Background:Fat deposition is an important economic consideration in pig production.The amount of fat deposition in pigs seriously affects production efficiency,quality,and reproductive performance,while also affecting...Background:Fat deposition is an important economic consideration in pig production.The amount of fat deposition in pigs seriously affects production efficiency,quality,and reproductive performance,while also affecting consumers’choice of pork.Weighted gene co-expression network analysis(WGCNA)is effective in pig genetic studies.Therefore,this study aimed to identify modules that co-express genes associated with fat deposition in pigs(Songliao black and Landrace breeds)with extreme levels of backfat(high and low)and to identify the core genes in each of these modules.Results:We used RNA sequences generated in different pig tissues to construct a gene expression matrix consisting of 12,862 genes from 36 samples.Eleven co-expression modules were identified using WGCNA and the number of genes in these modules ranged from 39 to 3,363.Four co-expression modules were significantly correlated with backfat thickness.A total of 16 genes(RAD9A,IGF2R,SCAP,TCAP,SMYD1,PFKM,DGAT1,GPS2,IGF1,MAPK8,FABP,FABP5,LEPR,UCP3,APOF,and FASN)were associated with fat deposition.Conclusions:RAD9A,TCAP,SMYD1,PFKM,GPS2,and APOF were the key genes in the four modules based on the degree of gene connectivity.Combining these results with those from differential gene analysis,SMYD1 and PFKM were proposed as strong candidate genes for body size traits.This study explored the key genes that regulate porcine fat deposition and lays the foundation for further research into the molecular regulatory mechanisms underlying porcine fat deposition.展开更多
Background： Fatness traits in animals are important for their growth, meat quality, reproductive performance, and immunity. The liver is the principal organ of the regulation of lipid metabolism, and this study used ...Background： Fatness traits in animals are important for their growth, meat quality, reproductive performance, and immunity. The liver is the principal organ of the regulation of lipid metabolism, and this study used massive parallelized high-throughput sequencing technologies to determine the porcine liver tissue transcriptome architecture of two full-sibling Songliao black pigs harboring extremely different phenotypes of backfat thickness. Results： The total number of reads produced for each sample was in the region of 53 million, and 8,226 novel transcripts were detected. Approximately 92 genes were differentially regulated in the liver tissue, while 31 spliced transcripts and 33 primary transcripts showed significantly differential expression between pigs with higher and lower backfat thickness. Genes that were differentially expressed were involved in the metabolism of various substances, smal molecule biochemistry, and molecular transport. Conclusions： Genes involved in the regulation of iipids could play an important role in lipid and fatty acid metabolism in the liver. These results could help us understand how liver metabolism affects the backfat thickness of pigs.展开更多
Pig meat off-flavor is attributed to trimethylamine(TMA)concentration,and it is considered as the precursor of the fishy off-flavor problems.In this study,TMA concentrations in pig meat were determined,and the interac...Pig meat off-flavor is attributed to trimethylamine(TMA)concentration,and it is considered as the precursor of the fishy off-flavor problems.In this study,TMA concentrations in pig meat were determined,and the interactions with breed and gender effects were discussed.In addition,the TMA threshold for meat off-flavor and pig meat natural quality was measured in relation to meat storage and movement,and the influential factors including the pig breed and storage time were discussed.The results indicated positive effects on the precursor of the fishy off-flavor and the TMA threshold.Native breeds were found to have lower TMA concentrations than European breeds(P<0.01),and females and castrated males had significantly lower TMA concentration than males(P<0.01),The threshold concentration of TMA when meat was classed as off-flavored was 25μg·g^(–1),and this occurred after 35–38 h of storage.The natural qualities,such as appearance,flavor,color and overall acceptable scores declined significantly after 4 days in storage(P<0.01).It is concluded that pig meat off-flavor,breed and gender were essential factors affecting flavor for meat breeding programs,and storage time is important for pig meat natural quality.展开更多
基金financially supported by the National Key Research and Development Project(2019YFE0106800)the National Key R&D Program of China(2018YFD0501000)+2 种基金Beijing Municipal Education Commission Science and Technology Program General Project(KM201910020010)Beijing Innovation Consortium of Agriculture Research System(BAIC02-2021)the China Agriculture Research System(CARS-35).
