RNA-seq analysis reveals the critical role of the novel lnc RNA BIANCR in intramuscular adipogenesis through the ERK1/2 signaling pathway

Background Long non-coding RNAs(lncRNAs)regulate numerous biological processes,including adipogenesis.Research on adipogenesis will assist in the treatment of human metabolic diseases and improve meat quality in livestock,such as the content of intramuscular fat(IMF).However,the significance of lncRNAs in intramuscular adipogenesis remains unclear.This research aimed to reveal the lncRNAs transcriptomic profiles in the process of bovine intramuscular adipogenesis and to identify the lncRNAs involved in the adipogenesis of bovine intramuscular adipocytes.Results In this research,a landscape of lncRNAs was identified with RNA-seq in bovine intramuscular adipocytes at four adipogenesis stages(0 d,3 d,6 d,and 9 d after differentiation).A total of 7035 lncRNAs were detected,including 3396 novel lncRNAs.Based on the results of differential analysis,co-expression analysis,and functional prediction,we focused on the bovine intramuscular adipogenesis-associated long non-coding RNA(BIANCR),a novel lncRNA that may have an important regulatory function.The knockdown of BIANCR inhibited proliferation and promoted apoptosis of intramuscular preadipocytes.Moreover,BIANCR knockdown inhibited intramuscular adipogenesis by regulating the ERK1/2 signaling pathway.Conclusion This study obtained the landscape of lncRNAs during adipogenesis in bovine intramuscular adipocytes.BIANCR plays a crucial role in adipogenesis through the ERK1/2 signaling pathway.The results are noteworthy for improving beef meat quality,molecular breeding,and metabolic disease research.

Identification of key genes and metabolites involved in meat quality performance in Qinchuan cattle by WGCNA

Understanding the genetic and metabolic elements that impact meat quality is crucial to improving production and meeting consumer demands in the beef sector.Differences in meat quality among various muscle areas in beef cattle can impact pricing in the market.Despite progress in genomics,the specific genes and metabolites that affect meat quality characteristics in Qinchuan cattle remain inadequately understood.Therefore,this study aims to evaluate the meat quality characteristics of four specific muscle locations(tenderloin,striploin,high rib,and ribeye muscles)in Qinchuan bulls,including 10 traits(total protein content(TPC),intramuscular fat(IMF),non-esterified fatty acid(NEFA),meat color(L*,a*,and b*),shear force(SF),cooking loss(CL),pH0,and pH24).This experiment uses transcriptome,metabolome sequencing,and sophisticated analytical methodologies such as weighted gene co-expression network analysis(WGCNA)and protein–protein interaction networks(PPI)to identify the key genes and metabolites associated with specific traits.The findings highlight three notable genes(NDUFAB1,NDUFA12,and NDUFB7)linked to intramuscular fat(IMF),three key genes(CSRP3,ACAA3,and ACADVL)correlated with non-esterified fatty acids(NEFA),and one crucial gene(CREBBP)influencing meat color.In conclusion,this investigation offers a new perspective on the differences in bovine muscle locations and contributes to the molecular understanding of bovine meat quality.Future research endeavors could delve deeper into the identified genes and pathways to enhance beef cattle’s quality and yield.