDs density increase with the increase of strain and strain rate and decreased first and then increased with an increase in deformation temperature.The increase in strain and strain rate and the decrease in deformation temperature contribute to the accumulation of stored energy,which show similar variation trends to those of GNDs density.The parameters in the stress–strain relationship model are solved according to the stored energy under different deformation conditions.The consistency between the predicted results from the proposed stress–strain relationship and the experimental results shows that the evolution of stored energy can accurately predict the stress–strain relationship of CGI.

Effects of FDM-3D printing parameters on mechanical properties and microstructure of CF/PEEK and GF/PEEK

Fused deposition modeling(FDM)has unique advantages in the rapid prototyping of thermoplastics which have been developed in diverse fields.However,although great efforts have been made to optimize FDM process,the mechanical properties of printed parts are limited by the weak interlamination bonding as well as the poor performance of raw filaments used,such as acrylonitrile butadiene styrene(ABS),polylactic acid(PLA).Adding fibers into thermoplastic matrix and preparing high-performance filaments have been indicated to enhance the properties of fabricated parts.Recently,heat-resistant polyetheretherketone(PEEK)and its fiber reinforced composites were proposed for FDM process due to overcoming the limitation of equipment and process.However,few researches have been reported on the effects of FDM-3 D printing parameters on the mechanical properties of fiber reinforced PEEK composites.Therefore,5 wt%carbon fiber(CF)and glass fiber(GF)reinforced PEEK composite filaments were prepared respectively in this study.The effects of various printing parameters including nozzle temperature,platform temperature,printing speed and layer thickness on the mechanical properties(including tensile strength,flexural strength and impact strength)were surveyed.To analyze the microstructure and failure reasons of printed CF/PEEK and GF/PEEK samples,the tensile fractured surfaces were investigated via scanning electron microscope(SEM).

Nano-enhanced biolubricant in sustainable manufacturing:From processability to mechanisms

To eliminate the negative effect of traditional metal-working fluids and achieve sustainable manufacturing,the usage of nano-enhanced biolubricant(NEBL)is widely researched in minimum quantify lubrication(MQL)machining.It's improved tool wear and surface integrity have been preliminarily verified by experimental studies.The previous review papers also concluded the major influencing factors of processability including nano-enhancer and lubricant types,NEBL concentration,micro droplet size,and so on.Nevertheless,the complex action of NEBL,from preparation,atomization,infiltration to heat transfer and anti-friction,is indistinct which limits preparation of process specifications and popularity in factories.Especially in the complex machining process,in-depth understanding is difficult and meaningful.To fll this gap,this paper concentrates on the comprehensive quantitative assessment of processability based on tribological,thermal,and machined surface quality aspects for NEBL application in turning,milling,and grinding.Then it attempts to answer mechanisms systematically considering multi-factor influence of molecular structure,physicochemical properties,concentration,and dispersion.Firstly,this paper reveals advanced lubrication and heat transfer mechanisms of NEBL by quantitative comparison with biolubricant-based MQL machining.Secondly,the distinctive filmformation,atomization,and infiltration mechanisms of NEBL,as distinguished from metal-working fluid,are clarified combining with its unique molecular structure and physical properties.Furtherly,the process optimization strategy is concluded based on the synergistic relationship analysis among process variables,physicochemical properties,machining mechanisms,and performance of NEBL.Finally,the future development directions are put forward aiming at current performance limitations of NEBL,which requires improvement on preparation and jet methods respects.This paper will help scientists deeply understand effective mechanism,formulate process specifications,and find future development trend of this technology.

Experimental study of surface integrity and fatigue life in the face milling of Inconel 718

The Inconel 718 alloy is widely used in the aerospace and power industries. The machining-induced surface integrity and fatigue life of this material are important factors for consideration due to high reliability and safety requirements. In this work, the milling of Inconel 718 was conducted at different cutting speeds and feed rates. Surface integrity and fatigue life were measured directly. The effects of cutting speed and feed rate on surface integrity and their further influences on fatigue life were analyzed. Within the chosen parameter range, the cutting speed barely affected the surface roughness, whereas the feed rate increased the surface roughness through the ideal residual height. The surface hardness increased as the cutting speed and feed rate increased. Tensile residual stress was observed on the machined surface, which showed improvement with the increasing feed rate. The cutting speed was not an influencing factor on fatigue life, but the feed rate affected fatigue life through the surface roughness. The high surface roughness resulting from the high feed rate could result in a high stress concentration factor and lead to a low fatigue life.

Profile error compensation in precision grinding of ellipsoid optical surface

Optical glass elements with the ellipsoidal surface are used in many critical aerospace instruments,such as satellites,telescope and cameras.Their optical performance is mainly affected by profile accuracy and surface quality.In this paper,a rectangular ellipsoid surface is precisely ground on a BK7 optical glass blank by grating scanning grinding path with a three-axis CNC precision surface grinder.A profile error compensation procedure for ellipsoidal grinding is proposed based on the error analysis about the primary error sources in the XY and YZ projection planes during the grinding process.The mathematical prediction models of the wheel arc profile error,the measurement and wear error of the grinding wheel radiuses are established.By applying the proposed error compensation procedure,the profile accuracy of the ellipsoidal surface was improved from 4 lm to 2 lm in the XY plane,and improved from 15 lm to 5 lm in the YZ plane.

Bonding mechanism and performance of ceramic coatings by sol-gel process

Thin alumina coatings were prepared on carbide tools to enhance their wear-resistant ability by dip coating from an aluminum alkoxide solution. The coating eventually obtained was perfectly integrated, without any macroscopic defect, and showed good performance in turning medium carbon steel, which presented a novel and promising coating method for cutting tools. The coating morphology before and after heat treatment was examined with the aid of a scanning electron microscope (SEM). The composition of coating and interface layer as well as the crystal structure of coating was characterized by X-ray diffraction (XRD). The elemental distribution near the interlayer was analyzed by electron probe microanalysis (EPMA).