This study aimed to identify the potential metabolic biomarkers of the Badagan constitution(BC) in Mongolian medicine. Serum samples from the participants with BCs(n = 32) and aggregative constitutions(n = 30) were an...This study aimed to identify the potential metabolic biomarkers of the Badagan constitution(BC) in Mongolian medicine. Serum samples from the participants with BCs(n = 32) and aggregative constitutions(n = 30) were analyzed by using ultrahigh-performance liquid chromatography/mass spectrometry. Meanwhile, principal component analysis and orthogonal partial least squares discriminant analysis were used to characterize the endogenous metabolites and potential biomarkers, respectively. Fifteen of the 18 biomarkers in six metabolic pathways were significantly upregulated,including phosphatidylserine, sphingolipids, tryptophan, riboflavin and glutathione, and three biomarkers were significantly downregulated,including lysophosphatidylcholine(LysoPC)(18:1), LysoPC(16:1), and lysophosphatidylethanolamine(LysoPE)(22:2). This study also implied that sphingolipid metabolism, glycerophospholipid metabolism, and tryptophan metabolism played important roles in the BC. Therefore, metabolomics may improve the diagnostic efficacy of the BC in terms of the accuracy and comprehensiveness of a diagnosis based on this constitution. This result further reveals the mechanism of the constitution type in Mongolian medicine and provides a reference for the treatment of related diseases.展开更多
基金supported by the National Natural Science Foundation of China(grant number 81660828)the Open Fund Project of Engineering Technology Research Center of Mongolian Medicine of Inner Mongolia(grant numbers MDK2018070,MDK2019034)+2 种基金MDK2019036National and local Joint Engineering Research Center for Mongolian Medicine Research Open Fund Projects(MDK2021035)the Natural Science Foundation of Inner Mongolia of China(grant number 2019MS08040)。
文摘This study aimed to identify the potential metabolic biomarkers of the Badagan constitution(BC) in Mongolian medicine. Serum samples from the participants with BCs(n = 32) and aggregative constitutions(n = 30) were analyzed by using ultrahigh-performance liquid chromatography/mass spectrometry. Meanwhile, principal component analysis and orthogonal partial least squares discriminant analysis were used to characterize the endogenous metabolites and potential biomarkers, respectively. Fifteen of the 18 biomarkers in six metabolic pathways were significantly upregulated,including phosphatidylserine, sphingolipids, tryptophan, riboflavin and glutathione, and three biomarkers were significantly downregulated,including lysophosphatidylcholine(LysoPC)(18:1), LysoPC(16:1), and lysophosphatidylethanolamine(LysoPE)(22:2). This study also implied that sphingolipid metabolism, glycerophospholipid metabolism, and tryptophan metabolism played important roles in the BC. Therefore, metabolomics may improve the diagnostic efficacy of the BC in terms of the accuracy and comprehensiveness of a diagnosis based on this constitution. This result further reveals the mechanism of the constitution type in Mongolian medicine and provides a reference for the treatment of related diseases.
