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Numerical modeling of flow in continuous bends from Daliushu to Shapotou in Yellow River 被引量:2
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作者 He-fang JING chun-guang li +2 位作者 Ya-kun GUO li-jun ZHU Yi-tian li 《Water Science and Engineering》 EI CAS CSCD 2014年第2期194-207,共14页
The upper reach of the Yellow River from Daliushu to Shapotou consists of five bends and has complex topography. A two-dimensional Re-Normalisation Group (RNG) k-ε model was developed to simulate the flow in the re... The upper reach of the Yellow River from Daliushu to Shapotou consists of five bends and has complex topography. A two-dimensional Re-Normalisation Group (RNG) k-ε model was developed to simulate the flow in the reach. In order to take the circulation currents in the bends into account, the momentum equations were improved by adding an additional source term. Comparison of the numerical simulation with field measurements indicates that the improved two-dimensional depth-averaged RNG k-e model can improve the accuracy of the numerical simulation. A rapid adaptive algorithm was constructed, which can automatically adjust Manning's roughness coefficient in different parts of the study river reach. As a result, not only can the trial computation time be significantly shortened, but the accuracy of the numerical simulation can also be greatly improved. Comparison of the simulated and measured water surface slopes for four typical cases shows that the longitudinal and transverse slopes of the water surface increase with the average velocity upstream. In addition, comparison was made between the positions of the talweg and the main streamline, which coincide for most of the study river reach. However, deviations between the positions of the talweg and the main streamline were found at the junction of two bends, at the position where the river width suddenly decreases or increases. 展开更多
关键词 numerical simulation RNG k-e model Yellow River continuous bend circulationflow adaptive algorithm regarding Manning's roughness coefficient
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运动激活自噬对小鼠骨骼肌抗氧化防御功能的影响 被引量:1
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作者 王平 chun-guang li +4 位作者 崔迪 邱守涛 漆正堂 李娅晖 丁树哲 《中国运动医学杂志》 CAS CSCD 北大核心 2018年第1期49-56,共8页
目的:探讨一次性力竭运动能否激活骨骼肌自噬及运动性自噬对骨骼肌抗氧化防御功能的影响,为运动性自噬与骨骼肌抗氧化防御功能之间的发生机制提供实验依据。方法:30只健康雄性4周龄C57BL/6小鼠随机分为安静对照组(n=6)和运动组(n=24),... 目的:探讨一次性力竭运动能否激活骨骼肌自噬及运动性自噬对骨骼肌抗氧化防御功能的影响,为运动性自噬与骨骼肌抗氧化防御功能之间的发生机制提供实验依据。方法:30只健康雄性4周龄C57BL/6小鼠随机分为安静对照组(n=6)和运动组(n=24),运动组进行一次性力竭跑台运动,分别于运动完成后的0 h(n=6)、6 h(n=6)、12 h(n=6)和24 h(n=6)取小鼠两侧腓肠肌,采用黄嘌呤氧化酶法测定腓肠肌锰超氧化物歧化酶(Mn SOD)、总超氧化物歧化酶(T-SOD)和铜锌超氧化物歧化酶(Cuzn SOD)的活性;比色法测定腓肠肌总抗氧化能力(T-AOC)的含量;荧光定量PCR和Western Blot分别检测腓肠肌自噬相关因子Beclin1、P62和B淋巴细胞瘤-2(Bcl2)的m RNA和蛋白表达。结果:与安静对照组相比,(1)小鼠腓肠肌Beclin1 m RNA表达在运动后0 h、6 h均极显著升高(P<0.01),P62 m RNA表达在运动后24 h极显著升高(P<0.01);P62蛋白表达在运动后0 h、12 h和24 h均显著下降(P<0.05或P<0.01),Bcl2蛋白表达在运动后0 h、6 h和12 h均显著升高(P<0.05或P<0.01);(2)Mn SOD和Cuzn SOD活性在0 h均显著升高(P<0.05),T-SOD活性在6 h极显著下降(P<0.01),T-AOC含量在运动后6 h、12 h、24 h均显著升高(P<0.05或P<0.01);P62蛋白表达与T-AOC含量呈负相关。结论:一次性力竭跑台运动可在一定程度上激活骨骼肌自噬相关因子Beclin1 m RNA和P62蛋白的表达,可适度提高自噬;且自噬相关因子P62蛋白表达与骨骼肌抗氧化防御功能具有相关性,这可能是运动增加骨骼肌抗氧化防御功能的分子机理之一。 展开更多
关键词 运动 自噬 骨骼肌 抗氧化防御功能
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急性递增负荷跑台运动对小鼠腓肠肌自噬和葡萄糖转运功能的影响
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作者 王平 chun-guang li +1 位作者 崔迪 丁树哲 《中国运动医学杂志》 CAS CSCD 北大核心 2021年第1期19-28,共10页