文摘Background:Fat deposition is an important economic consideration in pig production.The amount of fat deposition in pigs seriously affects production efficiency,quality,and reproductive performance,while also affecting consumers’choice of pork.Weighted gene co-expression network analysis(WGCNA)is effective in pig genetic studies.Therefore,this study aimed to identify modules that co-express genes associated with fat deposition in pigs(Songliao black and Landrace breeds)with extreme levels of backfat(high and low)and to identify the core genes in each of these modules.Results:We used RNA sequences generated in different pig tissues to construct a gene expression matrix consisting of 12,862 genes from 36 samples.Eleven co-expression modules were identified using WGCNA and the number of genes in these modules ranged from 39 to 3,363.Four co-expression modules were significantly correlated with backfat thickness.A total of 16 genes(RAD9A,IGF2R,SCAP,TCAP,SMYD1,PFKM,DGAT1,GPS2,IGF1,MAPK8,FABP,FABP5,LEPR,UCP3,APOF,and FASN)were associated with fat deposition.Conclusions:RAD9A,TCAP,SMYD1,PFKM,GPS2,and APOF were the key genes in the four modules based on the degree of gene connectivity.Combining these results with those from differential gene analysis,SMYD1 and PFKM were proposed as strong candidate genes for body size traits.This study explored the key genes that regulate porcine fat deposition and lays the foundation for further research into the molecular regulatory mechanisms underlying porcine fat deposition.
基金financially supported by the innovation research team for modern agricultural industry and technology in Beijing
文摘Background： Fatness traits in animals are important for their growth, meat quality, reproductive performance, and immunity. The liver is the principal organ of the regulation of lipid metabolism, and this study used massive parallelized high-throughput sequencing technologies to determine the porcine liver tissue transcriptome architecture of two full-sibling Songliao black pigs harboring extremely different phenotypes of backfat thickness. Results： The total number of reads produced for each sample was in the region of 53 million, and 8,226 novel transcripts were detected. Approximately 92 genes were differentially regulated in the liver tissue, while 31 spliced transcripts and 33 primary transcripts showed significantly differential expression between pigs with higher and lower backfat thickness. Genes that were differentially expressed were involved in the metabolism of various substances, smal molecule biochemistry, and molecular transport. Conclusions： Genes involved in the regulation of iipids could play an important role in lipid and fatty acid metabolism in the liver. These results could help us understand how liver metabolism affects the backfat thickness of pigs.
基金This work was supported by grants from the National Key Basic Research Program of China(2014CB138500)the Domain-Specific Projects for Transgenic Biologic Breeding(2014ZX08009-002).
文摘Pig meat off-flavor is attributed to trimethylamine(TMA)concentration,and it is considered as the precursor of the fishy off-flavor problems.In this study,TMA concentrations in pig meat were determined,and the interactions with breed and gender effects were discussed.In addition,the TMA threshold for meat off-flavor and pig meat natural quality was measured in relation to meat storage and movement,and the influential factors including the pig breed and storage time were discussed.The results indicated positive effects on the precursor of the fishy off-flavor and the TMA threshold.Native breeds were found to have lower TMA concentrations than European breeds(P<0.01),and females and castrated males had significantly lower TMA concentration than males(P<0.01),The threshold concentration of TMA when meat was classed as off-flavored was 25μg·g^(–1),and this occurred after 35–38 h of storage.The natural qualities,such as appearance,flavor,color and overall acceptable scores declined significantly after 4 days in storage(P<0.01).It is concluded that pig meat off-flavor,breed and gender were essential factors affecting flavor for meat breeding programs,and storage time is important for pig meat natural quality.
