Dengue virus(DENV) is a mosquito-transmitted virus that is expanding across the world,The incidence of dengue infection,especially severe disease,has been increasing,DENV consist of 4 serotypes of single stranded RNA ...Dengue virus(DENV) is a mosquito-transmitted virus that is expanding across the world,The incidence of dengue infection,especially severe disease,has been increasing,DENV consist of 4 serotypes of single stranded RNA viruses(D1-D4) in the genus Flavivirus,family Flaviviridae,Majority of dengue infections are asymptomatic cases,which cause difficulty in disease control and are important in dengue surveillance,There is still no gold standard to diagnose asymptomatic dengue infection,Plaque reduction neutralization test(PRNT) has been developed for many purposes such as immunological study,clinical study,vaccine trial and is currently the most sensitive and specific method for serological surveillance,However,PRNT shows some degree of cross reaction among different dengue serotypes especially secondary dengue infection cases and to other flaviviruses,Moreover,various modification since the beginning make PRNT lack of inter-laboratory standardization which is an important issue,This paper discusses the important of asymptomatic dengue infection and its diagnostic method.展开更多
Dengue virus (DENV) is the world most prevalent mosquito-transmitted virus. The incidence of dengue infection has been increasing and most of the infected people are asymptomatic or have non specific febrile illness. ...Dengue virus (DENV) is the world most prevalent mosquito-transmitted virus. The incidence of dengue infection has been increasing and most of the infected people are asymptomatic or have non specific febrile illness. Laboratory test is essential to confirm dengue infection in epidemiological studies. We developed an indirect ELISA test based on monoclonal immunoglobulin against all four DENV serotypes and evaluated the test for the diagnosis of asymptomatic dengue infection in paired annual serum samples. The indirect ELISA was found to have a sensitivity 87.5% and specificity 78.3% at optimized cut off. The results from indirect ELISA demonstrate an incidence of asymptomatic dengue infection from children aged 4 - 11 years in Ratchaburi province, Thailand (2006-2009). These findings indicate that the indirect ELISA is suitable for serodiagnosis and seroepidemiological studies of asymptomatic dengue infection.展开更多
基金supported by Faculty of Tropical Medicine,Mahidol University,Thailand
文摘Dengue virus(DENV) is a mosquito-transmitted virus that is expanding across the world,The incidence of dengue infection,especially severe disease,has been increasing,DENV consist of 4 serotypes of single stranded RNA viruses(D1-D4) in the genus Flavivirus,family Flaviviridae,Majority of dengue infections are asymptomatic cases,which cause difficulty in disease control and are important in dengue surveillance,There is still no gold standard to diagnose asymptomatic dengue infection,Plaque reduction neutralization test(PRNT) has been developed for many purposes such as immunological study,clinical study,vaccine trial and is currently the most sensitive and specific method for serological surveillance,However,PRNT shows some degree of cross reaction among different dengue serotypes especially secondary dengue infection cases and to other flaviviruses,Moreover,various modification since the beginning make PRNT lack of inter-laboratory standardization which is an important issue,This paper discusses the important of asymptomatic dengue infection and its diagnostic method.
文摘Dengue virus (DENV) is the world most prevalent mosquito-transmitted virus. The incidence of dengue infection has been increasing and most of the infected people are asymptomatic or have non specific febrile illness. Laboratory test is essential to confirm dengue infection in epidemiological studies. We developed an indirect ELISA test based on monoclonal immunoglobulin against all four DENV serotypes and evaluated the test for the diagnosis of asymptomatic dengue infection in paired annual serum samples. The indirect ELISA was found to have a sensitivity 87.5% and specificity 78.3% at optimized cut off. The results from indirect ELISA demonstrate an incidence of asymptomatic dengue infection from children aged 4 - 11 years in Ratchaburi province, Thailand (2006-2009). These findings indicate that the indirect ELISA is suitable for serodiagnosis and seroepidemiological studies of asymptomatic dengue infection.