目的:观察急性递增负荷跑台运动对腓肠肌自噬和葡萄糖转运功能的影响,探讨运动激活自噬对骨骼肌葡萄糖转运功能的作用。方法:健康雄性4周龄ICR小鼠适应性喂养3天,然后随机分为安静后根据恢复时间再分为运动后即刻组(0 h组,n=6只)、运动... 目的:观察急性递增负荷跑台运动对腓肠肌自噬和葡萄糖转运功能的影响,探讨运动激活自噬对骨骼肌葡萄糖转运功能的作用。方法:健康雄性4周龄ICR小鼠适应性喂养3天,然后随机分为安静后根据恢复时间再分为运动后即刻组(0 h组,n=6只)、运动后6 h组(6 h组,n=6只)、运动后12 h组(12m/min、持续30 min/day适应性训练3天,然后按照坡度为0°、速度为8.2 m/min、相当于53%VO_(2max)、持续15 min的1级负荷,坡度为5°、速度为15 m/min、相当于64%VO_(2max)、持续15 min的2级负荷和坡度为10°、速度为19.3 m/min、相当于76%VO_(2max)的3级负荷依次递增,增加到3级负荷时,保持此负荷直至力竭。运动力竭后在对应时间点取小鼠腓肠肌和心脏血(安静组小鼠在安静状态下取材)。采用实时荧光定量PCR(qRT-PCR)测定腓肠肌自噬相关基因13(Atg13)、微管相关蛋白1轻链3(LC3)、自噬相关蛋白p62Atg13、Ⅱ型微管相关蛋白1轻链3(LC3Ⅱ)、Ⅱ和Ⅰ型微管相关蛋白1轻链3比值(LC3Ⅱ/LC3Ⅰ)、p62、LAMP2和GLUT4的蛋白表达;采用葡萄糖试剂盒检测小鼠血糖,肌糖原试剂盒(比色法)检测腓肠肌肌糖mRNA、LC3 mRNA、LC3Ⅱ蛋白表达和LC3Ⅱ/LC3Ⅰ蛋白表达均显著增加(P<0.01或P<0.05),0 h组、12h组和24 h组小鼠腓肠肌p62蛋白表达均显著下降(P<0.01或P<0.05);2)急性递增负荷跑台运动6 h组、12 h组和24 h组血糖均极显著下降(P<0.01),0h组和6h组腓肠肌肌糖原含量均极显著下降(P<0.01);台运动可激活腓肠肌自噬相关基因Atg13 mRNA、LAMP2 mRNA、LC3 mRNA、LC3Ⅱ蛋白和LC3Ⅱ/LC3GLUT4的表达;自噬相关因子LC3Ⅱ和LAMP2的蛋白表达与葡萄糖转运功能均具有相关性,这种关联可能是急性运动调节骨骼肌葡萄糖转运功能的重要分子机制之一。 展开更多
关键词 运动 骨骼肌 自噬 葡萄糖转运功能
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Regulating metal-acid double site balance on mesoporous SiO_(2)-Al_(2)O_(3) composite oxide for supercritical n-decane cracking
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作者 liu-ling Chen Jun Zhang +5 位作者 chun-guang li li-Qun Fei Bo Wang Chen-Qi Zhang Yi Jiao Jian-li Wang 《Petroleum Science》 SCIE EI CAS CSCD 2024年第4期2881-2891,共11页
The balance between metal and acid sites directly affects the preparation of high-performance cracking catalysts with high heat sink and low coking.Nevertheless,how to control acid-metal sites balance and its relation... The balance between metal and acid sites directly affects the preparation of high-performance cracking catalysts with high heat sink and low coking.Nevertheless,how to control acid-metal sites balance and its relationship with cracking performance are reported scarcely.In this work,a series of Pt/Al_(2)O_(3)-SiO_(2) dual sites catalysts with different metal to acid active sites ratio(C_(M)/C_(SA))were constructed by ethanolassisted impregnation method and the impact on n-decane cracking under supercritical conditions was systematically and deeply investigated.The results showed that the conversion and carbon deposition increased gradually with varied C_(M)/C_(SA)and reached the balance at C_(M)/C_(SA)of 0.13.The proper ratio C_(M)/C_(SA)(0.13)can balance the deep dehydrogenation coking over metal active sites and high heat sink of cracking over acid active sites,the chemical heat sink reaches amazing 1.75 MJ/kg and carbon deposition is only22.03 mg/cm^(2) at 750℃.Meanwhile,the few metal sites at low C_(M)/C_(SA)and the few strong acid sites at high C_(M)/C_(SA)are the main factors limiting the cracking activity.Low C_(M)/C_(SA)limit the activation of C-H bond and deep dehydrogenation of coking precursor,resulting in relative low cracking activity and carbon deposition,while high C_(M)/C_(SA)limit the activation of C-C bond and increase the deep dehydrogenation.In this contribution,design and construction of metal-acid dual sites can not only provide the technical solution for the preparation of high heat sink and low coking cracking catalyst,but also deepen the understanding of the cracking path of hydrocarbon fuel. 展开更多
关键词 Metal-acid dual sites balance Catalytic cracking Hydrocarbon fuels Chemical heat sink Carbon deposition
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线粒体非折叠蛋白反应对运动性骨骼肌代谢稳态的调节作用
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作者 樊晓 chun-guang li 王平 《中国运动医学杂志》 CAS CSCD 北大核心 2018年第4期353-357,共5页
线粒体非折叠蛋白反应(UPRmt)是一种适应性应激反应通路,在运动性骨骼肌代谢稳态中起重要作用。UPRmt在运动、组织缺氧、氧化应激、禁食、钙离子稳态失衡和肌肉收缩刺激等能量应激下,将线粒体基质积累或产生的大量未折叠或错误折叠的蛋... 线粒体非折叠蛋白反应(UPRmt)是一种适应性应激反应通路,在运动性骨骼肌代谢稳态中起重要作用。UPRmt在运动、组织缺氧、氧化应激、禁食、钙离子稳态失衡和肌肉收缩刺激等能量应激下,将线粒体基质积累或产生的大量未折叠或错误折叠的蛋白质,通过上调核基因编码的线粒体分子伴侣热激蛋白60(HSP60)、热激蛋白70(HSP70)的表达,帮助发生错误折叠的蛋白恢复正常蛋白构象及协助新合成的蛋白发生正确折叠,将信号从线粒体转导至细胞核,此过程有助于维持线粒体内蛋白质的动态平衡和细胞存活,从而保证线粒体蛋白组的最佳质量和功能,维持线粒体功能完整,保证骨骼肌代谢稳态。运动训练可通过UPRmt使线粒体网络结构功能重塑,也可使线粒体蛋白的质量控制稳定,进一步巩固和优化线粒体功能,维持骨骼肌质量和功能完整性,从而稳定骨骼肌代谢功能的稳态,同时还能有效防治神经退行性疾病、肌肉功能异常、肥胖和II型糖尿病等代谢疾病的发生,也为线粒体等相关疾病的病理机制及防治措施提供新的理论依据。 展开更多
关键词 线粒体非折叠蛋白反应 UPRmt 运动 骨骼肌 代谢稳态
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Investigation of open channel flow with unsubmerged rigid vegetation by the lattice Boltzmann method 被引量:7
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作者 He-fang Jing Yin-juan Cai +3 位作者 Wei-hong Wang Yakun Guo chun-guang li Yu-chuan Bai 《Journal of Hydrodynamics》 SCIE EI CSCD 2020年第4期771-783,共13页
The aquatic vegetation can significantly affect the flow structure,the sediment transport,the bed scour and the water quality in rivers,lakes,reservoirs and open channels.In this study,the lattice Boltzmann method(LBM... The aquatic vegetation can significantly affect the flow structure,the sediment transport,the bed scour and the water quality in rivers,lakes,reservoirs and open channels.In this study,the lattice Boltzmann method(LBM)is applied in the two-dimensional numerical simulation of the flow structure in a flume with rigid vegetation.A multi-relaxation time model is applied to improve the stability of the numerical scheme for flows with a high Reynolds number.The vegetation induced drag force is added in the lattice Boltzmann equation model in order to improve the simulation accuracy and an algorithm of the multi-relaxation time is developed.Numerical simulations are performed for a wide range of flow and vegetation conditions and are validated by comparing with the laboratory experiments.Analysis of the simulated and experimentally measured flow Helds shows that the numerical simulation can satisfactorily reproduce the laboratory experiments,indicating that the proposed lattice Boltzmann model enjoys a high accuracy for simulating the flow-vegetation interaction in open channels. 展开更多
关键词 Lattice Boltzmann method(LBM) multi-relaxation time model aquatic vegetation drag force open channel flow
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Flow characteristics in open channels with aquatic rigid vegetation 被引量:4
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作者 Yan-jie Wu He-fang Jing +1 位作者 chun-guang li Ying-ting Song 《Journal of Hydrodynamics》 SCIE EI CSCD 2020年第6期1100-1108,共9页
In order to study the flow characteristics in water bodies with rigid aquatic vegetation,series of laboratory experiments are carried out in an open channel,in which glass rods are used as plants with diameters of 6mm... In order to study the flow characteristics in water bodies with rigid aquatic vegetation,series of laboratory experiments are carried out in an open channel,in which glass rods are used as plants with diameters of 6mm,8mm and 10mm,respectively.For each diameter of glass rods,four typical cases are considered with various densities and arrangements of glass rods.The flow velocities in the four cases are measured by the 3-D laser Doppler velocimeter(LDV).The water surface slope,the flow velocity,the water head loss,the vegetation drag force and the hydraulic slope are calculated,analyzed and discussed.The horizontal,vertical and total vegetation densities in the vegetation area are defined and the relationship between these physical parameters and the water surface slope are studied.The head loss and the hydraulic slope in the vegetation area are also calculated,compared and analyzed.It is indicated that the water surface slope and velocity,the head loss and the hydraulic slope in the vegetation area have a close relationship with the arrangement,the density,and the plant diameter of the vegetation. 展开更多
关键词 Aquatic rigid vegetation flow velocity laser Doppler velocimeter(LDV) head loss hydraulic slope
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